Podcast
Questions and Answers
Who first observed a drop of pond water and described various forms of bacteria and microorganisms?
Who first observed a drop of pond water and described various forms of bacteria and microorganisms?
Who is considered the 'Father of cell culture'?
Who is considered the 'Father of cell culture'?
Who maintained chicken embryo cells for 34 years?
Who maintained chicken embryo cells for 34 years?
Who developed the first practical cell culture flask known as the D-flask?
Who developed the first practical cell culture flask known as the D-flask?
Signup and view all the answers
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Signup and view all the answers
Who was the Englishman who discovered cells using a crude light microscope?
Who was the Englishman who discovered cells using a crude light microscope?
Signup and view all the answers
Who is known as the 'Father of cell culture'?
Who is known as the 'Father of cell culture'?
Signup and view all the answers
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Signup and view all the answers
Who developed the first practical cell culture flask known as the D-flask?
Who developed the first practical cell culture flask known as the D-flask?
Signup and view all the answers
Who observed a drop of pond water and described various forms of bacteria and microorganisms?
Who observed a drop of pond water and described various forms of bacteria and microorganisms?
Signup and view all the answers
Who was the Englishman who discovered cells using a crude light microscope?
Who was the Englishman who discovered cells using a crude light microscope?
Signup and view all the answers
Who maintained chicken embryo cells for 34 years?
Who maintained chicken embryo cells for 34 years?
Signup and view all the answers
Who grew a fragment of nerve cord from a frog tadpole in a drop of lymph fluid?
Who grew a fragment of nerve cord from a frog tadpole in a drop of lymph fluid?
Signup and view all the answers
Who used plasma to grow explants from various adult tissue and malignant tissue, developing aseptic techniques?
Who used plasma to grow explants from various adult tissue and malignant tissue, developing aseptic techniques?
Signup and view all the answers
Who developed T-flask and perforated cellophane as cell substrate, adapted L-cell to grow as monolayer?
Who developed T-flask and perforated cellophane as cell substrate, adapted L-cell to grow as monolayer?
Signup and view all the answers
Who is considered to have discovered cells, observing honeycomb-like network of 'cells'?
Who is considered to have discovered cells, observing honeycomb-like network of 'cells'?
Signup and view all the answers
Who developed a protocol to freeze cells and keep them at –196ºC for years?
Who developed a protocol to freeze cells and keep them at –196ºC for years?
Signup and view all the answers
Who performed the first culture of human cells from malignant cervical cancer cells of Hendrietta Lacks?
Who performed the first culture of human cells from malignant cervical cancer cells of Hendrietta Lacks?
Signup and view all the answers
Who showed how genetic traits are passed down to progeny through breeding experiments between strains of peas differing in defined characteristics?
Who showed how genetic traits are passed down to progeny through breeding experiments between strains of peas differing in defined characteristics?
Signup and view all the answers
Who concluded that the genetic material is DNA using bacteriophage labelled with 32P and 35S?
Who concluded that the genetic material is DNA using bacteriophage labelled with 32P and 35S?
Signup and view all the answers
Who, in 1953, established that the DNA structure is a double Helix using X-ray diffraction data, genetic code, and Central Dogma?
Who, in 1953, established that the DNA structure is a double Helix using X-ray diffraction data, genetic code, and Central Dogma?
Signup and view all the answers
Who developed the first practical cell culture flask known as the D-flask?
Who developed the first practical cell culture flask known as the D-flask?
Signup and view all the answers
Who demonstrated that a non-virulent strain of bacteria became virulent when mixed with their heat-killed pathogenic counterpart?
Who demonstrated that a non-virulent strain of bacteria became virulent when mixed with their heat-killed pathogenic counterpart?
Signup and view all the answers
Study Notes
Discovery of Cells and Microorganisms
- Antonie van Leeuwenhoek observed a drop of pond water and described various forms of bacteria and microorganisms.
- Robert Hooke, an Englishman, discovered cells using a crude light microscope.
Development of Cell Culture
- Alexis Carrel is known as the 'Father of cell culture' and maintained chicken embryo cells for 34 years.
- Ross Harrison grew a fragment of nerve cord from a frog tadpole in a drop of lymph fluid.
- Carrel used plasma to grow explants from various adult tissue and malignant tissue, developing aseptic techniques.
- Eagle developed T-flask and perforated cellophane as cell substrate, adapted L-cell to grow as monolayer.
Cell Culture Techniques
- Thomas Strangeways demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture.
- Victor Earle developed the first practical cell culture flask known as the D-flask.
Genetics
- Gregor Mendel showed how genetic traits are passed down to progeny through breeding experiments between strains of peas differing in defined characteristics.
- Alfred Hershey and Martha Chase concluded that the genetic material is DNA using bacteriophage labelled with 32P and 35S.
- James Watson and Francis Crick established that the DNA structure is a double Helix using X-ray diffraction data, genetic code, and Central Dogma.
Bacterial Genetic Transformation
- Frederick Griffith demonstrated that a non-virulent strain of bacteria became virulent when mixed with their heat-killed pathogenic counterpart.
Cell Preservation
- Unknown scientist developed a protocol to freeze cells and keep them at –196ºC for years.
Cancer Cell Culture
- George Gey performed the first culture of human cells from malignant cervical cancer cells of Henrietta Lacks.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Test your knowledge of the history of cell culture techniques with this quiz. Learn about the pioneering experiments of Wilhelm Roux, Ross Harrison, Montrose Burrows, and Alexis Carrel.
