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Excisional Biopsy Removes whole area in question, entire lesion is sent for ______
biopsy
Histopathologic Techniques - MTAP 1 Finals Histology vs. Histopathology Equipment and Apparatus Cryostat Apparatus used in fresh tissue microtomy (frozen sections) Left ON even when not in use Optimum working temp: -18-20 Temperature situations Þ Tissue < Knife o Compression displaced structures Þ Tissue > Knife o Melts tissue, sticky & distorted mass Þ Tissue >> Knife o Resistant to cutting, brittle, ______
fragments
Incisional Biopsy Removal of a small piece of a lesion/tumor, which is sent for diagnosis before final removal. Þ If it is cancerous, it is then removed via ______
surgery
Types of Samples Incisional Biopsy Removal of a small piece of a lesion/tumor, which is sent for diagnosis before final ______
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Fine Needle Aspiration Simplest, least invasive test, which uses the smallest to remove cells from the area of abnormality Þ It is not always adequate for ______
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Explain the purpose of a cryostat apparatus in histopathologic techniques and its optimum working temperature range.
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Distinguish between histology and histopathology.
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What are the two types of samples commonly used in biopsies and how do they differ?
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Describe the process and purpose of fine needle aspiration in histopathologic testing.
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Explain the potential consequences of tissue being too close or too far from the knife during microtomy using a cryostat apparatus.
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Study Notes
Histopathologic Techniques
- Cryostat is an apparatus used in fresh tissue microtomy (frozen sections) and is left on even when not in use.
- Optimum working temperature of Cryostat is -18-20°C.
- Temperature situations affect the quality of tissue cutting:
- Tissue < Knife: Compression displaced structures
- Tissue > Knife: Melts tissue, sticky & distorted mass
- Tissue >> Knife: Resistant to cutting, brittle, fragments
Histology vs. Histopathology
- Histology is the microscopic study of normal tissues of the body.
- Histopathology is the branch of pathology dealing with diseased tissues.
Types of Samples
- Incisional Biopsy: Removal of a small piece of a lesion/tumor, which is sent for diagnosis before final removal.
- Excisional Biopsy: Removes whole area in question, entire lesion is sent for biopsy.
- Fine Needle Aspiration: Simplest, least invasive test, which uses the smallest needle to remove cells from the area of abnormality.
- Note: Fine Needle Aspiration is not always adequate for diagnosis, depending on the situation.
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Description
Test your knowledge of histopathologic techniques with this quiz on equipment and apparatus used in histology and histopathology. From cryostat apparatus to temperature considerations, this quiz will help you review essential concepts for MTAP 1 Finals.
