Acid-Fast Staining Methodology

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Who is the bacteriologist credited with devising the Gram staining method?

Hans Christian Andersen

Louis Pasteur

Robert Koch

Hans Christian Gram (correct)

What is the primary stain used in the Gram's staining method?

Iodine

Safranin

Methylene Blue

Crystal Violet (correct)

Which color do microorganisms appear if they retain the crystal violet-iodine complex in the Gram staining method?

Green

Blue

Purple brown (correct)

Red

What are microorganisms classified as if they are stained by the Gram's method?

Gram-positive or Gram non-negative Signup and view all the answers

What happens to microorganisms that are not stained by crystal violet in the Gram staining method?

They appear red Signup and view all the answers

What is the purpose of adding iodine solution on the smear during the Gram staining procedure?

To cover the fixed culture Signup and view all the answers

Why are non-acid fast organisms easily decolorized with acid alcohol during AFB staining?

Because they take up the counterstain readily Signup and view all the answers

What is the preferred specimen for AFB staining according to the text?

Early morning sputum Signup and view all the answers

What should a patient avoid doing prior to providing a sputum sample?

Gargling with mouthwash Signup and view all the answers

Why does the text recommend not opening AFB samples in the open?

To prevent contamination of the sample Signup and view all the answers

How should spot-AM sputum samples be collected according to the text?

First specimen at first consultation, second specimen in the morning Signup and view all the answers

Why is aerosol-induced collection recommended for ambulatory patients?

To improve sample quality by inducing coughing Signup and view all the answers

What is the next step after preparing the specimen smear for AFB staining?

Flood the slide with aqueous carbol fuchsin solution. Signup and view all the answers

What is the purpose of placing a strip of blotting paper or filter paper onto the slide during AFB staining?

To prevent the preparation from drying up. Signup and view all the answers

What should be done after allowing the sputum smear to air dry for 15 minutes?

Fix by flaming while moving the slide in a circular direction. Signup and view all the answers

What is the correct way to dispose of the disposable inoculating loop/applicator stick used in AFB staining?

Submerge in a container with sand and Sodium hypochlorite solution before disposal. Signup and view all the answers

What should be avoided during the fixing step of sputum smear preparation?

Overheating the slide. Signup and view all the answers

What is the purpose of washing off the excess stain with tap water during AFB staining?

To remove excess stain after staining. Signup and view all the answers