Histological Staining and Tissue Processing Procedure Quiz
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Questions and Answers

What is the purpose of the eyepiece in a microscope?

  • Rotates to hold objective lenses
  • Contains lenses for magnification (correct)
  • Moves the body tube or stage up and down to focus the image
  • Supports the body tube and lenses

What is the function of the revolving nosepiece (turret) in a microscope?

  • Contain objective lenses
  • Sharpening the focusing image
  • Rotating device to which objective lenses are attached (correct)
  • Supporting the slide being viewed

How does the coarse adjustment knob in a microscope help with focusing?

  • Contains lenses for magnification
  • Sharpening the focusing image
  • Rotates to hold objective lenses
  • Moves the body tube or stage up and down to focus the image (correct)

What is the first step in tissue processing?

<p>Fixation (B)</p> Signup and view all the answers

Which part of a microscope maintains a set distance between the eyepiece and the objective lenses?

<p>Body tube (barrel) (B)</p> Signup and view all the answers

Which staining method is used to stain components with Periodic Schiff reaction?

<p>Haematoxylin and Eosin staining (B)</p> Signup and view all the answers

What is the main function of the stage in a microscope?

<p>Supports the slide being viewed (D)</p> Signup and view all the answers

In which step of tissue processing are sections cut and mounted?

<p>Fixation (C)</p> Signup and view all the answers

What type of nucleus does an actively synthesizing cell usually have?

<p>Large, pale staining nucleus (D)</p> Signup and view all the answers

What does pink-staining granular cytoplasm often indicate?

<p>Presence of cytoplasmic RNA in ribosomes (A)</p> Signup and view all the answers

What is the purpose of the arm in a microscope?

<p>Supports the body tube and lenses (C)</p> Signup and view all the answers

What does diffuse purple-tinted cytoplasm indicate?

<p>Presence of cytoplasmic RNA in ribosomes (C)</p> Signup and view all the answers

What is the relationship between milli-, micro-, and nanometers?

<p>Micrometers are smaller than millimeters but larger than nanometers. (C)</p> Signup and view all the answers

Why is it important to fix tissues?

<p>To prevent the tissues from decomposing. (D)</p> Signup and view all the answers

Which biopsy technique involves the use of a needle?

<p>Fine-needle aspiration biopsy (A)</p> Signup and view all the answers

What are shrinkage artifacts in tissue processing?

<p>Artificial changes in tissue size due to inadequate fixation. (C)</p> Signup and view all the answers

Which staining technique involves the components Periodic acid Schiff (PAS) and Haematoxylin and Eosin (H & E)?

<p>Trichrome staining (B)</p> Signup and view all the answers

Which type of microscopy offers advantages in visualizing live cells and dynamic processes?

<p>Fluorescence microscopy (C)</p> Signup and view all the answers

What is the main purpose of a biopsy?

<p>To analyze a small piece of tissue under a microscope (B)</p> Signup and view all the answers

Which tissue type is responsible for supporting other tissues in the body?

<p>Connective tissue (C)</p> Signup and view all the answers

What is the average diameter of most human cells?

<p>$10$ µm (D)</p> Signup and view all the answers

Which biopsy technique involves the removal of tissue through a small surgical instrument?

<p>Curettage biopsy (B)</p> Signup and view all the answers

What is the relationship between millimeters, micrometers, and nanometers?

<p>$1$ mm = $1000$ µm = $1,000,000$ nm (B)</p> Signup and view all the answers

Why is it important to fix tissues before examination?

<p>To preserve cellular structures and prevent decay (D)</p> Signup and view all the answers

What is the formula for calculating resolution in a microscope?

<p>R = 0.61 λ / NA (D)</p> Signup and view all the answers

What is the main advantage of electron microscopes over light microscopes?

<p>Better resolution (A)</p> Signup and view all the answers

In a Scanning Electron Microscope (SEM), how are images produced?

<p>By scanning the specimen with a focused electron beam (C)</p> Signup and view all the answers

Which type of microscope produces Stereoscopic images for specimens?

<p>Scanning Electron Microscope (SEM) (A)</p> Signup and view all the answers

During the preparation of a routine H&E slide, what process allows the tissue to be visualized?

<p>Staining (D)</p> Signup and view all the answers

What is the bottom part of a microscope called?

<p>Base (C)</p> Signup and view all the answers

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