18 Questions
What can happen to RBCs if tubes are underfilled with EDTA?
RBCs can shrink.
What is the purpose of using sodium citrate in coagulation tests?
To prevent coagulation by binding or chelating calcium.
How is heparin utilized in laboratory tests?
Heparin prevents clotting by inhibiting thrombin formation.
What is the role of yellow stopper tubes in the laboratory?
To prevent coagulation by binding calcium.
Why is EDTA K3 not preferred for some tests?
EDTA K3 can cause cells to shrink.
What is the purpose of potassium oxalate in laboratory tubes?
Potassium oxalate prevents coagulation by precipitating calcium.
What is the purpose of ACD solution in immunohematology tests?
Preserves glucose for up to 3 days and inhibits bacterial growth
What substances are included in tubes used to collect serum specimens?
Silica particles, inert clays like Celite, and clotting factors such as thrombin
How do Silica particles function in serum-separator tubes?
They are clot activators and cause blood to clot within 15 to 30 minutes
What is the role of Thrombin tubes in blood collection?
Clot within 5 minutes with gentle inversions
Explain the function of Thixotropic gel separator in blood collection tubes.
It is an inert substance that separates serum or plasma from other blood components
How does factor 12 (Glass Factor) affect coagulation in plasma?
It can activate coagulation and clotting when in contact with glass
What color is used for a 20-gauge needle according to several large manufacturers?
Yellow
According to OSHA regulations, how should the tube holder with needle attached be disposed of?
As a unit after use and never be removed from the needle and reused.
What does SESIP stand for according to OSHA, in relation to sharp objects with engineered protection?
Sharp with Engineered Sharps Injury Protection
What can cause premature loss of vacuum in evacuated tubes used for blood collection?
Improper storage, opening the tube, dropping the tube, advancing the tube too far onto the needle before venipuncture, or if the needle bevel becomes partially out of the skin during venipuncture.
Name one type of additive used in tubes for blood collection to prevent clotting.
Clot activators
What does antiglycolytic agents preserve in blood samples?
Sugar
Test your knowledge on hematology testing procedures, including the use of spray-dried EDTA and the importance of proper tube filling. Learn about the significance of Flow Counters in cell size measurement and the interference of microclots in blood typing. Understand the role of anticoagulants like K2 and sodium citrate in preventing coagulation during testing.
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