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Questions and Answers
What software was used for image editing?
What software was used for image editing?
Which kit was used for extracting total cellular DNA?
Which kit was used for extracting total cellular DNA?
What is the purpose of the restriction digestion reaction?
What is the purpose of the restriction digestion reaction?
Which enzyme was used in the restriction digestion reaction?
Which enzyme was used in the restriction digestion reaction?
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What is the purpose of circularizing the digested fragments?
What is the purpose of circularizing the digested fragments?
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What is the purpose of adding T4 DNA Ligase in the reaction?
What is the purpose of adding T4 DNA Ligase in the reaction?
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What is represented by the dashed black lines in Fig. 3B?
What is represented by the dashed black lines in Fig. 3B?
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Which cell type was infected with a Gaussia luciferase-expressing reporter HBV?
Which cell type was infected with a Gaussia luciferase-expressing reporter HBV?
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What does cccDNA stand for in the given context?
What does cccDNA stand for in the given context?
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What do the light lines connecting data points in Fig. 2A represent?
What do the light lines connecting data points in Fig. 2A represent?
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In which setup were HepG2-NTCP cells serially split after infection?
In which setup were HepG2-NTCP cells serially split after infection?
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What does HBc refer to in the context of the experiment?
What does HBc refer to in the context of the experiment?
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What was found regarding the protein expression from cccDNA in relation to cccDNA copy number?
What was found regarding the protein expression from cccDNA in relation to cccDNA copy number?
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Which technique was found to be insufficiently sensitive to detect low levels of secretion from cccDNA?
Which technique was found to be insufficiently sensitive to detect low levels of secretion from cccDNA?
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What was the effect observed on the number of HBc-positive cells with each additional split?
What was the effect observed on the number of HBc-positive cells with each additional split?
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What does MyrB represent in the context of the study?
What does MyrB represent in the context of the study?
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What was the purpose of visualizing HBV-infected HepG2-NTCP cells using fluorescence microscopy?
What was the purpose of visualizing HBV-infected HepG2-NTCP cells using fluorescence microscopy?
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Which model is used to describe the expected number of HBc-positive cells following mitosis?
Which model is used to describe the expected number of HBc-positive cells following mitosis?
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What was the main difference between setup 1 (green) and setup 2 (purple) in the experimental setups?
What was the main difference between setup 1 (green) and setup 2 (purple) in the experimental setups?
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What do the top and bottom dashed lines in the dilution and loss models predict?
What do the top and bottom dashed lines in the dilution and loss models predict?
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What does the area between the dilution and loss models represent?
What does the area between the dilution and loss models represent?
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What do higher levels of cccDNA compared to the dilution model suggest?
What do higher levels of cccDNA compared to the dilution model suggest?
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What is represented by levels lower than the loss model in terms of cccDNA post-mitosis?
What is represented by levels lower than the loss model in terms of cccDNA post-mitosis?
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In what type of cells were the experiments conducted involving setup 1 and setup 2?
In what type of cells were the experiments conducted involving setup 1 and setup 2?
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What was observed about daughter cells of HBV-infected hepatocytes after mitosis?
What was observed about daughter cells of HBV-infected hepatocytes after mitosis?
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What was the fate of individual HBV-infected cells that undergo mitosis?
What was the fate of individual HBV-infected cells that undergo mitosis?
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What was used to track living HBV-infected cells in the study?
What was used to track living HBV-infected cells in the study?
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What was the concentration of HepG2-NTCP cells infected with tRFP-HBV seeded in chamber slides?
What was the concentration of HepG2-NTCP cells infected with tRFP-HBV seeded in chamber slides?
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What was observed in high-confluence areas when it came to tRFP expression in non-dividing cells?
What was observed in high-confluence areas when it came to tRFP expression in non-dividing cells?
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What was the outcome after photo-bleaching all existing tRFP and allowing the cells to recover?
What was the outcome after photo-bleaching all existing tRFP and allowing the cells to recover?
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