Hodgkin Lymphoma HL Cell Line HDLM-2 Study

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Questions and Answers

What type of biological research does the HDLM-2 cell line primarily support?

  • Neurological studies
  • Studies of Hodgkin lymphoma (correct)
  • Cardiovascular research
  • Microbial studies

The HDLM-2 cell line was established from a female patient diagnosed with Hodgkin lymphoma.

False (B)

What subtype of Hodgkin lymphoma does the HDLM-2 cell line represent?

Nodular sclerosing subtype

The effects of _____ on Hodgkin lymphoma cell lines were investigated to determine their sensitivity.

<p>eosinophil cationic protein (ECP)</p> Signup and view all the answers

Match the HDLM-2 cell line with its description:

<p>HDLM-2 = Established from pleural effusion of a patient with Hodgkin lymphoma ECP = A protein that affects tumor cells Hodgkin lymphoma = Characterized by nodular masses in lymph nodes Nodular sclerosing subtype = One of the most common subtypes of HL</p> Signup and view all the answers

At what ECP concentration was a plateau effect observed for HDLM-2?

<p>0.018 μM (A)</p> Signup and view all the answers

Higher concentrations of ECP consistently reduced sensitivity in the HDLM-2 cell line.

<p>False (B)</p> Signup and view all the answers

BSL–1 biosafety classification is based on _____ guidelines.

<p>U.S. Public Health Service</p> Signup and view all the answers

Flashcards

HDLM-2 Cell Line

Hodgkin lymphoma (HL) cell line derived from a patient with nodular sclerosing subtype of the disease.

Nodular Sclerosing HL

A subtype of Hodgkin lymphoma characterized by large tumor masses within lymph nodes and scarring in the surrounding tissues.

Eosinophil Cationic Protein (ECP)

A protein released by eosinophils that can affect the growth and behavior of tumor cells.

Survival Index (SI)

A measure of cell viability or growth after treatment with a specific agent, such as ECP.

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Plateau Effect

The point at which increasing the concentration of a substance, like ECP, no longer has a significant effect on the outcome.

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Tumor Eosinophilia

An increase in the number of eosinophils in the tumor tissue, which is associated with a worse outcome.

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In Vitro Study

A type of laboratory experiment that is conducted using cells grown in culture.

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In Vivo Study

Studies that are conducted in living organisms, such as animals, to observe the effects of a treatment or intervention.

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Study Notes

Hodgkin Lymphoma (HL) Cell Line HDLM-2

  • HDLM-2 cell line established from pleural effusion of a man with nodular sclerosing HL (stage IV) in 1982 (74 years old).
  • Represents a common HL subtype.
  • Valuable for studying HL biology, pathogenesis, and therapeutic responses.
  • Used to investigate genetic/molecular alterations and signaling pathways involved in HL development.
  • Extensive studies have characterized the line for in vitro and in vivo investigations.

Biosafety Considerations

  • BSL-1 classification based on U.S. Public Health Service guidelines
  • Customer responsibility for facility compliance with biosafety regulations relevant to the country.

Effect of Eosinophil Cationic Protein (ECP) on HL Cell Lines

  • Tumor eosinophilia in HL linked to poor prognosis.

  • ECP effects on HL tumor cells in vitro investigated.

  • HDLM-2 cell line highly sensitive to ECP at low concentrations compared to L428 and KMH2.

  • At higher ECP concentrations, a plateau effect was observed for HDLM-2, while L428 and KMH2 showed more pronounced reduction in sensitivity index (SI).

  • Reduction in SI for HDLM-2 detected at 0.004 μM ECP (p < 0.01).

  • A 70% plateau in SI reached at 0.018 μM ECP. Statistically insignificant difference in SI at higher ECP concentrations (up to 1.14 μM) compared to 0.018 μM.

  • 72-hour incubation with ECP (0.07 μM) followed by fresh medium:

    • No notable injury to surviving HDLM-2, evidenced by continued exponential cell growth after ECP removal.

Citation and Figure Information

  • Creative Bioarray catalog numbers should be cited properly in publications.
  • PubMed ID of published papers can be submitted for coupon.
  • Figures 1 and 2 (Glimelius I, et al., 2011): Illustrate cytotoxic effects of LMW-ECP II on various HL cell lines (including HDLM-2) under different conditions.

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