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Questions and Answers
Which of the following is true about cell culture?
Which type of cells are maintained in culture by adhering to the substrate?
Which type of cells are typically maintained in suspension cultures?
Which of the following is NOT a physical property of the cell culture environment?
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Which of the following is NOT a main medium used in cell culture?
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What are the most significant components of the gas phase in cell culture?
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What is the main difficulty in establishing cell lines?
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What does cell culture refer to?
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What is the purpose of using containers in cell culture?
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What are some examples of adherent cells in cell culture?
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Study Notes
Cell Culture Basics
- Cell culture refers to the process of growing cells outside of their natural environment, typically in a controlled laboratory setting.
Types of Cells in Culture
- Adherent cells, such as fibroblasts and epithelial cells, are maintained in culture by adhering to the substrate.
- Suspension cells, such as lymphocytes and blood cells, are typically maintained in suspension cultures.
Cell Culture Environment
- The gas phase in cell culture consists mainly of oxygen, carbon dioxide, and nitrogen.
- Temperature, pH, and osmolarity are physical properties of the cell culture environment.
- Humidity is not a physical property of the cell culture environment.
Cell Culture Media
- The main media used in cell culture are Dulbecco's Modified Eagle Medium (DMEM), Roswell Park Memorial Institute (RPMI), and Minimum Essential Medium (MEM).
- Yeast extract is not a main medium used in cell culture.
Establishing Cell Lines
- The main difficulty in establishing cell lines is the initial isolation and adaptation of cells to in vitro conditions.
Containers in Cell Culture
- Containers are used in cell culture to provide a surface for cell growth and to maintain a sterile environment.
Examples of Adherent Cells
- Fibroblasts and epithelial cells are examples of adherent cells in cell culture.
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Description
Test your knowledge on cell cultures with this quiz! Learn about the growth and proliferation of cellular models under controlled conditions, the components of a culture medium, and the equipment used to maintain optimal conditions.
