HPLC/MS Method for Analyzing Lipid Oxidation Products

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary product of lipid oxidation that is believed to contribute to the formation of a chromogen in the thiobarbituric acid test?

  • Ketone
  • Malonaldehyde (correct)
  • Hydroperoxide
  • Aldehyde

At what wavelength is the chromogen produced in the thiobarbituric acid test typically measured?

  • 350 nm
  • 450 nm (correct)
  • 650 nm
  • 750 nm

What type of spectroscopy is used to detect short-lived free radicals in rancid oils and fats?

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Infrared Spectroscopy
  • Ultraviolet-Visible (UV-Vis) Spectroscopy
  • Electron Spin Resonance (ESR) Spectroscopy (correct)

What is the minimum concentration of free radicals required for detection using ESR Spectroscopy?

<p>10-8 M (C)</p> Signup and view all the answers

What is the wavelength at which hydroperoxides absorb in IR Spectroscopy?

<p>2.93 µm (B)</p> Signup and view all the answers

What is the advantage of IR Spectroscopy in the study of lipid oxidation?

<p>Simple, fast, and requires minimal sample (D)</p> Signup and view all the answers

What is the product of lipid oxidation that is indicated by the absorption band at 5.72 µm in IR Spectroscopy?

<p>Aldehyde (C)</p> Signup and view all the answers

Why does the absorption band at 2.93 µm in IR Spectroscopy eventually disappear?

<p>Due to the replacement of double bonds with hydrogen (D)</p> Signup and view all the answers

What is the role of thiobarbituric acid in the detection of lipid oxidation?

<p>It reacts with aldehydes to form a chromogen (A)</p> Signup and view all the answers

Which spectroscopy technique is used to detect unusual functional groups in fatty acids?

<p>Infrared (IR) Spectroscopy (A)</p> Signup and view all the answers

Flashcards are hidden until you start studying

More Like This

Use Quizgecko on...
Browser
Browser