Histopathological Techniques: Introduction

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What is the main purpose of a diagnostic endoscopy biopsy?

To cauterize tumors to prevent further growth

To surgically remove tumors in their entirety

To inject medication directly into tumors

To directly visualize an abnormal area and obtain tissue samples for examination (correct)

Which technique involves the use of biopsy forceps to sample skin rashes and small masses?

Punch biopsy (correct)

Brush biopsies

Core needle biopsy

Curettage biopsy

What does the histopathological technique 'Fixation' aim to do?

Preserve the microscopic anatomy of the tissue in its natural state (correct)

Increase the size of tumor samples for easier examination

Magnify the cellular features of the lesions

Apply dye to highlight cellular structures

In which procedure is a wide bore biopsy needle used for a percutaneous approach to internal structures?

Core needle biopsy Signup and view all the answers

When is curettage biopsy usually done?

For the diagnosis of endometrial disease Signup and view all the answers

What is the main function of brush biopsies?

To evaluate superficial cellular features of lesions Signup and view all the answers

What is the difference between histology and histopathology?

Histology focuses on microscopic anatomy, while histopathology focuses on the study of disease in a tissue section. Signup and view all the answers

What is the purpose of an excisional biopsy?

To remove a whole organ or lump for examination. Signup and view all the answers

When is an incisional biopsy typically performed?

Prior to a major surgical procedure. Signup and view all the answers

What are the different methods typically used for taking biopsy?

Punch biopsy, curettage biopsy, and brush biopsies. Signup and view all the answers

What is the main purpose of an autopsy?

To examine dead body tissue in forensic medicine. Signup and view all the answers

Who is responsible for taking a biopsy according to the given text?

The physician. Signup and view all the answers

What is the main purpose of solidification in histopathological techniques?

Prevent autolysis and bacterial decomposition Signup and view all the answers

Which classification of fixatives is mainly used to preserve intracellular structures or inclusions?

Cold acetone Signup and view all the answers

What is autolysis in histopathological techniques?

Lysis or dissolution of cells by enzymatic action Signup and view all the answers

Which property is NOT associated with an ideal fixative according to the text?

Make cellular components soluble to reagents Signup and view all the answers

What is putrefaction in histopathological terms?

Breakdown of tissue by bacterial action Signup and view all the answers

Which is a factor that affects fixation time in histopathological techniques?

Temperature of the fixative Signup and view all the answers

What is the purpose of fixation in histopathological technique?

To prevent autolysis and putrefaction Signup and view all the answers

What is the primary role of fixatives in the fixation process?

Cause denaturation of proteins Signup and view all the answers

Which of the following is NOT an effect or aim of fixation?

Causing autolysis Signup and view all the answers

What is the purpose of dehydration in biopsy processes?

To prepare tissue for embedding Signup and view all the answers

Which process in the hard biopsy technique is responsible for removing calcium salts?

Decalcification Signup and view all the answers

What key change occurs in tissues during fixation to make manipulation easier?

Precipitation of proteins Signup and view all the answers

What is the recommended pH range for the best fixation?

pH 6-8 Signup and view all the answers

Why is it advised to be cautious with high temperatures during fixation?

Heat speeds up protein coagulation and autolysis Signup and view all the answers

Which fixatives are considered the best penetrators?

Alcohol and formalin Signup and view all the answers

How much tissue penetration is typically achieved by fixatives in a 24-hour period?

2-3 mm of solid tissue or 0.5 cm of porous tissue Signup and view all the answers

What is the ideal fixative to tissue ratio for fixation?

20:1 Signup and view all the answers