Introduction to Histology Quiz

The study of tissues in the body and how they are arranged to form organs.

A process that preserves tissue structure for microscopic examination, usually using formalin.

The removal of water from tissue using increasing alcohol concentrations to prevent decay.

The process of using dyes to highlight specific structures in tissue.

The removal of alcohol from tissue using a solvent like xylene, before embedding.

The process of replacing the clearing agent with a substance that solidifies, like wax or resin, to create a firm block for slicing.

A specialized instrument used to cut thin slices of tissue for microscopic examination.

The thin, ribbon-like slices of tissue produced by a microtome.

A process in microscopy where a tissue sample is embedded in a supporting medium, usually paraffin wax, to make it easier to cut into thin sections for viewing under a microscope.

The ability of a microscope lens or optical system to produce separate images of closely positioned objects.

A basic dye used in microscopy, often in combination with eosin, to stain cell nuclei blue or purple.

An acidic dye used in microscopy, often in combination with hematoxylin, to stain cytoplasm pink or red.

A type of microscopy that utilizes ordinary visible light that passes through the specimen, commonly used with stained preparations.

A type of microscopy where a specimen is illuminated from the side, allowing scattered light to be collected, making unstained objects appear bright against a dark background.

A type of microscopy that uses polarized light to enhance contrast and reveal structures that are invisible in ordinary light microscopy.

A type of microscopy that uses a fluorescent dye to label specific structures, allowing for the visualization of those structures.

A type of microscopy used to visualize thin, spiral-shaped bacteria like those in syphilis.

A technique that uses polarized light to enhance visualization of structures with birefringence (ability to split light into two rays).

A type of microscopy that combines components of a light optical microscope with a scanning system for optical dissection.

Confocal Microscopy uses a small, high-intensity light point, often from a laser, and a plate with a pinhole aperture to improve image clarity.

In electron microscopy, tissue components interact with beams of electrons to create images.

Electron microscopy offers a 1000-fold resolution increase compared to light microscopy due to the significantly shorter wavelength of electrons.

Electron microscopy has two main types: Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM).

A type of microscopy that uses electrons to create images of tiny structures, allowing you to see details down to 3 nanometers.

A technique that uses very high magnification (up to 400,000 times) to examine the fine details of cells and tissues.

The ability of certain materials, like actin filaments and crystals, to rotate the direction of polarized light.

A special method for preparing samples for TEM, where the tissue is frozen and then cracked open to reveal the inner structures of membranes.

A method of localizing messenger RNA (mRNA) or DNA by hybridizing the sequence of interest to a complementary strand of a nucleotide probe.

A method for localizing cellular structures using a specific enzymatic activity present in those structures.

A highly specific interaction between molecules is that between an antigen and its antibody.

A specific type of in situ hybridization where a fluorescently labeled complementary probe is used.

A technique used to visualize chromosomes and detect abnormalities like deletions, duplications, or translocations.

A type of microscopy that uses fluorescent antibodies to label and visualize specific targets within a sample.

Immunofluorescence where fluorescently labeled primary antibodies bind directly to the target antigen.

Immunofluorescence where fluorescently labeled secondary antibodies bind to primary antibodies, which are already bound to the target antigen.

A type of microscopy that uses a focused electron beam to scan the surface of a specimen.

A technique used to identify and locate specific DNA sequences within a sample.

A component in an electron microscope that emits electrons.

A component in an electron microscope that attracts emitted electrons.

A lens in the electron microscope that focuses the electron beams onto the specimen.