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V10_Capillary Electrophoresis on a Chip Quiz
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V10_Capillary Electrophoresis on a Chip Quiz

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Questions and Answers

What determines the size of the injection zone in capillary electrophoresis on a chip?

  • Sample outlet dimensions
  • Buffer size in the detection zone
  • Electrokinetic effects
  • Geometry of the channel intersection (correct)
  • Which method results in superior resolution in capillary electrophoresis on a chip?

  • Plug injection (correct)
  • Stack injection
  • Electrokinetic injection
  • Pressure driven injection
  • Why are conventional pumps not preferred for sample injection in capillary electrophoresis on a chip?

  • Low detection signal
  • Inability to control flow rate
  • High fluid resistance in small capillaries (correct)
  • Superior resolution
  • In capillary electrophoresis on a glass chip, what is the purpose of the channels labeled as outlets?

    <p>Buffer release</p> Signup and view all the answers

    What is one of the main advantages of using electrokinetic injection for sample introduction in capillary electrophoresis?

    <p>Controlled flow rate independently of capillary dimensions</p> Signup and view all the answers

    Why is detection signal lower with plug injection compared to stack injection in capillary electrophoresis on a chip?

    <p>To enhance resolution</p> Signup and view all the answers

    What is the main focus of Capillary Isoelectric Focusing (cIEF)?

    <p>Analyzing protein charge heterogeneity</p> Signup and view all the answers

    Which analytical technique is specifically mentioned for the analysis of up to 96 samples in the context provided?

    <p>LabChip GXII Touch HT</p> Signup and view all the answers

    In the lecture focused on Capillary Electrophoresis, which system is NOT mentioned for the analysis?

    <p>Bio-Rad</p> Signup and view all the answers

    What is the main advantage of Capillary Electrophoresis on a Chip over traditional methods?

    <p>Improved separation efficiency</p> Signup and view all the answers

    Which type of system is used for Capillary Electrophoresis on a Chip according to the provided information?

    <p>Agilent Bio-Rad Perkin Elmer 4200 TapeStation system</p> Signup and view all the answers

    What does 'WS 23' refer to in the context of the lecture by Prof.Dr.-Ing.Uwe Schnakenberg?

    <p>Winter semester lecture series</p> Signup and view all the answers

    In capillary electrophoresis on a chip, what determines the size of the injection zone?

    <p>The injection time and the electrophoretic mobility of ions in the sample</p> Signup and view all the answers

    Which component is responsible for the continuous stacking of the sample into the separation channel during injection time in capillary electrophoresis on a chip?

    <p>Sample inlet</p> Signup and view all the answers

    In capillary isoelectric focusing (cIEF), what is the primary factor that determines the separation of analytes?

    <p>Their isoelectric points</p> Signup and view all the answers

    What is the purpose of the electrodes in capillary electrophoresis on a chip?

    <p>To provide the driving force for electrophoretic separation</p> Signup and view all the answers

    In capillary electrophoresis, what is the significance of the variance ($\sigma^2$) of the initial sample plug?

    <p>It determines the resolution of the separation</p> Signup and view all the answers

    What is the purpose of the detector window in capillary electrophoresis?

    <p>To detect the separated analytes</p> Signup and view all the answers

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