Histopathological Techniques: Introduction

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Questions and Answers

What is the main purpose of a diagnostic endoscopy biopsy?

  • To cauterize tumors to prevent further growth
  • To surgically remove tumors in their entirety
  • To inject medication directly into tumors
  • To directly visualize an abnormal area and obtain tissue samples for examination (correct)

Which technique involves the use of biopsy forceps to sample skin rashes and small masses?

  • Punch biopsy (correct)
  • Brush biopsies
  • Core needle biopsy
  • Curettage biopsy

What does the histopathological technique 'Fixation' aim to do?

  • Preserve the microscopic anatomy of the tissue in its natural state (correct)
  • Increase the size of tumor samples for easier examination
  • Magnify the cellular features of the lesions
  • Apply dye to highlight cellular structures

In which procedure is a wide bore biopsy needle used for a percutaneous approach to internal structures?

<p>Core needle biopsy (B)</p> Signup and view all the answers

When is curettage biopsy usually done?

<p>For the diagnosis of endometrial disease (A)</p> Signup and view all the answers

What is the main function of brush biopsies?

<p>To evaluate superficial cellular features of lesions (B)</p> Signup and view all the answers

What is the difference between histology and histopathology?

<p>Histology focuses on microscopic anatomy, while histopathology focuses on the study of disease in a tissue section. (D)</p> Signup and view all the answers

What is the purpose of an excisional biopsy?

<p>To remove a whole organ or lump for examination. (C)</p> Signup and view all the answers

When is an incisional biopsy typically performed?

<p>Prior to a major surgical procedure. (A)</p> Signup and view all the answers

What are the different methods typically used for taking biopsy?

<p>Punch biopsy, curettage biopsy, and brush biopsies. (A)</p> Signup and view all the answers

What is the main purpose of an autopsy?

<p>To examine dead body tissue in forensic medicine. (B)</p> Signup and view all the answers

Who is responsible for taking a biopsy according to the given text?

<p>The physician. (B)</p> Signup and view all the answers

What is the main purpose of solidification in histopathological techniques?

<p>Prevent autolysis and bacterial decomposition (D)</p> Signup and view all the answers

Which classification of fixatives is mainly used to preserve intracellular structures or inclusions?

<p>Cold acetone (A)</p> Signup and view all the answers

What is autolysis in histopathological techniques?

<p>Lysis or dissolution of cells by enzymatic action (A)</p> Signup and view all the answers

Which property is NOT associated with an ideal fixative according to the text?

<p>Make cellular components soluble to reagents (B)</p> Signup and view all the answers

What is putrefaction in histopathological terms?

<p>Breakdown of tissue by bacterial action (A)</p> Signup and view all the answers

Which is a factor that affects fixation time in histopathological techniques?

<p>Temperature of the fixative (B)</p> Signup and view all the answers

What is the purpose of fixation in histopathological technique?

<p>To prevent autolysis and putrefaction (A)</p> Signup and view all the answers

What is the primary role of fixatives in the fixation process?

<p>Cause denaturation of proteins (D)</p> Signup and view all the answers

Which of the following is NOT an effect or aim of fixation?

<p>Causing autolysis (C)</p> Signup and view all the answers

What is the purpose of dehydration in biopsy processes?

<p>To prepare tissue for embedding (B)</p> Signup and view all the answers

Which process in the hard biopsy technique is responsible for removing calcium salts?

<p>Decalcification (D)</p> Signup and view all the answers

What key change occurs in tissues during fixation to make manipulation easier?

<p>Precipitation of proteins (B)</p> Signup and view all the answers

What is the recommended pH range for the best fixation?

<p>pH 6-8 (D)</p> Signup and view all the answers

Why is it advised to be cautious with high temperatures during fixation?

<p>Heat speeds up protein coagulation and autolysis (A)</p> Signup and view all the answers

Which fixatives are considered the best penetrators?

<p>Alcohol and formalin (B)</p> Signup and view all the answers

How much tissue penetration is typically achieved by fixatives in a 24-hour period?

<p>2-3 mm of solid tissue or 0.5 cm of porous tissue (B)</p> Signup and view all the answers

What is the ideal fixative to tissue ratio for fixation?

<p>20:1 (D)</p> Signup and view all the answers

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