20 Questions
Trimming is the process of removing excess ______ after embedding.
wax
Four-sided prism or Truncated Pyramid shape is used to ensure at least 2mm of ______ should surround the tissue block.
wax
Recommended Thickness of Tissue Sections for routine histologic procedure is 4 - 5μ, for Frozen Sections is 10 - 15μ, and for Electron Microscopy is ______.
0.5μ
Rocking Microtome, also known as Cambridge Rocking Microtome, is used for large blocks of paraffin embedded tissues and was invented by Paldwell-Trefall on ______.
1881
The sliding microtome is used for celloidin embedded tissue sections. Knife = ______; Tissue block = moving
stationary
The freezing microtome is also known as Cryostats/Cold ______. Temperature should be maintained at - 5 to -30°C
Microtome
The VIBROTOME is used for unfixed, unfrozen specimen section for ______ demonstration
enzyme
ULTRATHIN MICROTOME is for cutting sections for ______ Microscopy
Electron
The sliding microtome is used for celloidin embedded tissue sections. Knife = ______; Tissue block = moving
stationary
The sliding microtome is used for celloidin embedded tissue sections. Knife = moving; Tissue block = ______
stationary
ULTRATHIN MICROTOME is for cutting sections for ______ Microscopy
Electron
The freezing microtome is also known as Cryostats/Cold ______. Temperature should be maintained at - 5 to -30°C
Knife
The sliding microtome is used for celloidin embedded tissue sections. Knife = moving; Tissue block = ______
stationary
Recommended Thickness of Tissue Sections for routine histologic procedure is 4 - 5μ, for Frozen Sections is 10 - 15μ, and for Electron Microscopy is ______.
0.5μ
The sliding microtome is used for celloidin embedded tissue sections. Knife = ______; Tissue block = moving
stationary
The freezing microtome is also known as Cryostats/Cold ______. Temperature should be maintained at - 5 to -30°C
Microtome
The VIBROTOME is used for unfixed, unfrozen specimen section for ______ demonstration
enzyme
ULTRATHIN MICROTOME is for cutting sections for ______ Microscopy
Electron
Trimming is the process of removing excess ______ after embedding.
tissue
Rocking Microtome, also known as Cambridge Rocking Microtome, is used for large blocks of paraffin embedded tissues and was invented by Paldwell-Trefall on ______.
Four-sided
Study Notes
Trimming and Microtome
- Trimming is the process of removing excess wax after embedding.
- A four-sided prism or Truncated Pyramid shape is used to ensure at least 2mm of wax surrounds the tissue block.
Thickness of Tissue Sections
- Recommended thickness for routine histologic procedure is 4-5μ.
- Recommended thickness for Frozen Sections is 10-15μ.
- Recommended thickness for Electron Microscopy is 60-90μ.
Microtome Types
- Rocking Microtome (also known as Cambridge Rocking Microtome) is used for large blocks of paraffin embedded tissues and was invented by Paldwell-Trefall in 1885.
- Sliding Microtome is used for celloidin embedded tissue sections, where the knife is stationary and the tissue block is moving.
- Freezing Microtome is also known as Cryostats/Cold Plate, and the temperature should be maintained at - 5 to -30°C.
- Vibrotome is used for unfixed, unfrozen specimen section for demonstration of delicate structures.
- Ultrathin Microtome is used for cutting sections for Electron Microscopy.
Test your knowledge on the process of trimming and sectioning tissue blocks for histological studies. Learn about the tools used, recommended thickness, and post-sectioning procedures such as staining.
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