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Tissue Processing and Sectioning Techniques

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Tissue Processing and Sectioning Techniques

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Questions and Answers

Why is tissue processing necessary in histology?

To impregnate the tissue with a solid medium for easier storage (correct)
To freeze the tissue for easier cutting on a microtome
To ensure proper labeling of the tissue
To make the tissue sections more elastic

What is the purpose of a numbering system for each specimen in laboratories?

To make the tissue sections more firm
To speed up the tissue processing time
To ensure proper labeling using ordinary ink
To avoid confusion regarding duplication of names and diagnoses (correct)

What advantage do fixed impregnated tissues have over frozen tissues?

Less chance of contamination
Easier storage and reproducibility of sections (correct)
More elastic sections
Quicker processing time

Why should labeling not be done using ordinary ink?

It gets dissolved in the reagent used during processing (D) Signup and view all the answers

What type of label is satisfactory for proper tissue labeling?

Thin white card with a soft lead pencil (D) Signup and view all the answers

What is the purpose of tissue processing in histology?

To remove tissue water and replace it with an embedding medium (B) Signup and view all the answers

What is the role of the tissue processing baskets in histology?

To enable transfer of tissue and labels from reagent to reagent (C) Signup and view all the answers

What is the most satisfactory embedding medium used in routine histology?

Paraffin wax (D) Signup and view all the answers

What is the purpose of removing tissue water in the tissue processing procedure?

To allow the embedding medium to thoroughly permeate the tissue (C) Signup and view all the answers

What is the purpose of the clearing step in tissue processing?

To make the tissue miscible with paraffin wax (D) Signup and view all the answers

What is the function of the Tissue Tek system in histology?

To retain the tissue identity as a permanent record during sectioning and storage (D) Signup and view all the answers

How does efficient agitation impact tissue processing?

All of the above (D) Signup and view all the answers

What factor influences the rate of penetration in tissue impregnation?

Viscosity (A) Signup and view all the answers

What is the purpose of the fixation step in tissue processing?

To preserve the structure of the tissue (C) Signup and view all the answers

How does heat impact penetration rate in tissue impregnation?

Increases the rate of penetration (D) Signup and view all the answers