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Questions and Answers
Who first observed a drop of pond water and described various forms of bacteria and microorganisms?
Who first observed a drop of pond water and described various forms of bacteria and microorganisms?
- Ross Harrison
- Anton von Leeuwenhoek (correct)
- Wilhelm Roux
- Peyton Rous
Who is considered the 'Father of cell culture'?
Who is considered the 'Father of cell culture'?
- Montrose Burrows
- Ross Harrison (correct)
- Wilhelm Roux
- Anton von Leeuwenhoek
Who maintained chicken embryo cells for 34 years?
Who maintained chicken embryo cells for 34 years?
- Robert Hooke
- Montrose Burrows
- Alexis Carrell (correct)
- Wilton Earle
Who developed the first practical cell culture flask known as the D-flask?
Who developed the first practical cell culture flask known as the D-flask?
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Who was the Englishman who discovered cells using a crude light microscope?
Who was the Englishman who discovered cells using a crude light microscope?
Who is known as the 'Father of cell culture'?
Who is known as the 'Father of cell culture'?
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Who developed the first practical cell culture flask known as the D-flask?
Who developed the first practical cell culture flask known as the D-flask?
Who observed a drop of pond water and described various forms of bacteria and microorganisms?
Who observed a drop of pond water and described various forms of bacteria and microorganisms?
Who was the Englishman who discovered cells using a crude light microscope?
Who was the Englishman who discovered cells using a crude light microscope?
Who maintained chicken embryo cells for 34 years?
Who maintained chicken embryo cells for 34 years?
Who grew a fragment of nerve cord from a frog tadpole in a drop of lymph fluid?
Who grew a fragment of nerve cord from a frog tadpole in a drop of lymph fluid?
Who used plasma to grow explants from various adult tissue and malignant tissue, developing aseptic techniques?
Who used plasma to grow explants from various adult tissue and malignant tissue, developing aseptic techniques?
Who developed T-flask and perforated cellophane as cell substrate, adapted L-cell to grow as monolayer?
Who developed T-flask and perforated cellophane as cell substrate, adapted L-cell to grow as monolayer?
Who is considered to have discovered cells, observing honeycomb-like network of 'cells'?
Who is considered to have discovered cells, observing honeycomb-like network of 'cells'?
Who developed a protocol to freeze cells and keep them at –196ºC for years?
Who developed a protocol to freeze cells and keep them at –196ºC for years?
Who performed the first culture of human cells from malignant cervical cancer cells of Hendrietta Lacks?
Who performed the first culture of human cells from malignant cervical cancer cells of Hendrietta Lacks?
Who showed how genetic traits are passed down to progeny through breeding experiments between strains of peas differing in defined characteristics?
Who showed how genetic traits are passed down to progeny through breeding experiments between strains of peas differing in defined characteristics?
Who concluded that the genetic material is DNA using bacteriophage labelled with 32P and 35S?
Who concluded that the genetic material is DNA using bacteriophage labelled with 32P and 35S?
Who, in 1953, established that the DNA structure is a double Helix using X-ray diffraction data, genetic code, and Central Dogma?
Who, in 1953, established that the DNA structure is a double Helix using X-ray diffraction data, genetic code, and Central Dogma?
Who developed the first practical cell culture flask known as the D-flask?
Who developed the first practical cell culture flask known as the D-flask?
Who demonstrated that a non-virulent strain of bacteria became virulent when mixed with their heat-killed pathogenic counterpart?
Who demonstrated that a non-virulent strain of bacteria became virulent when mixed with their heat-killed pathogenic counterpart?
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Study Notes
Discovery of Cells and Microorganisms
- Antonie van Leeuwenhoek observed a drop of pond water and described various forms of bacteria and microorganisms.
- Robert Hooke, an Englishman, discovered cells using a crude light microscope.
Development of Cell Culture
- Alexis Carrel is known as the 'Father of cell culture' and maintained chicken embryo cells for 34 years.
- Ross Harrison grew a fragment of nerve cord from a frog tadpole in a drop of lymph fluid.
- Carrel used plasma to grow explants from various adult tissue and malignant tissue, developing aseptic techniques.
- Eagle developed T-flask and perforated cellophane as cell substrate, adapted L-cell to grow as monolayer.
Cell Culture Techniques
- Thomas Strangeways demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture.
- Victor Earle developed the first practical cell culture flask known as the D-flask.
Genetics
- Gregor Mendel showed how genetic traits are passed down to progeny through breeding experiments between strains of peas differing in defined characteristics.
- Alfred Hershey and Martha Chase concluded that the genetic material is DNA using bacteriophage labelled with 32P and 35S.
- James Watson and Francis Crick established that the DNA structure is a double Helix using X-ray diffraction data, genetic code, and Central Dogma.
Bacterial Genetic Transformation
- Frederick Griffith demonstrated that a non-virulent strain of bacteria became virulent when mixed with their heat-killed pathogenic counterpart.
Cell Preservation
- Unknown scientist developed a protocol to freeze cells and keep them at –196ºC for years.
Cancer Cell Culture
- George Gey performed the first culture of human cells from malignant cervical cancer cells of Henrietta Lacks.
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