Histological Staining and Tissue Processing Procedure Quiz

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What is the purpose of the eyepiece in a microscope?

Rotates to hold objective lenses

Contains lenses for magnification (correct)

Moves the body tube or stage up and down to focus the image

Supports the body tube and lenses

What is the function of the revolving nosepiece (turret) in a microscope?

Contain objective lenses

Sharpening the focusing image

Rotating device to which objective lenses are attached (correct)

Supporting the slide being viewed

How does the coarse adjustment knob in a microscope help with focusing?

Contains lenses for magnification

Sharpening the focusing image

Rotates to hold objective lenses

Moves the body tube or stage up and down to focus the image (correct)

What is the first step in tissue processing?

Fixation Signup and view all the answers

Which part of a microscope maintains a set distance between the eyepiece and the objective lenses?

Body tube (barrel) Signup and view all the answers

Which staining method is used to stain components with Periodic Schiff reaction?

Haematoxylin and Eosin staining Signup and view all the answers

What is the main function of the stage in a microscope?

Supports the slide being viewed Signup and view all the answers

In which step of tissue processing are sections cut and mounted?

Fixation Signup and view all the answers

What type of nucleus does an actively synthesizing cell usually have?

Large, pale staining nucleus Signup and view all the answers

What does pink-staining granular cytoplasm often indicate?

Presence of cytoplasmic RNA in ribosomes Signup and view all the answers

What is the purpose of the arm in a microscope?

Supports the body tube and lenses Signup and view all the answers

What does diffuse purple-tinted cytoplasm indicate?

Presence of cytoplasmic RNA in ribosomes Signup and view all the answers

What is the relationship between milli-, micro-, and nanometers?

Micrometers are smaller than millimeters but larger than nanometers. Signup and view all the answers

Why is it important to fix tissues?

To prevent the tissues from decomposing. Signup and view all the answers

Which biopsy technique involves the use of a needle?

Fine-needle aspiration biopsy Signup and view all the answers

What are shrinkage artifacts in tissue processing?

Artificial changes in tissue size due to inadequate fixation. Signup and view all the answers

Which staining technique involves the components Periodic acid Schiff (PAS) and Haematoxylin and Eosin (H & E)?

Trichrome staining Signup and view all the answers

Which type of microscopy offers advantages in visualizing live cells and dynamic processes?

Fluorescence microscopy Signup and view all the answers

What is the main purpose of a biopsy?

To analyze a small piece of tissue under a microscope Signup and view all the answers

Which tissue type is responsible for supporting other tissues in the body?

Connective tissue Signup and view all the answers

What is the average diameter of most human cells?

$10$ µm Signup and view all the answers

Which biopsy technique involves the removal of tissue through a small surgical instrument?

Curettage biopsy Signup and view all the answers

What is the relationship between millimeters, micrometers, and nanometers?

$1$ mm = $1000$ µm = $1,000,000$ nm Signup and view all the answers

Why is it important to fix tissues before examination?

To preserve cellular structures and prevent decay Signup and view all the answers

What is the formula for calculating resolution in a microscope?

R = 0.61 λ / NA Signup and view all the answers

What is the main advantage of electron microscopes over light microscopes?

Better resolution Signup and view all the answers

In a Scanning Electron Microscope (SEM), how are images produced?

By scanning the specimen with a focused electron beam Signup and view all the answers

Which type of microscope produces Stereoscopic images for specimens?

Scanning Electron Microscope (SEM) Signup and view all the answers

During the preparation of a routine H&E slide, what process allows the tissue to be visualized?

Staining Signup and view all the answers

What is the bottom part of a microscope called?

Base Signup and view all the answers