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Questions and Answers
What is the purpose of HPLC in analytical chemistry?
What is the purpose of HPLC in analytical chemistry?
What is the purpose of the solid particles in the column of HPLC?
What is the purpose of the solid particles in the column of HPLC?
What is the function of the high pressure pumps in HPLC?
What is the function of the high pressure pumps in HPLC?
What do chromatograms represent?
What do chromatograms represent?
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Where is HPLC widely used?
Where is HPLC widely used?
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What is rubbing alcohol primarily used for?
What is rubbing alcohol primarily used for?
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What does the term 'rubbing alcohol' refer to in North American English?
What does the term 'rubbing alcohol' refer to in North American English?
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What is the ethanol content range for retail bottles of rubbing alcohol?
What is the ethanol content range for retail bottles of rubbing alcohol?
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What does the British Pharmacopoeia (BP) refer to as the comparable preparation to rubbing alcohol?
What does the British Pharmacopoeia (BP) refer to as the comparable preparation to rubbing alcohol?
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What makes rubbing alcohol undrinkable even if it is ethanol-based?
What makes rubbing alcohol undrinkable even if it is ethanol-based?
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Study Notes
HPLC (High-Performance Liquid Chromatography) in Analytical Chemistry
- HPLC is a technique used to separate, identify, and quantify the components of a mixture.
- The purpose of HPLC is to analyze the composition of complex samples, such as pharmaceuticals, food, and environmental samples.
HPLC Column and Solid Particles
- The solid particles in the column of HPLC are used to separate the components of the mixture based on their interactions with the particles.
- The particles are typically porous and have a high surface area, allowing for effective separation of the mixture's components.
HPLC Pumps
- The high-pressure pumps in HPLC are used to force the mobile phase through the column at high pressures (up to 400 bar).
- The high pressure enables the efficient separation of the mixture's components within a reasonable time frame.
Chromatograms
- Chromatograms are graphical representations of the separation of the mixture's components.
- They represent the detector response to the individual components as they elute from the column.
Applications of HPLC
- HPLC is widely used in the pharmaceutical, biotechnological, and food industries, as well as in environmental monitoring and scientific research.
Rubbing Alcohol
- Rubbing alcohol, also known as isopropyl alcohol, is primarily used as a topical antiseptic, a disinfectant, and a solvent.
- In North American English, the term "rubbing alcohol" refers to a specific preparation of isopropyl alcohol, typically containing 70% or 91% isopropyl alcohol.
- The ethanol content range for retail bottles of rubbing alcohol varies, but is typically between 70% and 99%.
- The British Pharmacopoeia (BP) refers to the comparable preparation to rubbing alcohol as "isopropyl alcohol (rubbing)".
- Rubbing alcohol is made undrinkable by adding a bittering agent, such as denatonium benzoate, to prevent accidental ingestion.
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Description
Test your knowledge of high-performance liquid chromatography (HPLC), a technique used in analytical chemistry to separate, identify, and quantify specific components in various mixtures. This quiz covers the principles, applications, and equipment used in HPLC.
