30 Questions
What is the purpose of the eyepiece in a microscope?
Contains lenses for magnification
What is the function of the revolving nosepiece (turret) in a microscope?
Rotating device to which objective lenses are attached
How does the coarse adjustment knob in a microscope help with focusing?
Moves the body tube or stage up and down to focus the image
What is the first step in tissue processing?
Fixation
Which part of a microscope maintains a set distance between the eyepiece and the objective lenses?
Body tube (barrel)
Which staining method is used to stain components with Periodic Schiff reaction?
Haematoxylin and Eosin staining
What is the main function of the stage in a microscope?
Supports the slide being viewed
In which step of tissue processing are sections cut and mounted?
Fixation
What type of nucleus does an actively synthesizing cell usually have?
Large, pale staining nucleus
What does pink-staining granular cytoplasm often indicate?
Presence of cytoplasmic RNA in ribosomes
What is the purpose of the arm in a microscope?
Supports the body tube and lenses
What does diffuse purple-tinted cytoplasm indicate?
Presence of cytoplasmic RNA in ribosomes
What is the relationship between milli-, micro-, and nanometers?
Micrometers are smaller than millimeters but larger than nanometers.
Why is it important to fix tissues?
To prevent the tissues from decomposing.
Which biopsy technique involves the use of a needle?
Fine-needle aspiration biopsy
What are shrinkage artifacts in tissue processing?
Artificial changes in tissue size due to inadequate fixation.
Which staining technique involves the components Periodic acid Schiff (PAS) and Haematoxylin and Eosin (H & E)?
Trichrome staining
Which type of microscopy offers advantages in visualizing live cells and dynamic processes?
Fluorescence microscopy
What is the main purpose of a biopsy?
To analyze a small piece of tissue under a microscope
Which tissue type is responsible for supporting other tissues in the body?
Connective tissue
What is the average diameter of most human cells?
$10$ µm
Which biopsy technique involves the removal of tissue through a small surgical instrument?
Curettage biopsy
What is the relationship between millimeters, micrometers, and nanometers?
$1$ mm = $1000$ µm = $1,000,000$ nm
Why is it important to fix tissues before examination?
To preserve cellular structures and prevent decay
What is the formula for calculating resolution in a microscope?
R = 0.61 λ / NA
What is the main advantage of electron microscopes over light microscopes?
Better resolution
In a Scanning Electron Microscope (SEM), how are images produced?
By scanning the specimen with a focused electron beam
Which type of microscope produces Stereoscopic images for specimens?
Scanning Electron Microscope (SEM)
During the preparation of a routine H&E slide, what process allows the tissue to be visualized?
Staining
What is the bottom part of a microscope called?
Base
Test your knowledge on tissue processing procedures including fixation, dehydration, wax embedding, cutting, staining, and mounting. Explore the value of histological staining and identify the components stained by Periodic Schiff reaction and Haematoxylin and Eosin staining. Learn about the characteristics of the nucleus in actively synthesizing cells.
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