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Questions and Answers
What does Acid Fuchsin stain in Mallory's trichrome collagen stain?
What does Acridine Orange permit discrimination between?
What does Alcian Blue stain specifically?
What color does Alizarin Red S form with calcium at a pH of 4.2?
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What is Aniline Blue used for?
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Which stain is used as a contrast stain for Gram's technique and in the Papanicolau method?
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What is BASIC FUCHSIN primarily used for?
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What is the main constituent of Feulgen's and Schiff's reagent for the detection of aldehyde?
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What is the primary stain used in acid-fast staining?
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What is Celestine Blue primarily used for?
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Study Notes
Staining Techniques and Applications
- Acid Fuchsin: Stains collagen in Mallory's trichrome stain, highlighting connective tissue structures.
- Acridine Orange: Allows differentiation between living (green fluorescence) and dead (orange/red fluorescence) cells, useful in cytology.
- Alcian Blue: Specifically stains acidic mucins and glycosaminoglycans, aiding in the identification of certain types of tissues.
- Alizarin Red S: Forms a red complex with calcium at a pH of 4.2, used to identify calcium deposits in tissues.
- Aniline Blue: Employed to visualize collagen fibers, particularly in histological studies related to connective tissues.
- Contrast Stain for Gram's Technique: Crystal violet serves as a primary stain, while safranin acts as the counter stain in the Papanicolaou method for cytology.
- BASIC FUCHSIN: Primarily used to stain elastic fibers, facilitating the study of vascular structures and tissue integrity.
- Feulgen's and Schiff's Reagent: The main constituent is the aldehyde compound, which specifically binds to DNA, important for nuclear staining.
- Primary Stain in Acid-Fast Staining: Carbol fuchsin is the primary stain, especially crucial for identifying Mycobacteria.
- Celestine Blue: Mainly used for the staining of ribosomal RNA in RNA-rich cell types, enhancing the visibility of these structures in histological preparations.
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Description
Test your knowledge of histological staining techniques and dyes with this quiz. Explore the properties and applications of acid fuchsin, Mallory's trichrome stain, and Van Gieson's stain, and learn about other related stains like acridine orange.
