10 Questions
What is the primary product of lipid oxidation that is believed to contribute to the formation of a chromogen in the thiobarbituric acid test?
Malonaldehyde
At what wavelength is the chromogen produced in the thiobarbituric acid test typically measured?
450 nm
What type of spectroscopy is used to detect short-lived free radicals in rancid oils and fats?
Electron Spin Resonance (ESR) Spectroscopy
What is the minimum concentration of free radicals required for detection using ESR Spectroscopy?
10-8 M
What is the wavelength at which hydroperoxides absorb in IR Spectroscopy?
2.93 µm
What is the advantage of IR Spectroscopy in the study of lipid oxidation?
Simple, fast, and requires minimal sample
What is the product of lipid oxidation that is indicated by the absorption band at 5.72 µm in IR Spectroscopy?
Aldehyde
Why does the absorption band at 2.93 µm in IR Spectroscopy eventually disappear?
Due to the replacement of double bonds with hydrogen
What is the role of thiobarbituric acid in the detection of lipid oxidation?
It reacts with aldehydes to form a chromogen
Which spectroscopy technique is used to detect unusual functional groups in fatty acids?
Infrared (IR) Spectroscopy
This quiz covers the development of a normal phase HPLC/MS method for detecting non-volatile triacylglyceride oxidation products, including epoxy-triacylglyceride, oxo-triacylglyceride, and others. It's suitable for those interested in lipid analysis and chromatography techniques. The method is useful for characterizing and quantifying non-volatile lipid oxidation products.
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