Podcast
Questions and Answers
What is the recommended duration of formalin fixation?
What is the recommended duration of formalin fixation?
- 2 to 6 hours (correct)
- 4 to 8 hours
- 1 to 3 hours
- 6 to 10 hours
What is the ideal volume of fixative in relation to tissue volume for maximum effectiveness?
What is the ideal volume of fixative in relation to tissue volume for maximum effectiveness?
- 2 times the tissue volume
- 20 times the tissue volume (correct)
- Equal to tissue volume
- Less than tissue volume
What are the characteristics of a good fixative based on the text?
What are the characteristics of a good fixative based on the text?
- Cheap, stable, safe to handle (correct)
- Slow penetration, isotonic, inhibits staining procedures
- Expensive, unstable, unsafe
- Kills cells slowly, causes maximum shrinkage, enhances bacterial decomposition
What effect do fixatives have on tissues based on the text?
What effect do fixatives have on tissues based on the text?
What should be done if autopsy materials cannot be fixed immediately?
What should be done if autopsy materials cannot be fixed immediately?
What happens if fixation is prolonged according to the text?
What happens if fixation is prolonged according to the text?
What is the main goal of fixation in histotechnology?
What is the main goal of fixation in histotechnology?
Which type of fixation involves the fixing agent becoming part of the tissue by forming cross links?
Which type of fixation involves the fixing agent becoming part of the tissue by forming cross links?
What is the ideal pH range for fixation in histotechnology?
What is the ideal pH range for fixation in histotechnology?
In what temperature range are tissues processed for electron microscopy fixation?
In what temperature range are tissues processed for electron microscopy fixation?
What effect does hypertonic fixative have on cells during fixation?
What effect does hypertonic fixative have on cells during fixation?
What is the recommended concentration of glutaraldehyde for immuno electron microscopy fixation?
What is the recommended concentration of glutaraldehyde for immuno electron microscopy fixation?
What is the purpose of placing a primarily fixed tissue in a 2.5 - 3% potassium dichromate solution for 24 hours?
What is the purpose of placing a primarily fixed tissue in a 2.5 - 3% potassium dichromate solution for 24 hours?
Which solution is used to wash out excess chromates from tissues fixed in Kelly’s, Zenker’s, and Flemming solutions?
Which solution is used to wash out excess chromates from tissues fixed in Kelly’s, Zenker’s, and Flemming solutions?
What factor enhances the process of fixation?
What factor enhances the process of fixation?
How does cold temperature affect fixation?
How does cold temperature affect fixation?
Which type of fixative is known as a microanatomical fixative?
Which type of fixative is known as a microanatomical fixative?
Why is washing out tap water used after fixing tissues?
Why is washing out tap water used after fixing tissues?
