Histology Fundamentals Quiz

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Questions and Answers

Why is the preparation process often not feasible?

Because it can cause slight structural distortions and the removal of cellular lipid. (correct)
Because it always results in the complete loss of cellular structure.
Because the process only works on certain cell types.
Because it requires extensive training to perform.

What is the relationship between tissues and organs?

Tissues and organs are interchangeable in the body.
Tissues and organs are developed independently of each other.
Tissues are formed by a combination of different types of organs.
Organs are formed by an orderly combination of different types of tissues. (correct)

What role do advances in other scientific fields play in the study of histology?

They are essential for a deeper understanding of histology. (correct)
They are primarily used to make microscopic tools.
They are only useful for identifying new types of cells.
They are unnecessary for studying histology.

What is the main reason for using fixation in tissue preparation?

To preserve tissue structure and prevent degradation. (D) Signup and view all the answers

Which of the followings best describes why histology depends on microscopes?

To view the small size of cells and matrix components. (B) Signup and view all the answers

What is the primary purpose of fixation in tissue preparation?

To cross-link proteins and inactivate degradative enzymes (D) Signup and view all the answers

In the process of tissue dehydration, what is the final solution used?

A 100% alcohol solution (B) Signup and view all the answers

What is the purpose of 'clearing' in histological tissue processing?

To replace alcohol with a solution that is miscible with paraffin (B) Signup and view all the answers

What is the temperature range typically used in the process of tissue infiltration with paraffin?

52°-60°C (D) Signup and view all the answers

What instrument is used to cut paraffin-embedded tissue into thin sections for light microscopy?

A microtome (A) Signup and view all the answers

What material is used to embed tissue for Transmission Electron Microscopy (TEM)?

Epoxy Resins (D) Signup and view all the answers

In tissue sectioning using a microtome, what is the typical range of thickness advanced by each turn of the drive wheel?

1 to 10 μm (C) Signup and view all the answers

After dehydration, what type of solvents are used?

Organic solvents (A) Signup and view all the answers

What is the purpose of the 'clearing' step in tissue processing?

To replace the dehydrating agent with a solvent miscible with both dehydrating agent and embedding medium, making the tissue translucent. (B) Signup and view all the answers

Which embedding medium requires lower temperatures, thus avoiding tissue distortion?

Resin (A) Signup and view all the answers

What is the primary function of a microtome in tissue preparation?

To section embedded tissues into thin slices. (B) Signup and view all the answers

What type of tissue components are primarily stained by basic dyes?

Anionic components with a net negative charge such as DNA and RNA. (D) Signup and view all the answers

Which of the following best describes the staining characteristics of hematoxylin?

It behaves as a basic dye, staining basophilic tissue components. (C) Signup and view all the answers

Which cellular structures or tissues are stained pink by eosin in H&E staining?

Mitochondria, secretory granules, and collagen. (A) Signup and view all the answers

What is the primary purpose of embedding tissues before sectioning?

To provide the tissue with a firm consistency for thin sectioning. (A) Signup and view all the answers

Which of the following describes the process of dehydrating a fixed tissue?

Gradual water extraction using a series of increasing ethanol concentrations. (C) Signup and view all the answers

What is stained purple or dark blue by hematoxylin in H&E staining?

DNA in the cell nucleus, RNA-rich portions of the cytoplasm, and cartilage matrix. (D) Signup and view all the answers

What is the typical thickness of paraffin sections for light microscopy?

3-10 μm (C) Signup and view all the answers

Why are tissue sections typically stained after being prepared?

To render colorless tissue components visible and distinguishable. (A) Signup and view all the answers

What type of chemical interaction is primarily responsible for staining tissues?

Electrostatic (salt) linkages between dye molecules and tissue. (B) Signup and view all the answers

Which type of microscopy primarily uses paraffin as an embedding material?

Light microscopy. (C) Signup and view all the answers

Which embedding material is suitable for use in both light and electron microscopy?

Plastic resins. (A) Signup and view all the answers

What role does ethanol concentration play in tissue dehydration?

The concentration is gradually increased to ensure gradual water extraction. (A) Signup and view all the answers

What is the main purpose of fixing tissue before processing?

To preserve tissue structure and prevent degradation. (C) Signup and view all the answers

Why is it necessary to cut tissues into small fragments before fixation?

To facilitate the rapid and complete diffusion of the fixative through the tissue. (C) Signup and view all the answers

Which of the following is a fixative commonly used for light microscopy?

Formalin. (C) Signup and view all the answers

What is the function of osmium tetroxide in electron microscopy?

To provide contrast by staining lipids and preserving cellular membranes. (A) Signup and view all the answers

What is the purpose of embedding tissue in paraffin?

To provide support for the tissue and create a block for sectioning. (B) Signup and view all the answers

What is the key difference between sectioning for light microscopy and transmission electron microscopy (TEM), described in the text?

Light microscopy sections are thicker than TEM sections. (B) Signup and view all the answers

What is the purpose of trimming a paraffin block before sectioning on a microtome?

To expose the tissue for sectioning. (A) Signup and view all the answers

In vascular perfusion, how are fixatives introduced into the body?

Via the blood vessels. (D) Signup and view all the answers

In the context of the provided micrographs, what is the primary purpose of using the PAS reaction?

To highlight regions rich in oligosaccharides and polysaccharides. (A) Signup and view all the answers

What does the purple staining in the H&E stained micrograph (a) primarily indicate?

Basophilic cell nuclei. (B) Signup and view all the answers

What is the role of hematoxylin in the PAS-stained tissue?

To stain cell nuclei as a counter stain. (D) Signup and view all the answers

According to the passage, what is the final step before observation of a slide using a microscope?

Applying a glass coverslip to the slide. (D) Signup and view all the answers

What is the approximate total magnification of tissue in image b given an objective lens of 10x and an ocular lens of 30x?

300x (A) Signup and view all the answers

What cellular feature is primarily highlighted by staining with the PAS reaction?

Oligosaccharides and polysaccharides. (A) Signup and view all the answers

Based on the context, which cell type is most prominently associated with the staining pattern observed in the PAS-stained micrograph?

Goblet cells (D) Signup and view all the answers

Why are tissue samples mounted with a glass coverslip before microscopic observation?

To protect the sample and provide a flat viewing surface. (D) Signup and view all the answers

Flashcards

Tissue Preparation

The process of preparing biological tissue for examination under a microscope.

Fixation

A step in tissue preparation that preserves structures and prevents degradation by enzymes.