Cytoplasmic Fixatives in Histology Quiz and Flashcards

Podcast Beta

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the fixative of choice for tissue photography?

B5 FIXATIVE

ZENKER’S FLUID (correct)

Lead Fixatives

MERCURIC CHLORIDE FIXATIVES

Which fixative may produce black granular deposits and requires dezenkerization to remove them?

Chromate Fixatives

Mercuric Chloride Fixatives (correct)

Lead Fixatives

Metalic Fixatives

What is the function of ZENKER’S FLUID fixative?

Skin Tumor Biopsy

Pituitary Gland, Blood Containing Organs

Bone Marrow Biopsies

Liver, Spleen, Connective Tissue Fiber, Nuclei (correct)

Which fixative is most commonly described as a strong oxidizing reagent?

Chromate Fixatives Signup and view all the answers

What is the process of removing black granular deposits observed when using mercuric chloride fixatives?

Dezenkerization Signup and view all the answers

What is the main purpose of fixatives in tissue preservation?

Preventing autolysis Signup and view all the answers

What is the optimal pH range for fixation according to the text?

6-8 Signup and view all the answers

What is the typical osmolality range for fixation solutions?

400-500 mOsm Signup and view all the answers

At what rate does the fixative typically penetrate tissues?

1 mm/hour Signup and view all the answers

What is the working temperature range for routine histopathology fixation?

20-25 degree Celsius Signup and view all the answers

Where should the fixation temperature be maintained for TB patients?

-100 degree Celsius Signup and view all the answers

Which fixative is commonly used for the chrome-osmium demonstration of acetic acid fixative structures?

Fleming’s Solution without Acetic Acid Signup and view all the answers

Which fixative is recommended for the preservation of nuclear structures and cytoplasmic mitochondria?

Fleming’s Solution without Acetic Acid Signup and view all the answers

What is the function of Trichloroacetic Acid (TCA) as a fixative?

Decalcifying Agent Signup and view all the answers

Which technique involves the use of frozen tissue sections for fixation?

Microwave Technique Signup and view all the answers

What is the optimum rapid fixation temperature as mentioned in the text?

45-55 Degree Celsius Signup and view all the answers

What is the primary goal of the fixation process in histopathology?

To preserve the morphologic and chemical integrity of the cell Signup and view all the answers

Why is fixation considered a critical step in routine histopathology?

Because it allows preservation of tissues and cells identical to that observed during life Signup and view all the answers

What are fixatives in the context of histopathology fixation?

Chemical reagents used in the fixation process Signup and view all the answers

When is fixation typically performed in histopathology?

Immediately after removing the tissue from the body Signup and view all the answers

Which of the following best describes the secondary goal of fixation in histopathology?

To harden and preserve the tissue for further handling Signup and view all the answers

Why will improperly fixed tissues cause problems in routine histopathology?

Because it will cause issues in proceeding to following steps Signup and view all the answers

What is the special consideration for fixation of hollow organs like intestines and stomach?

Using cotton inside for faster fixation Signup and view all the answers

Which fixative becomes a part of the tissue due to its mechanism?

Formalin Signup and view all the answers

What is the special consideration in fixation for hard tissues like nails and bones?

Immersing them in tissue softeners Signup and view all the answers

What is the purpose of cytoplasmic fixatives?

Preserve membrane-bound organelles Signup and view all the answers

What is the classification of fixatives based on composition if they consist of two or more fixatives?

Compound Signup and view all the answers

Which fixative is appropriate when determining the presence of mucopolysaccharides in cells or tissues?

Histochemical fixatives Signup and view all the answers

Study Notes

Fixatives in Histopathology

The fixative of choice for tissue photography is not specified in the text.

Fixative Characteristics

Mercuric chloride fixative may produce black granular deposits, which require dezenkerization to remove.
ZENKER'S FLUID fixative is used to preserve nuclear structures and cytoplasmic mitochondria.
Chromic acid fixative is commonly used for the chrome-osmium demonstration of acetic acid fixative structures.
Osmium tetroxide is a strong oxidizing reagent.
Trichloroacetic Acid (TCA) is used as a fixative for tissue preservation.

Fixation Process

Dezenkerization is the process of removing black granular deposits observed when using mercuric chloride fixatives.
The primary goal of the fixation process in histopathology is to preserve tissue morphology and prevent autolysis.
Fixation is considered a critical step in routine histopathology because it allows for the preservation of tissue structure and prevents artifacts.
The optimal pH range for fixation is not specified in the text.
The typical osmolality range for fixation solutions is not specified in the text.
Fixatives typically penetrate tissues at a rate of 1-2 mm per hour.
The working temperature range for routine histopathology fixation is 4-20°C.
For TB patients, the fixation temperature should be maintained at 4°C.

Special Considerations

Fixation of hollow organs like intestines and stomach requires special consideration, as they may be prone to collapse.
Fixation of hard tissues like nails and bones requires special consideration, as they may be difficult to fix.
Cytoplasmic fixatives are used to preserve the cytoplasm of cells.
Formalin becomes a part of the tissue due to its mechanism.
The classification of fixatives based on composition is that fixatives consisting of two or more fixatives are classified as compound fixatives.
For determining the presence of mucopolysaccharides in cells or tissues, cetylpyridinium chloride is the appropriate fixative.

Techniques

Cryofixation is a technique that involves the use of frozen tissue sections for fixation.
The optimum rapid fixation temperature is -30°C to -40°C.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Test your knowledge on the actions of histology fixatives in preserving tissue by stopping cellular activities, preventing autolysis, and stabilizing proteins. Learn about the importance of fixatives in easier handling, resistance to damage, and bacterial decomposition.

Histology Fixatives and Their Actions Quiz