HPLC Quiz: Master High-Performance Liquid Chromatography

Questions and Answers

To separate, identify, and quantify specific components in mixtures

Interact with sample components to cause separation

Deliver mixtures of various solvents as the mobile phase

Graphical representations of signal intensity versus time or volume Signup and view all the answers

Manufacturing, legal, and research sectors Signup and view all the answers

Topical antiseptic Signup and view all the answers

Isopropyl alcohol or ethyl alcohol products Signup and view all the answers

70 to 99% v/v Signup and view all the answers

Surgical spirit B.P. Signup and view all the answers

Bitterants added Signup and view all the answers

Study Notes

HPLC is a technique used to separate, identify, and quantify the components of a mixture.
The purpose of HPLC is to analyze the composition of complex samples, such as pharmaceuticals, food, and environmental samples.

The solid particles in the column of HPLC are used to separate the components of the mixture based on their interactions with the particles.
The particles are typically porous and have a high surface area, allowing for effective separation of the mixture's components.

The high-pressure pumps in HPLC are used to force the mobile phase through the column at high pressures (up to 400 bar).
The high pressure enables the efficient separation of the mixture's components within a reasonable time frame.

Chromatograms are graphical representations of the separation of the mixture's components.
They represent the detector response to the individual components as they elute from the column.

HPLC is widely used in the pharmaceutical, biotechnological, and food industries, as well as in environmental monitoring and scientific research.

Rubbing alcohol, also known as isopropyl alcohol, is primarily used as a topical antiseptic, a disinfectant, and a solvent.
In North American English, the term "rubbing alcohol" refers to a specific preparation of isopropyl alcohol, typically containing 70% or 91% isopropyl alcohol.
The ethanol content range for retail bottles of rubbing alcohol varies, but is typically between 70% and 99%.
The British Pharmacopoeia (BP) refers to the comparable preparation to rubbing alcohol as "isopropyl alcohol (rubbing)".
Rubbing alcohol is made undrinkable by adding a bittering agent, such as denatonium benzoate, to prevent accidental ingestion.