15 Questions
Why is tissue processing necessary in histology?
To impregnate the tissue with a solid medium for easier storage
What is the purpose of a numbering system for each specimen in laboratories?
To avoid confusion regarding duplication of names and diagnoses
What advantage do fixed impregnated tissues have over frozen tissues?
Easier storage and reproducibility of sections
Why should labeling not be done using ordinary ink?
It gets dissolved in the reagent used during processing
What type of label is satisfactory for proper tissue labeling?
Thin white card with a soft lead pencil
What is the purpose of tissue processing in histology?
To remove tissue water and replace it with an embedding medium
What is the role of the tissue processing baskets in histology?
To enable transfer of tissue and labels from reagent to reagent
What is the most satisfactory embedding medium used in routine histology?
Paraffin wax
What is the purpose of removing tissue water in the tissue processing procedure?
To allow the embedding medium to thoroughly permeate the tissue
What is the purpose of the clearing step in tissue processing?
To make the tissue miscible with paraffin wax
What is the function of the Tissue Tek system in histology?
To retain the tissue identity as a permanent record during sectioning and storage
How does efficient agitation impact tissue processing?
All of the above
What factor influences the rate of penetration in tissue impregnation?
Viscosity
What is the purpose of the fixation step in tissue processing?
To preserve the structure of the tissue
How does heat impact penetration rate in tissue impregnation?
Increases the rate of penetration
Explore the techniques and processes involved in treating tissues to render them firm yet elastic for cutting tissue sections. Learn about impregnating tissues into a solid medium and producing sections using cryostat or freezing microtome on frozen tissues.
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