23 Questions
Who first observed a drop of pond water and described various forms of bacteria and microorganisms?
Anton von Leeuwenhoek
Who is considered the 'Father of cell culture'?
Ross Harrison
Who maintained chicken embryo cells for 34 years?
Alexis Carrell
Who developed the first practical cell culture flask known as the D-flask?
Alexis carrel
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Peyton Rous
Who was the Englishman who discovered cells using a crude light microscope?
Robert Hooke
Who is known as the 'Father of cell culture'?
Ross Harrison
Who demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture?
Peyton Rous
Who developed the first practical cell culture flask known as the D-flask?
Alexis Carrell
Who observed a drop of pond water and described various forms of bacteria and microorganisms?
Anton von Leeuwenhoek
Who was the Englishman who discovered cells using a crude light microscope?
Robert Hooke
Who maintained chicken embryo cells for 34 years?
Alexis Carrell
Who grew a fragment of nerve cord from a frog tadpole in a drop of lymph fluid?
Ross Harrison
Who used plasma to grow explants from various adult tissue and malignant tissue, developing aseptic techniques?
Montrose Burrows
Who developed T-flask and perforated cellophane as cell substrate, adapted L-cell to grow as monolayer?
Wilton Earle
Who is considered to have discovered cells, observing honeycomb-like network of 'cells'?
Robert Hooke
Who developed a protocol to freeze cells and keep them at –196ºC for years?
Alan Parks
Who performed the first culture of human cells from malignant cervical cancer cells of Hendrietta Lacks?
George Gey
Who showed how genetic traits are passed down to progeny through breeding experiments between strains of peas differing in defined characteristics?
Martha Chase
Who concluded that the genetic material is DNA using bacteriophage labelled with 32P and 35S?
Oswald T. Avery
Who, in 1953, established that the DNA structure is a double Helix using X-ray diffraction data, genetic code, and Central Dogma?
James D. Watson
Who developed the first practical cell culture flask known as the D-flask?
George Gey
Who demonstrated that a non-virulent strain of bacteria became virulent when mixed with their heat-killed pathogenic counterpart?
Fredrick Griffith
Study Notes
Discovery of Cells and Microorganisms
- Antonie van Leeuwenhoek observed a drop of pond water and described various forms of bacteria and microorganisms.
- Robert Hooke, an Englishman, discovered cells using a crude light microscope.
Development of Cell Culture
- Alexis Carrel is known as the 'Father of cell culture' and maintained chicken embryo cells for 34 years.
- Ross Harrison grew a fragment of nerve cord from a frog tadpole in a drop of lymph fluid.
- Carrel used plasma to grow explants from various adult tissue and malignant tissue, developing aseptic techniques.
- Eagle developed T-flask and perforated cellophane as cell substrate, adapted L-cell to grow as monolayer.
Cell Culture Techniques
- Thomas Strangeways demonstrated the first use of the proteolytic enzyme trypsin to suspend cell clots for sub-culture.
- Victor Earle developed the first practical cell culture flask known as the D-flask.
Genetics
- Gregor Mendel showed how genetic traits are passed down to progeny through breeding experiments between strains of peas differing in defined characteristics.
- Alfred Hershey and Martha Chase concluded that the genetic material is DNA using bacteriophage labelled with 32P and 35S.
- James Watson and Francis Crick established that the DNA structure is a double Helix using X-ray diffraction data, genetic code, and Central Dogma.
Bacterial Genetic Transformation
- Frederick Griffith demonstrated that a non-virulent strain of bacteria became virulent when mixed with their heat-killed pathogenic counterpart.
Cell Preservation
- Unknown scientist developed a protocol to freeze cells and keep them at –196ºC for years.
Cancer Cell Culture
- George Gey performed the first culture of human cells from malignant cervical cancer cells of Henrietta Lacks.
Test your knowledge of the history of cell culture techniques with this quiz. Learn about the pioneering experiments of Wilhelm Roux, Ross Harrison, Montrose Burrows, and Alexis Carrel.
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