Histology and Tissue Preparation

What are the two main components of tissues?

Which macromolecules are primarily found in the extracellular matrix (ECM)?

What is the purpose of cutting thin sections of tissues for microscopy?

How do cells influence the extracellular matrix (ECM)?

What is the equivalent of one micrometer in millimeters?

What is the purpose of eosin in microscopy?

What is necessary for a proper understanding of tissue biology?

Which of the following methods utilizes the hexose rings of polysaccharides for staining?

During what stage do cells and matrix become functionally specialized?

What does the Feulgen reaction specifically stain?

What do tissue slices prepared for microscopic examination preserve?

What is the main challenge in examining most tissues and organs under light microscopy?

Which of the following processes is typically used to analyze biopsies during surgery?

What is the function of enzyme digestion in the identification process of basophilic material?

What can be stained distinctly purple using the PAS reaction?

Which spatial unit is equivalent to one angstrom?

What is the primary focus of histology?

Which microscopy technique is most suitable for viewing live cells?

What is the primary purpose of embedding fixed tissues?

What is an advantage of using electron microscopy?

Which of the following embedding materials is commonly used for light microscopy?

In which technique are specific molecules visualized through the use of antibodies?

What is the first step in preparing fixed tissue for embedding?

What is the purpose of tissue embedding in histology?

How do dyes behave when staining tissues?

What characteristic is unique to confocal microscopy compared to other light microscopy techniques?

What is usually the final ethanol concentration used during the dehydration process of fixed tissues?

Which microscopy method uses a beam of electrons to create images of fine details?

What is the role of staining in microscopy?

What is the main function of staining in histology?

What is a consequence of using plastic resins for embedding fixed tissues?

Which property of dyes is important for their function in staining tissues?

What staining method highlights cell surface glycoproteins and mucin in the epithelium lining the small intestine?

What color do the cell nuclei appear when stained with H&E?

Which component of the small intestine epithelium primarily secretes mucus?

How long might it take to prepare a slide from tissue fixation to microscopic observation?

What is the primary purpose of mounting a protective glass coverslip on a slide?

In what way are PAS-positive regions characterized in the epithelium?

What magnification factor contributes to the total magnification in microscopy?

How does the PAS reaction affect the staining intensity at the lumen of the small intestine epithelium?

Which of the following methods is used to preserve cell structures and make the tissue ready for sectioning in a biopsy?

What is the primary reason why frozen sections are preferred for studying lipids?

What is the primary function of the cryostat in the process described?

Which of the following is NOT a major benefit of using frozen tissue sections for histochemical studies?

Why is pretreatment with ribonuclease useful in examining cellular structures?

Which of the following staining techniques is used to visualize lipid-rich structures in cells?

What is the primary advantage of using frozen sections for the study of sensitive enzymes?

Which of the following is a common technique used to visualize certain ECM fibers and cellular elements in nervous tissue?