Histological Staining and Tissue Processing Procedure Quiz
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Histological Staining and Tissue Processing Procedure Quiz

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Questions and Answers

What is the purpose of the eyepiece in a microscope?

  • Rotates to hold objective lenses
  • Contains lenses for magnification (correct)
  • Moves the body tube or stage up and down to focus the image
  • Supports the body tube and lenses

    • What is the function of the revolving nosepiece (turret) in a microscope?

  • Contain objective lenses
  • Sharpening the focusing image
  • Rotating device to which objective lenses are attached (correct)
  • Supporting the slide being viewed

    • How does the coarse adjustment knob in a microscope help with focusing?

  • Contains lenses for magnification
  • Sharpening the focusing image
  • Rotates to hold objective lenses
  • Moves the body tube or stage up and down to focus the image (correct)

    • What is the first step in tissue processing?

    <p>Fixation</p> Signup and view all the answers

    Which part of a microscope maintains a set distance between the eyepiece and the objective lenses?

    <p>Body tube (barrel)</p> Signup and view all the answers

    Which staining method is used to stain components with Periodic Schiff reaction?

    <p>Haematoxylin and Eosin staining</p> Signup and view all the answers

    What is the main function of the stage in a microscope?

    <p>Supports the slide being viewed</p> Signup and view all the answers

    In which step of tissue processing are sections cut and mounted?

    <p>Fixation</p> Signup and view all the answers

    What type of nucleus does an actively synthesizing cell usually have?

    <p>Large, pale staining nucleus</p> Signup and view all the answers

    What does pink-staining granular cytoplasm often indicate?

    <p>Presence of cytoplasmic RNA in ribosomes</p> Signup and view all the answers

    What is the purpose of the arm in a microscope?

    <p>Supports the body tube and lenses</p> Signup and view all the answers

    What does diffuse purple-tinted cytoplasm indicate?

    <p>Presence of cytoplasmic RNA in ribosomes</p> Signup and view all the answers

    What is the relationship between milli-, micro-, and nanometers?

    <p>Micrometers are smaller than millimeters but larger than nanometers.</p> Signup and view all the answers

    Why is it important to fix tissues?

    <p>To prevent the tissues from decomposing.</p> Signup and view all the answers

    Which biopsy technique involves the use of a needle?

    <p>Fine-needle aspiration biopsy</p> Signup and view all the answers

    What are shrinkage artifacts in tissue processing?

    <p>Artificial changes in tissue size due to inadequate fixation.</p> Signup and view all the answers

    Which staining technique involves the components Periodic acid Schiff (PAS) and Haematoxylin and Eosin (H & E)?

    <p>Trichrome staining</p> Signup and view all the answers

    Which type of microscopy offers advantages in visualizing live cells and dynamic processes?

    <p>Fluorescence microscopy</p> Signup and view all the answers

    What is the main purpose of a biopsy?

    <p>To analyze a small piece of tissue under a microscope</p> Signup and view all the answers

    Which tissue type is responsible for supporting other tissues in the body?

    <p>Connective tissue</p> Signup and view all the answers

    What is the average diameter of most human cells?

    <p>$10$ µm</p> Signup and view all the answers

    Which biopsy technique involves the removal of tissue through a small surgical instrument?

    <p>Curettage biopsy</p> Signup and view all the answers

    What is the relationship between millimeters, micrometers, and nanometers?

    <p>$1$ mm = $1000$ µm = $1,000,000$ nm</p> Signup and view all the answers

    Why is it important to fix tissues before examination?

    <p>To preserve cellular structures and prevent decay</p> Signup and view all the answers

    What is the formula for calculating resolution in a microscope?

    <p>R = 0.61 λ / NA</p> Signup and view all the answers

    What is the main advantage of electron microscopes over light microscopes?

    <p>Better resolution</p> Signup and view all the answers

    In a Scanning Electron Microscope (SEM), how are images produced?

    <p>By scanning the specimen with a focused electron beam</p> Signup and view all the answers

    Which type of microscope produces Stereoscopic images for specimens?

    <p>Scanning Electron Microscope (SEM)</p> Signup and view all the answers

    During the preparation of a routine H&E slide, what process allows the tissue to be visualized?

    <p>Staining</p> Signup and view all the answers

    What is the bottom part of a microscope called?

    <p>Base</p> Signup and view all the answers

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