V10_Capillary Electrophoresis on a Chip Quiz
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Questions and Answers

What determines the size of the injection zone in capillary electrophoresis on a chip?

  • Sample outlet dimensions
  • Buffer size in the detection zone
  • Electrokinetic effects
  • Geometry of the channel intersection (correct)

Which method results in superior resolution in capillary electrophoresis on a chip?

  • Plug injection (correct)
  • Stack injection
  • Electrokinetic injection
  • Pressure driven injection

Why are conventional pumps not preferred for sample injection in capillary electrophoresis on a chip?

  • Low detection signal
  • Inability to control flow rate
  • High fluid resistance in small capillaries (correct)
  • Superior resolution

In capillary electrophoresis on a glass chip, what is the purpose of the channels labeled as outlets?

<p>Buffer release (D)</p> Signup and view all the answers

What is one of the main advantages of using electrokinetic injection for sample introduction in capillary electrophoresis?

<p>Controlled flow rate independently of capillary dimensions (C)</p> Signup and view all the answers

Why is detection signal lower with plug injection compared to stack injection in capillary electrophoresis on a chip?

<p>To enhance resolution (D)</p> Signup and view all the answers

What is the main focus of Capillary Isoelectric Focusing (cIEF)?

<p>Analyzing protein charge heterogeneity (A)</p> Signup and view all the answers

Which analytical technique is specifically mentioned for the analysis of up to 96 samples in the context provided?

<p>LabChip GXII Touch HT (A)</p> Signup and view all the answers

In the lecture focused on Capillary Electrophoresis, which system is NOT mentioned for the analysis?

<p>Bio-Rad (C)</p> Signup and view all the answers

What is the main advantage of Capillary Electrophoresis on a Chip over traditional methods?

<p>Improved separation efficiency (D)</p> Signup and view all the answers

Which type of system is used for Capillary Electrophoresis on a Chip according to the provided information?

<p>Agilent Bio-Rad Perkin Elmer 4200 TapeStation system (D)</p> Signup and view all the answers

What does 'WS 23' refer to in the context of the lecture by Prof.Dr.-Ing.Uwe Schnakenberg?

<p>Winter semester lecture series (B)</p> Signup and view all the answers

In capillary electrophoresis on a chip, what determines the size of the injection zone?

<p>The injection time and the electrophoretic mobility of ions in the sample (C)</p> Signup and view all the answers

Which component is responsible for the continuous stacking of the sample into the separation channel during injection time in capillary electrophoresis on a chip?

<p>Sample inlet (D)</p> Signup and view all the answers

In capillary isoelectric focusing (cIEF), what is the primary factor that determines the separation of analytes?

<p>Their isoelectric points (D)</p> Signup and view all the answers

What is the purpose of the electrodes in capillary electrophoresis on a chip?

<p>To provide the driving force for electrophoretic separation (A)</p> Signup and view all the answers

In capillary electrophoresis, what is the significance of the variance ($\sigma^2$) of the initial sample plug?

<p>It determines the resolution of the separation (B)</p> Signup and view all the answers

What is the purpose of the detector window in capillary electrophoresis?

<p>To detect the separated analytes (C)</p> Signup and view all the answers

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