Recent Lessons

Show all results for ""

Feature Overview

Ace your exams with our all-in-one platform for creating and sharing quizzes and tests.

Quizzes

Create quizzes and tests automatically from your content using AI.

Flashcards

Automatically turn your notes into digital flashcards.

Share, Export & Embed

Share with classmates or export to Excel and your learning management system.

Stats & Reporting

Auto-grading quizzes and tests with detailed stats and reports.

Mobile Apps

The smarter way to study – wherever you are.

Pricing

Search...

18 Questions

2 Views

Tissue Fixation Considerations

Choose a study mode

Play Quiz

Study Flashcards

Spaced Repetition

Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the recommended duration of formalin fixation?

2 to 6 hours (correct)

4 to 8 hours

1 to 3 hours

6 to 10 hours

What is the ideal volume of fixative in relation to tissue volume for maximum effectiveness?

2 times the tissue volume

20 times the tissue volume (correct)

Equal to tissue volume

Less than tissue volume

What are the characteristics of a good fixative based on the text?

Cheap, stable, safe to handle (correct)

Slow penetration, isotonic, inhibits staining procedures

Expensive, unstable, unsafe

Kills cells slowly, causes maximum shrinkage, enhances bacterial decomposition

What effect do fixatives have on tissues based on the text?

Hardens tissues for easy handling Signup and view all the answers

What should be done if autopsy materials cannot be fixed immediately?

Place in a mortuary refrigerator at 4°C Signup and view all the answers

What happens if fixation is prolonged according to the text?

Tissue hardens and shrinks Signup and view all the answers

What is the main goal of fixation in histotechnology?

Preserve morphologic and chemical integrity of cells Signup and view all the answers

Which type of fixation involves the fixing agent becoming part of the tissue by forming cross links?

Additive fixation Signup and view all the answers

What is the ideal pH range for fixation in histotechnology?

pH 6 to 8 Signup and view all the answers

In what temperature range are tissues processed for electron microscopy fixation?

40°C Signup and view all the answers

What effect does hypertonic fixative have on cells during fixation?

Cell shrinkage Signup and view all the answers

What is the recommended concentration of glutaraldehyde for immuno electron microscopy fixation?

0.5% Signup and view all the answers

What is the purpose of placing a primarily fixed tissue in a 2.5 - 3% potassium dichromate solution for 24 hours?

To act as a mordant for better staining Signup and view all the answers

Which solution is used to wash out excess chromates from tissues fixed in Kelly’s, Zenker’s, and Flemming solutions?

Tap water Signup and view all the answers

What factor enhances the process of fixation?

Agitation Signup and view all the answers

How does cold temperature affect fixation?

Inactivates enzymes Signup and view all the answers

Which type of fixative is known as a microanatomical fixative?

Heidenhain’s Susa Formol Sublimate Signup and view all the answers

Why is washing out tap water used after fixing tissues?

To remove excess formalin and osmic acid Signup and view all the answers