32 Questions
What is the primary focus of histology?
Study of the microscopic structure of tissues
What is the purpose of fixation in microtechnique?
To preserve objects for examination under the microscope
What is the final step in the paraffin technique for routine histological section?
Mounting
What is the purpose of deparaffinization in microtechnique?
To remove excess fixative
Why should tissue samples be obtained freshly from living tissue or very soon after death?
To prevent post-mortem changes in tissue
What is the purpose of washing in microtechnique?
To remove substances in the fixative which might interfere with subsequent processing
What is the purpose of dehydration in tissue preparation for sectioning?
To prevent distortion and damage of the tissue
Which substance is used for clearing in the paraffin method of tissue preparation?
Xylol
What is the purpose of microtomy and sectioning in tissue preparation?
To cut thin sections of tissue
What is the purpose of mounting in tissue preparation for sectioning?
To cover the section with a thin slip
What is the purpose of deparaffinization in tissue preparation?
To remove paraffin from the tissue
What is the purpose of hydration in tissue preparation for sectioning?
To prevent distortion and damage of the tissue
What is the purpose of staining in tissue preparation for sectioning?
To color specific components of the tissue
What are advantages of using paraffin in tissue preparation for sectioning?
It gives serial sections (ribbon)
What is a disadvantage of using paraffin in tissue preparation for sectioning?
The fixatives and heat used may damage tissues
What is a key difference between the celloidin method and the paraffin method of tissue preparation?
Celloidin method uses xylene for clearing
Which step in the paraffin technique for routine histological section involves removing excess fixative?
Washing
What is the purpose of fixation in microtechnique?
To preserve objects for examination under the microscope
What substance is used for clearing in the paraffin method of tissue preparation?
Xylene
Which substance is used for clearing in the paraffin method of tissue preparation?
Xylene
What is the purpose of deparaffinization in tissue preparation?
To remove paraffin from the tissue sections
What is the purpose of clearing in tissue preparation for sectioning?
To make the stained tissue sections transparent
Why should tissue samples be obtained freshly from living tissue or very soon after death?
To prevent distortion of tissue during cutting
Which method uses celloidin as an embedding material instead of paraffin?
Celloidin method
What is the primary focus of dehydration in tissue preparation for sectioning?
To remove water from the tissue
What is a key difference between the paraffin method and the celloidin method of tissue preparation?
Embedding material used
What is the primary focus of microtechnique?
Infiltration and Embedding
What is the purpose of rehydration in tissue preparation for sectioning?
To replace alcohol with distilled water
What is the purpose of microtomy and sectioning in tissue preparation?
To cut thin sections of tissue for examination
What are advantages of using paraffin in tissue preparation for sectioning?
It gives serial sections (ribbon)
What is a disadvantage of using paraffin in tissue preparation for sectioning?
The fixatives and heat used may damage the tissues.
What is the purpose of staining in tissue preparation for sectioning?
To highlight specific components within the tissue for visualization.
Test your knowledge on the microscopic anatomy of tissues and the art of preparing objects for examination under the microscope. This quiz covers topics such as tissue selection, fixation, dehydration, and microscopy techniques.
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