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High Performance Liquid Chromatography (HPLC)

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High Performance Liquid Chromatography (HPLC)

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Questions and Answers

What is the main purpose of using high pressure in HPLC?

To increase the flow rate of the mobile phase

To improve the separation efficiency of the stationary phase

To withstand the force of the pumped solvent through the column (correct)

To decrease the particle size of the stationary phase

What is the typical range of flow rates used in modern HPLC systems?

5 - 10 ml/min

1 - 3 ml/min (correct)

0.1 - 1 ml/min

10 - 20 ml/min

What is the main advantage of using longer HPLC columns?

Lower operating pressures

Reduced solvent consumption

Improved separation efficiency (correct)

Faster analysis times

Which of the following is NOT a common particle size used in modern HPLC columns?

15 μm Signup and view all the answers

What is the main purpose of the stationary phase in an HPLC system?

To separate the components of the sample mixture Signup and view all the answers

Which of the following is a major advantage of using HPLC over other chromatographic techniques?

Higher separation efficiency Signup and view all the answers

What is the function of the sample injector in an HPLC instrument?

Introducing the sample mixture into the stream of mobile phase Signup and view all the answers

What is a key identifying characteristic of a given analyte in HPLC?

The retention time Signup and view all the answers

In HPLC, which component moves through the column at different velocities depending on its chemical nature?

Analyte components Signup and view all the answers

What does the term 'retention time' refer to in the context of HPLC?

Time at which a specific analyte elutes from the column Signup and view all the answers

Which component of an HPLC instrument is responsible for displaying data as a chromatogram?

Detector Signup and view all the answers

What is the role of the stationary phase (column) in HPLC separation?

Determining the retention time of analytes Signup and view all the answers

In HILIC, what compounds are easier to separate compared to RPLC?

Polar and hydrophilic compounds Signup and view all the answers

What is the elution order difference between HILIC and RPLC?

Reverse Signup and view all the answers

Which type of chromatography technique fills a gap in the chromatographic toolbox according to the text?

HILIC Signup and view all the answers

What compounds are troublesome to separate in RPLC but are much easier to separate in HILIC?

Acids, bases, ions, and sugars Signup and view all the answers

What type of detectors are generally used in ion chromatography?

Conductivity detectors Signup and view all the answers

What does the conductivity detector measure in ion chromatography?

Electrical resistance of the mobile phase Signup and view all the answers