How much do you know about Capillary Electrophoresis for DNA Sequencing?

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Questions and Answers

What is the basis of separation in capillary electrophoresis?

  • Size (correct)
  • Shape
  • Charge
  • Color

What is the diameter of the capillary used in capillary electrophoresis?

  • Nanometer (correct)
  • Millimeter
  • Micrometer
  • Centimeter

What is the purpose of the resin in the capillary in capillary electrophoresis?

  • To separate the molecules (correct)
  • To amplify the template
  • To color-code the bases
  • To detect the type of base

What happens when a labeled fragment passes through the autodetector in capillary electrophoresis?

<p>It records the type of base based on color (C)</p> Signup and view all the answers

Is the product in PCR reaction used as a template in the next cycle?

<p>No (C)</p> Signup and view all the answers

What happens to the speed of movement of a fragment in capillary electrophoresis as its size decreases?

<p>It speeds up (B)</p> Signup and view all the answers

What is the basis of detection of the type of base in capillary electrophoresis?

<p>Color (B)</p> Signup and view all the answers

Is the amplification of template in PCR reaction geometric?

<p>No (D)</p> Signup and view all the answers

What is the amount of sample needed in capillary electrophoresis?

<p>Small amount (C)</p> Signup and view all the answers

What is the relationship between the size of a fragment and its speed of movement in capillary electrophoresis?

<p>Inversely proportional (D)</p> Signup and view all the answers

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