Histological Staining Techniques and Dyes Quiz

Staining Techniques and Applications

• Acid Fuchsin: Stains collagen in Mallory's trichrome stain, highlighting connective tissue structures.
• Acridine Orange: Allows differentiation between living (green fluorescence) and dead (orange/red fluorescence) cells, useful in cytology.
• Alcian Blue: Specifically stains acidic mucins and glycosaminoglycans, aiding in the identification of certain types of tissues.
• Alizarin Red S: Forms a red complex with calcium at a pH of 4.2, used to identify calcium deposits in tissues.
• Aniline Blue: Employed to visualize collagen fibers, particularly in histological studies related to connective tissues.
• Contrast Stain for Gram's Technique: Crystal violet serves as a primary stain, while safranin acts as the counter stain in the Papanicolaou method for cytology.
• BASIC FUCHSIN: Primarily used to stain elastic fibers, facilitating the study of vascular structures and tissue integrity.
• Feulgen's and Schiff's Reagent: The main constituent is the aldehyde compound, which specifically binds to DNA, important for nuclear staining.
• Primary Stain in Acid-Fast Staining: Carbol fuchsin is the primary stain, especially crucial for identifying Mycobacteria.
• Celestine Blue: Mainly used for the staining of ribosomal RNA in RNA-rich cell types, enhancing the visibility of these structures in histological preparations.