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HPLC/MS Method for Analyzing Lipid Oxidation Products

10 Questions

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HPLC/MS Method for Analyzing Lipid Oxidation Products

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Questions and Answers

What is the primary product of lipid oxidation that is believed to contribute to the formation of a chromogen in the thiobarbituric acid test?

Ketone

Malonaldehyde (correct)

Hydroperoxide

Aldehyde

At what wavelength is the chromogen produced in the thiobarbituric acid test typically measured?

350 nm

450 nm (correct)

650 nm

750 nm

What type of spectroscopy is used to detect short-lived free radicals in rancid oils and fats?

Nuclear Magnetic Resonance (NMR) Spectroscopy

Infrared Spectroscopy

Ultraviolet-Visible (UV-Vis) Spectroscopy

Electron Spin Resonance (ESR) Spectroscopy (correct)

What is the minimum concentration of free radicals required for detection using ESR Spectroscopy?

10-8 M Signup and view all the answers

What is the wavelength at which hydroperoxides absorb in IR Spectroscopy?

2.93 µm Signup and view all the answers

What is the advantage of IR Spectroscopy in the study of lipid oxidation?

Simple, fast, and requires minimal sample Signup and view all the answers

What is the product of lipid oxidation that is indicated by the absorption band at 5.72 µm in IR Spectroscopy?

Aldehyde Signup and view all the answers

Why does the absorption band at 2.93 µm in IR Spectroscopy eventually disappear?

Due to the replacement of double bonds with hydrogen Signup and view all the answers

What is the role of thiobarbituric acid in the detection of lipid oxidation?

It reacts with aldehydes to form a chromogen Signup and view all the answers

Which spectroscopy technique is used to detect unusual functional groups in fatty acids?

Infrared (IR) Spectroscopy Signup and view all the answers