25 Questions
Which substance is commonly used as a rapid clearing agent?
Xylene
What is a substance that is miscible in both the impregnating medium and mounting medium called?
Clearing agent
Which substance is both a dehydrating agent and a clearing agent?
Anhydrous copper sulfate
Which clearing agent is recommended for CNS tissues and cytological studies?
Clove oil
Which substance is not recommended for routine purposes due to its flammability and volatility as a dehydrating agent?
Acetone
What does the term 'clearing' refer to in tissue processing?
Making the tissue translucent by replacing alcohol or dehydrating agent
What is the function of clearance angle in knife sharpening?
It acquires an even edge after final polishing
What is the primary function of a microtome in laboratory procedures?
To cut tissues into thin sections
Which type of microtome is considered the simplest among the various microtome types?
Rocking microtome
What is the importance of orientation in microtomy procedures?
To arrange tissue in precise positions before cutting
Which angle defines the angle between the block face and the upper facet of the knife in sectioning?
Cutting angle
What does the term 'trimming' refer to in tissue processing procedures?
Removing excess wax post-embedding
In which type of microtome is a movable exposed knife considered potentially dangerous?
Sliding microtome
'Wedge angle' in knife sharpening corresponds to what aspect of blade maintenance?
'Wedge angle' involves edges of a wedge knife
'Sectioning' refers to what specific action in laboratory procedures?
Cutting tissues into thin slices with a microtome
What does a 'peel-away' disposable embedding mold primarily facilitate?
Removing trimmed tissue sections efficiently
What is the recommended ratio of volume of decalcifying agent to tissue?
20 to 1
Which acid is not recommended for urgent biopsies due to its dual function as a tissue softener and decalcifying agent?
Perenyi's fluid
What acid is recommended for routine decalcification of postmortem research tissues and is also a fixative?
Formic acid
Which decalcifying agent is most rapid and recommended for urgent biopsies?
Phloroglucin-nitric acid
Which chelating agent is recommended only for detailed microscopic studies?
EDTA (Versene)
Which fluid is moderately rapid in decalcification and recommended for teeth and small pieces of bones?
Von Ebner's fluid
'Electrical ionization (electrophoresis)' is used to remove calcium ions from the decalcifying solution due to its attraction to:
Positive electrode
'Zenker's fluid' and 'Helly's fluid' are commonly used as fixatives in __________.
'Postmortem research tissues'
'Calcium Oxalate Test' is a __________ method to measure the extent of decalcification.
'Chemical Method'
Learn about the decalcification process in histopathology, including the removal of calcium or lime salts from tissues. Understand when and how decalcification should be performed to ensure optimal tissue preparation for examination.
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