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Questions and Answers
What is the purpose of evaluating clotting factors in Secondary Hemostasis?
What is the purpose of evaluating clotting factors in Secondary Hemostasis?
Which tube is checked first for clotting formation in the process described?
Which tube is checked first for clotting formation in the process described?
What method is used to check clotting time in tube #3?
What method is used to check clotting time in tube #3?
Why is the Activated Clotting Time considered insensitive to factor deficiencies?
Why is the Activated Clotting Time considered insensitive to factor deficiencies?
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What issue makes the Capillary Tube Method unreliable for producing results?
What issue makes the Capillary Tube Method unreliable for producing results?
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Which method can specify factor deficiency but is insensitive to identifying the exact deficient clotting factor?
Which method can specify factor deficiency but is insensitive to identifying the exact deficient clotting factor?
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What is the purpose of using a capillary tube with a blue mark (without anticoagulant) in a blood clotting test?
What is the purpose of using a capillary tube with a blue mark (without anticoagulant) in a blood clotting test?
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Which clotting factor deficiency cannot be specifically identified using the test described in the text?
Which clotting factor deficiency cannot be specifically identified using the test described in the text?
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What is the primary reason for the obsolescence of the Lee and White whole blood coagulation time method?
What is the primary reason for the obsolescence of the Lee and White whole blood coagulation time method?
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Which factor is NOT part of the common pathway in the laboratory evaluation of intrinsic and common pathways as stated in the text?
Which factor is NOT part of the common pathway in the laboratory evaluation of intrinsic and common pathways as stated in the text?
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What is the activator used in the activated clotting time test described in the text?
What is the activator used in the activated clotting time test described in the text?
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Why are problems related to intrinsic and common pathways often associated with liver diseases according to the text?
Why are problems related to intrinsic and common pathways often associated with liver diseases according to the text?
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Study Notes
Laboratory Evaluation of Secondary Hemostasis
- To evaluate clotting factors (coagulation factors) for Secondary Hemostasis, collect 4 mL of blood and divide it into three tubes.
- Start timing as 1 mL of blood is transferred into tube #3, then 1 mL for tube #2 and tube #1, and incubate.
Clotting Time Methods
- Slide/Drop Method: Useful for children, uses a small amount of blood, and is done by capillary puncture.
- Capillary Tube Method/Dale and Laidlaw's Method: Uses a capillary tube with a blue mark, no anticoagulant; not usually performed due to skin puncture risk.
- Lee and White Whole Blood Coagulation Time: An obsolete method, insensitive to factor deficiencies.
Activated Clotting Time (ACT)
- Used for patients undergoing heparin therapy.
- An activator, diatomaceous earth (12mg diatomide), is used to stimulate clotting factors.
- Samples are incubated at 37℃; normal range: 75-120 secs, and 140-185 secs for patients undergoing heparin therapy.
Laboratory Evaluation of Intrinsic and Common Pathways
- Intrinsic Pathway: Factors XII, XI, IX, VIII, HMWK, PK.
- Common Pathway: Factors X, V, II, I, XIII (XIII not part of the common pathway).
- Problems with intrinsic and common pathways are related to liver diseases since the liver produces clotting factors.
- Affected by circulating anticoagulants (e.g., heparin, which prolongs clotting time) and hypofibrinogenemia (low fibrinogen levels).
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Description
Learn about the laboratory evaluation of secondary hemostasis in hematology, including collecting blood samples and assessing clotting factors. Understand the process of incubating blood samples, evaluating clot formation, and interpreting results for secondary hemostasis.
