Podcast
Questions and Answers
Who is the bacteriologist credited with devising the Gram staining method?
Who is the bacteriologist credited with devising the Gram staining method?
- Hans Christian Andersen
- Louis Pasteur
- Robert Koch
- Hans Christian Gram (correct)
What is the primary stain used in the Gram's staining method?
What is the primary stain used in the Gram's staining method?
- Iodine
- Safranin
- Methylene Blue
- Crystal Violet (correct)
Which color do microorganisms appear if they retain the crystal violet-iodine complex in the Gram staining method?
Which color do microorganisms appear if they retain the crystal violet-iodine complex in the Gram staining method?
- Green
- Blue
- Purple brown (correct)
- Red
What are microorganisms classified as if they are stained by the Gram's method?
What are microorganisms classified as if they are stained by the Gram's method?
What happens to microorganisms that are not stained by crystal violet in the Gram staining method?
What happens to microorganisms that are not stained by crystal violet in the Gram staining method?
What is the purpose of adding iodine solution on the smear during the Gram staining procedure?
What is the purpose of adding iodine solution on the smear during the Gram staining procedure?
Why are non-acid fast organisms easily decolorized with acid alcohol during AFB staining?
Why are non-acid fast organisms easily decolorized with acid alcohol during AFB staining?
What is the preferred specimen for AFB staining according to the text?
What is the preferred specimen for AFB staining according to the text?
What should a patient avoid doing prior to providing a sputum sample?
What should a patient avoid doing prior to providing a sputum sample?
Why does the text recommend not opening AFB samples in the open?
Why does the text recommend not opening AFB samples in the open?
How should spot-AM sputum samples be collected according to the text?
How should spot-AM sputum samples be collected according to the text?
Why is aerosol-induced collection recommended for ambulatory patients?
Why is aerosol-induced collection recommended for ambulatory patients?
What is the next step after preparing the specimen smear for AFB staining?
What is the next step after preparing the specimen smear for AFB staining?
What is the purpose of placing a strip of blotting paper or filter paper onto the slide during AFB staining?
What is the purpose of placing a strip of blotting paper or filter paper onto the slide during AFB staining?
What should be done after allowing the sputum smear to air dry for 15 minutes?
What should be done after allowing the sputum smear to air dry for 15 minutes?
What is the correct way to dispose of the disposable inoculating loop/applicator stick used in AFB staining?
What is the correct way to dispose of the disposable inoculating loop/applicator stick used in AFB staining?
What should be avoided during the fixing step of sputum smear preparation?
What should be avoided during the fixing step of sputum smear preparation?
What is the purpose of washing off the excess stain with tap water during AFB staining?
What is the purpose of washing off the excess stain with tap water during AFB staining?
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