Questions and Answers
What is the preferred anticoagulant for mean platelet volume (MPV) according to Turgeon?
The size of platelets increases in the first hour after EDTA collection.
True
What is the proper ratio of EDTA to whole blood for optimal results?
1.5 mg/mL
What is the effect of excessive EDTA in blood samples?
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EDTA is found in the ____ top tube.
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What does EDTA do to calcium in the blood?
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How many inversions should the lavender/purple top tube undergo after filling?
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In healthy patients, the platelet count and size have a direct relationship.
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Study Notes
Anticoagulants
- EDTA (Ethylenediaminetetraacetic Acid) is the preferred anticoagulant in hematology for mean platelet volume (MPV) analysis.
- Prolonged exposure to EDTA can cause platelets to swell, increasing MPV by approximately 20% within the first hour; stability occurs after this duration for at least 12 hours.
- MPV measurements should utilize EDTA specimens aged between 1 and 4 hours for accuracy.
Use and Properties of EDTA
- EDTA is commonly found in lavender or purple top tubes.
- The optimal ratio of EDTA to whole blood is critical; incorrect ratios can significantly skew test results.
- Excessive EDTA can lead to shrinkage of erythrocytes, adversely affecting tests like microhematocrit.
Effects of Insufficient & Excessive EDTA
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Insufficient EDTA:
- Caused by overfilling the collection tube.
- Results in the formation of clots in the sample.
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Excessive EDTA:
- Caused by underfilling or short drawing the collection tube.
- Leads to a decrease in hematocrit (Hct) and erythrocyte sedimentation rate (ESR).
- Can cause degenerative changes in white blood cells (WBCs) and increase mean corpuscular hemoglobin concentration (MCHC).
Venipuncture and Specimen Handling
- The order of draw in venipuncture must be followed to minimize contamination of samples.
- Proper handling of specimens post-collection is essential to ensure validity and reliability of hematological test results.
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Description
Prepare for your Hematology midterm with this quiz focused on anticoagulants and erythropoiesis. Learn key concepts including the use of EDTA as an anticoagulant and its effects on platelet volume. This is essential for any student studying Hematology this semester.
