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Questions and Answers
What is the main purpose of using high pressure in HPLC?
What is the main purpose of using high pressure in HPLC?
- To increase the flow rate of the mobile phase
- To improve the separation efficiency of the stationary phase
- To withstand the force of the pumped solvent through the column (correct)
- To decrease the particle size of the stationary phase
What is the typical range of flow rates used in modern HPLC systems?
What is the typical range of flow rates used in modern HPLC systems?
- 5 - 10 ml/min
- 1 - 3 ml/min (correct)
- 0.1 - 1 ml/min
- 10 - 20 ml/min
What is the main advantage of using longer HPLC columns?
What is the main advantage of using longer HPLC columns?
- Lower operating pressures
- Reduced solvent consumption
- Improved separation efficiency (correct)
- Faster analysis times
Which of the following is NOT a common particle size used in modern HPLC columns?
Which of the following is NOT a common particle size used in modern HPLC columns?
What is the main purpose of the stationary phase in an HPLC system?
What is the main purpose of the stationary phase in an HPLC system?
Which of the following is a major advantage of using HPLC over other chromatographic techniques?
Which of the following is a major advantage of using HPLC over other chromatographic techniques?
What is the function of the sample injector in an HPLC instrument?
What is the function of the sample injector in an HPLC instrument?
What is a key identifying characteristic of a given analyte in HPLC?
What is a key identifying characteristic of a given analyte in HPLC?
In HPLC, which component moves through the column at different velocities depending on its chemical nature?
In HPLC, which component moves through the column at different velocities depending on its chemical nature?
What does the term 'retention time' refer to in the context of HPLC?
What does the term 'retention time' refer to in the context of HPLC?
Which component of an HPLC instrument is responsible for displaying data as a chromatogram?
Which component of an HPLC instrument is responsible for displaying data as a chromatogram?
What is the role of the stationary phase (column) in HPLC separation?
What is the role of the stationary phase (column) in HPLC separation?
In HILIC, what compounds are easier to separate compared to RPLC?
In HILIC, what compounds are easier to separate compared to RPLC?
What is the elution order difference between HILIC and RPLC?
What is the elution order difference between HILIC and RPLC?
Which type of chromatography technique fills a gap in the chromatographic toolbox according to the text?
Which type of chromatography technique fills a gap in the chromatographic toolbox according to the text?
What compounds are troublesome to separate in RPLC but are much easier to separate in HILIC?
What compounds are troublesome to separate in RPLC but are much easier to separate in HILIC?
What type of detectors are generally used in ion chromatography?
What type of detectors are generally used in ion chromatography?
What does the conductivity detector measure in ion chromatography?
What does the conductivity detector measure in ion chromatography?
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