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Questions and Answers
Which of the following is true about cell culture?
Which of the following is true about cell culture?
- Cell culture is performed on natural media prepared by mixing equipment.
- Cell culture is the growth and proliferation of cellular models under controlled conditions. (correct)
- Cell culture does not require controlled physico-chemical conditions.
- Cell culture is performed in open containers.
Which type of cells are maintained in culture by adhering to the substrate?
Which type of cells are maintained in culture by adhering to the substrate?
- Immune cells
- Muscle cells
- Nerve cells (correct)
- Blood cells
Which type of cells are typically maintained in suspension cultures?
Which type of cells are typically maintained in suspension cultures?
- Immune cells
- Nerve cells
- Blood cells (correct)
- Muscle cells
Which of the following is NOT a physical property of the cell culture environment?
Which of the following is NOT a physical property of the cell culture environment?
Which of the following is NOT a main medium used in cell culture?
Which of the following is NOT a main medium used in cell culture?
What are the most significant components of the gas phase in cell culture?
What are the most significant components of the gas phase in cell culture?
What is the main difficulty in establishing cell lines?
What is the main difficulty in establishing cell lines?
What does cell culture refer to?
What does cell culture refer to?
What is the purpose of using containers in cell culture?
What is the purpose of using containers in cell culture?
What are some examples of adherent cells in cell culture?
What are some examples of adherent cells in cell culture?
Flashcards
What is cell culture?
What is cell culture?
Growing and multiplying cells in a controlled environment.
What are adherent cells?
What are adherent cells?
Cells that attach to a surface
What are suspension cells?
What are suspension cells?
Cells that float freely in the culture medium.
What is the gas phase in cell culture?
What is the gas phase in cell culture?
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What is cell culture medium?
What is cell culture medium?
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What are the physical properties of cell culture?
What are the physical properties of cell culture?
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What is the main difficulty in establishing cell lines?
What is the main difficulty in establishing cell lines?
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Why are containers used in cell culture?
Why are containers used in cell culture?
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Why are cell cultures important?
Why are cell cultures important?
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What is isolation in cell culture?
What is isolation in cell culture?
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Study Notes
Cell Culture Basics
- Cell culture refers to the process of growing cells outside of their natural environment, typically in a controlled laboratory setting.
Types of Cells in Culture
- Adherent cells, such as fibroblasts and epithelial cells, are maintained in culture by adhering to the substrate.
- Suspension cells, such as lymphocytes and blood cells, are typically maintained in suspension cultures.
Cell Culture Environment
- The gas phase in cell culture consists mainly of oxygen, carbon dioxide, and nitrogen.
- Temperature, pH, and osmolarity are physical properties of the cell culture environment.
- Humidity is not a physical property of the cell culture environment.
Cell Culture Media
- The main media used in cell culture are Dulbecco's Modified Eagle Medium (DMEM), Roswell Park Memorial Institute (RPMI), and Minimum Essential Medium (MEM).
- Yeast extract is not a main medium used in cell culture.
Establishing Cell Lines
- The main difficulty in establishing cell lines is the initial isolation and adaptation of cells to in vitro conditions.
Containers in Cell Culture
- Containers are used in cell culture to provide a surface for cell growth and to maintain a sterile environment.
Examples of Adherent Cells
- Fibroblasts and epithelial cells are examples of adherent cells in cell culture.
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Description
Test your knowledge on cell cultures with this quiz! Learn about the growth and proliferation of cellular models under controlled conditions, the components of a culture medium, and the equipment used to maintain optimal conditions.
