Histopathology Tissue Level of Structural Organisation Quiz

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Microphotography was already well known before the mid-20th century.

True

Cells are naturally colorful and opaque, so they do not need to be stained for observation under a microscope.

False

Camillo Golgi and Santiago Ramon y Cajal shared the Nobel Peace Prize in Physiology or Medicine in 1906 for their work in neuroscience and histology.

True

The Antoni van Leeuwenhoek microscope was used to observe objects that are very large.

False Signup and view all the answers

The development of synthetic dyes for textile industry between 1600 and 1800 did not impact the staining techniques used in histology.

False Signup and view all the answers

The Antoni van Leeuwenhoek microscope was a crude but functional optical instrument used to observe objects that are very small.

True Signup and view all the answers

Microphotography revolutionized microscopy science before the mid-20th century.

False Signup and view all the answers

The development of synthetic dyes between 1600 and 1800 did not impact tissue staining techniques in histology.

False Signup and view all the answers

Most cells are naturally colorful and opaque in their original state, making staining unnecessary for observation under a microscope.

False Signup and view all the answers

Histology was first recognized as a medical specialty in 1906 by Camillo Golgi and Santiago Ramon y Cajal's pioneering work in neuroscience.

True Signup and view all the answers

Understanding the normal structure of tissues is crucial for interpreting changes that occur during exams.

False Signup and view all the answers

Histological techniques allow for the understanding of cell and tissue structure.

True Signup and view all the answers

Pathology focuses on recognizing the normal appearance of several pathological tissues.

False Signup and view all the answers

The development of the cellular doctrine by Theodor Schwann was a key 19th-century histological discovery.

True Signup and view all the answers

Synthetic dyes developed between 1600 and 1800 had no impact on tissue staining techniques in histology.

False Signup and view all the answers

What is the main focus of histopathology?

Interpreting changes that occur during disease Signup and view all the answers

Which histological technique is essential for understanding cell and tissue structure?

Preparation of microscope sections Signup and view all the answers

What did Theodor Schwann propose in 1839 that is significant in histological discoveries?

Cells and tissues are composed of countless single cells Signup and view all the answers

Which type of tissue makes up the organs of the human body?

A combination of epithelia, connective tissue, nervous tissue, and muscle Signup and view all the answers

In histopathology, what is the primary focus when evaluating cells and tissues?

Recognition of both normal and pathologic structure and function Signup and view all the answers

Enzyme histochemistry is the best approach to identify and localize a specific protein within cells.

False Signup and view all the answers

In histologic technique, in situ hybridization is used to visualize nucleic acids.

True Signup and view all the answers

Immunohistochemistry is a technique that uses antibodies to detect specific proteins in cells or tissues.

True Signup and view all the answers

FISH can be used to detect the t(9;22) translocation in leukemia patients.

True Signup and view all the answers

Biopsy samples are often analyzed using histochemical techniques to study tissue structure.

False Signup and view all the answers

Immunohistochemistry can be used to detect and localize specific proteins in cells or tissues.

True Signup and view all the answers

Prussian blue stain is commonly used to detect iron in tissues during histological examinations.

True Signup and view all the answers

Biopsy samples are not often analyzed using ancillary tests like molecular studies or flow cytometry.

False Signup and view all the answers

The source of tissue for cytologic assessment during histological examinations is primarily bone marrow smear aspiration.

False Signup and view all the answers

In situ hybridization involves directly applying nucleic acid sequences to prepared cells and tissue sections.

True Signup and view all the answers

What is the study of cells called?

Cytology Signup and view all the answers

Which component of a cell serves as the structural framework?

Cytoskeleton Signup and view all the answers

What is the fluid component of the cell called?

Cytosol Signup and view all the answers

Which type of tissue is responsible for covering body surfaces and lining body cavities?

Epithelial tissue Signup and view all the answers

Which tissue mainly consists of cells that perform the main function of an organ?

Parenchyma Signup and view all the answers

Which immunocytochemistry method uses a labeled secondary antibody?

Indirect immunocytochemistry Signup and view all the answers

What type of label is directly attached to the antibody in direct immunocytochemistry?

Fluorescent compound Signup and view all the answers

How is the protein of interest on the tissue section visualized in direct immunocytochemistry?

By binding specifically to antigen against which they were produced Signup and view all the answers

What is the key feature of indirect immunocytochemistry that contributes to the amplification of protein labeling?

The labeled secondary antibody binding to primary antibodies Signup and view all the answers

What role does the secondary antibody play in indirect immunocytochemistry?

It binds to the labeled primary antibody Signup and view all the answers

Direct immunocytochemistry uses a labeled primary antibody to bind specifically to the tissue protein of interest.

False Signup and view all the answers

Indirect immunocytochemistry amplifies the labeling of the protein of interest due to the ability of one labeled secondary antibody to bind multiple primary antibodies.

True Signup and view all the answers

Indirect immunocytochemistry uses a secondary antibody made in the same species as the primary antibody against the tissue protein.

False Signup and view all the answers

Direct immunocytochemistry involves applying a secondary antibody labeled with a fluorescent compound or peroxidase to bind to the tissue protein of interest.

False Signup and view all the answers

In indirect immunocytochemistry, the primary antibody binds directly to the specific antigen of interest on the tissue section.

False Signup and view all the answers

Heat fixation is primarily used for preserving the internal structures of single-cell organisms like bacteria and archaea.

False Signup and view all the answers

Freezing samples for fixation allows for the formation of ice crystals, which helps in preserving the sample's morphology.

False Signup and view all the answers

Chemical fixation aims to preserve the tissues as close to living tissue as possible by making them soluble in the fixative.

False Signup and view all the answers

Fixatives increase the mechanical strength of cells and tissues by altering them on a molecular level.

True Signup and view all the answers

Fixatives are primarily used to enhance the growth of microorganisms in tissue samples.

False Signup and view all the answers

What is the purpose of fixative in cell culture?

To preserve samples by disabling proteolytic enzymes Signup and view all the answers

Which technique is used for fixing single-cell organisms like bacteria and archaea?

Heat Fixation Signup and view all the answers

What does freezing samples for fixation achieve in cell culture?

Allows for detailed results Signup and view all the answers

How does chemical fixation differ from heat fixation in preserving samples?

Chemical Fixation preserves internal structures better Signup and view all the answers

Why is chemical fixation essential for preserving cell structures?

It stabilizes proteins, nucleic acids, and microsubstances, making them insoluble Signup and view all the answers

Match the following factors with their influence on staining dye affinity to the target tissue specimen:

Acidic dye binds with positively charged acidophilic tissue = Specimen geometry: Thick tissue - Less penetration of dye Basic dye binds with negatively charged basophilic tissue = Target concentration: More amount of target tissue - More intense staining Rate of reaction: Short reaction time decreases stain intensity = Rate of stain loss: Too much differentiation removes the stain Signup and view all the answers

Match the following statements about indirect immunocytochemistry:

Amplifies the labeling of the protein of interest = Uses a secondary antibody made in the same species as the primary antibody against the tissue protein One labeled secondary antibody binds multiple primary antibodies = Primary antibody binds directly to the specific antigen of interest on the tissue section Signup and view all the answers

Match the following techniques with their applications in histology:

Enzyme histochemistry = Identify and localize a specific protein within cells Immunohistochemistry = Uses antibodies to detect specific proteins in cells or tissues In situ hybridization = Directly applies nucleic acid sequences to prepared cells and tissue sections Signup and view all the answers

Match the following terms with their relevance to cell culture:

Purpose of fixative = Chemical fixation essential for preserving cell structures Freezing samples for fixation = Allows for formation of ice crystals, helps preserve sample's morphology Signup and view all the answers

Match the following features with their description in immunocytochemistry methods:

Direct immunocytochemistry = Uses labeled primary antibody to bind specifically to tissue protein of interest Indirect immunocytochemistry = Involves applying a secondary antibody labeled with a fluorescent compound or peroxidase to bind to tissue protein of interest Signup and view all the answers

Match the staining component with its function in Leishman stain:

Methylene blue = Stains nuclei and cytoplasm of WBCs Eosin = Stains eosinophilic granules and Hb of red cells Methyl alcohol = Fixes the smear to the slide Hematoxylin = Stains basic part of the cells Signup and view all the answers

Match the blood cell type with its characteristic stain color in Leishman stain:

RBCs = Pink cytoplasm Lymphocytes = Purple cytoplasm Neutrophils = Blue nuclei Basophils = Granules stained blue Signup and view all the answers

Match the staining tip with its recommendation for making a blood smear:

Drop size = Smear length and thickness Speed = Spread length Angle of slide = Smear length determination Stain concentration = Smear color intensity Signup and view all the answers

Match the histological technique with its purpose:

Histochemistry = Identify specific proteins within cells Immunohistochemistry = Detect specific proteins in tissues Enzyme histochemistry = Localize proteins within cells In situ hybridization = Visualize nucleic acids Signup and view all the answers

Match the staining method with its target cellular component:

Leishman stain = Nuclei and cytoplasm of cells Hematoxylin and Eosin stain = Differentiate tissue structures Giemsa stain = Highlight parasites in blood smears Papanicolaou stain = Cervical cytology specimens Signup and view all the answers

Histopathology Tissue Level of Structural Organisation Quiz

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Questions and Answers

Microphotography was already well known before the mid-20th century.

Cells are naturally colorful and opaque, so they do not need to be stained for observation under a microscope.

Camillo Golgi and Santiago Ramon y Cajal shared the Nobel Peace Prize in Physiology or Medicine in 1906 for their work in neuroscience and histology.

The Antoni van Leeuwenhoek microscope was used to observe objects that are very large.

The development of synthetic dyes for textile industry between 1600 and 1800 did not impact the staining techniques used in histology.

The Antoni van Leeuwenhoek microscope was a crude but functional optical instrument used to observe objects that are very small.

Microphotography revolutionized microscopy science before the mid-20th century.

The development of synthetic dyes between 1600 and 1800 did not impact tissue staining techniques in histology.

Most cells are naturally colorful and opaque in their original state, making staining unnecessary for observation under a microscope.

Histology was first recognized as a medical specialty in 1906 by Camillo Golgi and Santiago Ramon y Cajal's pioneering work in neuroscience.

Understanding the normal structure of tissues is crucial for interpreting changes that occur during exams.

Histological techniques allow for the understanding of cell and tissue structure.

Pathology focuses on recognizing the normal appearance of several pathological tissues.

The development of the cellular doctrine by Theodor Schwann was a key 19th-century histological discovery.

Synthetic dyes developed between 1600 and 1800 had no impact on tissue staining techniques in histology.

What is the main focus of histopathology?

Which histological technique is essential for understanding cell and tissue structure?

What did Theodor Schwann propose in 1839 that is significant in histological discoveries?

Which type of tissue makes up the organs of the human body?

In histopathology, what is the primary focus when evaluating cells and tissues?

Enzyme histochemistry is the best approach to identify and localize a specific protein within cells.

In histologic technique, in situ hybridization is used to visualize nucleic acids.

Immunohistochemistry is a technique that uses antibodies to detect specific proteins in cells or tissues.

FISH can be used to detect the t(9;22) translocation in leukemia patients.

Biopsy samples are often analyzed using histochemical techniques to study tissue structure.

Immunohistochemistry can be used to detect and localize specific proteins in cells or tissues.

Prussian blue stain is commonly used to detect iron in tissues during histological examinations.

Biopsy samples are not often analyzed using ancillary tests like molecular studies or flow cytometry.

The source of tissue for cytologic assessment during histological examinations is primarily bone marrow smear aspiration.

In situ hybridization involves directly applying nucleic acid sequences to prepared cells and tissue sections.

What is the study of cells called?

Which component of a cell serves as the structural framework?

What is the fluid component of the cell called?

Which type of tissue is responsible for covering body surfaces and lining body cavities?

Which tissue mainly consists of cells that perform the main function of an organ?

Which immunocytochemistry method uses a labeled secondary antibody?

What type of label is directly attached to the antibody in direct immunocytochemistry?

How is the protein of interest on the tissue section visualized in direct immunocytochemistry?

What is the key feature of indirect immunocytochemistry that contributes to the amplification of protein labeling?

What role does the secondary antibody play in indirect immunocytochemistry?

Direct immunocytochemistry uses a labeled primary antibody to bind specifically to the tissue protein of interest.

Indirect immunocytochemistry amplifies the labeling of the protein of interest due to the ability of one labeled secondary antibody to bind multiple primary antibodies.

Indirect immunocytochemistry uses a secondary antibody made in the same species as the primary antibody against the tissue protein.

Direct immunocytochemistry involves applying a secondary antibody labeled with a fluorescent compound or peroxidase to bind to the tissue protein of interest.

In indirect immunocytochemistry, the primary antibody binds directly to the specific antigen of interest on the tissue section.

Heat fixation is primarily used for preserving the internal structures of single-cell organisms like bacteria and archaea.

Freezing samples for fixation allows for the formation of ice crystals, which helps in preserving the sample's morphology.

Chemical fixation aims to preserve the tissues as close to living tissue as possible by making them soluble in the fixative.

Fixatives increase the mechanical strength of cells and tissues by altering them on a molecular level.

Fixatives are primarily used to enhance the growth of microorganisms in tissue samples.

What is the purpose of fixative in cell culture?

Which technique is used for fixing single-cell organisms like bacteria and archaea?

What does freezing samples for fixation achieve in cell culture?

How does chemical fixation differ from heat fixation in preserving samples?

Why is chemical fixation essential for preserving cell structures?

Match the following factors with their influence on staining dye affinity to the target tissue specimen:

Match the following statements about indirect immunocytochemistry:

Match the following techniques with their applications in histology:

Match the following terms with their relevance to cell culture:

Match the following features with their description in immunocytochemistry methods:

Match the staining component with its function in Leishman stain:

Match the blood cell type with its characteristic stain color in Leishman stain:

Match the staining tip with its recommendation for making a blood smear:

Match the histological technique with its purpose:

Match the staining method with its target cellular component:

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