Podcast
Questions and Answers
What type of biological research does the HDLM-2 cell line primarily support?
What type of biological research does the HDLM-2 cell line primarily support?
The HDLM-2 cell line was established from a female patient diagnosed with Hodgkin lymphoma.
The HDLM-2 cell line was established from a female patient diagnosed with Hodgkin lymphoma.
False
What subtype of Hodgkin lymphoma does the HDLM-2 cell line represent?
What subtype of Hodgkin lymphoma does the HDLM-2 cell line represent?
Nodular sclerosing subtype
The effects of _____ on Hodgkin lymphoma cell lines were investigated to determine their sensitivity.
The effects of _____ on Hodgkin lymphoma cell lines were investigated to determine their sensitivity.
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Match the HDLM-2 cell line with its description:
Match the HDLM-2 cell line with its description:
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At what ECP concentration was a plateau effect observed for HDLM-2?
At what ECP concentration was a plateau effect observed for HDLM-2?
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Higher concentrations of ECP consistently reduced sensitivity in the HDLM-2 cell line.
Higher concentrations of ECP consistently reduced sensitivity in the HDLM-2 cell line.
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BSL–1 biosafety classification is based on _____ guidelines.
BSL–1 biosafety classification is based on _____ guidelines.
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Study Notes
Hodgkin Lymphoma (HL) Cell Line HDLM-2
- HDLM-2 cell line established from pleural effusion of a man with nodular sclerosing HL (stage IV) in 1982 (74 years old).
- Represents a common HL subtype.
- Valuable for studying HL biology, pathogenesis, and therapeutic responses.
- Used to investigate genetic/molecular alterations and signaling pathways involved in HL development.
- Extensive studies have characterized the line for in vitro and in vivo investigations.
Biosafety Considerations
- BSL-1 classification based on U.S. Public Health Service guidelines
- Customer responsibility for facility compliance with biosafety regulations relevant to the country.
Effect of Eosinophil Cationic Protein (ECP) on HL Cell Lines
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Tumor eosinophilia in HL linked to poor prognosis.
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ECP effects on HL tumor cells in vitro investigated.
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HDLM-2 cell line highly sensitive to ECP at low concentrations compared to L428 and KMH2.
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At higher ECP concentrations, a plateau effect was observed for HDLM-2, while L428 and KMH2 showed more pronounced reduction in sensitivity index (SI).
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Reduction in SI for HDLM-2 detected at 0.004 μM ECP (p < 0.01).
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A 70% plateau in SI reached at 0.018 μM ECP. Statistically insignificant difference in SI at higher ECP concentrations (up to 1.14 μM) compared to 0.018 μM.
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72-hour incubation with ECP (0.07 μM) followed by fresh medium:
- No notable injury to surviving HDLM-2, evidenced by continued exponential cell growth after ECP removal.
Citation and Figure Information
- Creative Bioarray catalog numbers should be cited properly in publications.
- PubMed ID of published papers can be submitted for coupon.
- Figures 1 and 2 (Glimelius I, et al., 2011): Illustrate cytotoxic effects of LMW-ECP II on various HL cell lines (including HDLM-2) under different conditions.
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Description
Explore the significance of the HDLM-2 cell line in the study of Hodgkin Lymphoma (HL) biology and treatment responses. This quiz delves into its establishment, biosafety considerations, and the impact of Eosinophil Cationic Protein on HL tumor cells. Test your knowledge on the genetic and molecular aspects of this common HL subtype.
