Hematocrit Test: Packed Cell Volume (PCV)

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Questions and Answers

Which of the following is the correct definition of hematocrit?

  • The percentage of platelets in the total blood volume.
  • The measure of the ratio of the volume occupied by plasma to the volume of whole blood.
  • The percentage of red blood cells in the total blood volume. (correct)
  • The measure of the ratio of the volume occupied by white blood cells to the volume of whole blood.

In a complete blood count (CBC) test, what other parameters are typically assessed along with hematocrit (PCV)?

  • Hemoglobin, reticulocyte count, red blood cell count, and white blood cell count. (correct)
  • Blood glucose and hormone levels.
  • Coagulation factors and lipid profile.
  • Electrolyte and enzyme levels.

Which of the following is NOT a common reason for ordering a hematocrit test?

  • To evaluate anemia.
  • To screen for dietary deficiencies. (correct)
  • To assess response to treatment.
  • To monitor dehydration.

What does PCV (Packed Cell Volume) specifically measure in a blood sample?

<p>The ratio of red cell volume to total blood volume. (A)</p> Signup and view all the answers

Which of the following methods for estimating hematocrit involves a shorter centrifugation time and better packing of red cells?

<p>Microhematocrit tube method. (A)</p> Signup and view all the answers

A patient's hematocrit level is outside the generally accepted range. Which factor could influence the interpretation of this result?

<p>The laboratory's specific reference ranges. (D)</p> Signup and view all the answers

Which of the following conditions is LEAST likely to be associated with low hematocrit levels?

<p>Dehydration (A)</p> Signup and view all the answers

What condition is most likely indicated by high hematocrit levels?

<p>Dehydration (C)</p> Signup and view all the answers

Why is it important to know if a patient has recently had a blood transfusion before performing a hematocrit test?

<p>Transfusions can affect the accuracy of the hematocrit reading. (A)</p> Signup and view all the answers

What effect might living at a high altitude have on a person's hematocrit levels?

<p>Increased hematocrit due to reduced amounts of oxygen in the air. (C)</p> Signup and view all the answers

Which of the following is important for ensuring the quality and accuracy of hematocrit testing?

<p>Ensuring centrifuges are regularly checked for proper operation. (A)</p> Signup and view all the answers

Incomplete packing during centrifugation can lead to errors in hematocrit testing. What is the primary cause of this?

<p>Insufficient centrifugation. (C)</p> Signup and view all the answers

To avoid errors when reading hematocrit results, which of the following is recommended?

<p>Using a magnifying glass with a reading device. (A)</p> Signup and view all the answers

Hemolysis or clotting of samples can affect hematocrit test results. How should these factors be managed?

<p>Control factors that cause hemolysis and clotting. (D)</p> Signup and view all the answers

How soon after collection should a blood sample for microhematocrit or PCV measurements be centrifuged to ensure the most accurate results?

<p>Within 6 hours. (D)</p> Signup and view all the answers

Which of the following materials is NOT typically required for performing a manual hematocrit test?

<p>Microscope (C)</p> Signup and view all the answers

A newborn has a hematocrit level of 50%. How should this result be interpreted?

<p>Low for newborns (B)</p> Signup and view all the answers

A male patient has a hematocrit level of 38%. What does this indicate?

<p>Low hematocrit. (B)</p> Signup and view all the answers

A female patient has a hematocrit level of 50%. How would you assess this result?

<p>High for females. (A)</p> Signup and view all the answers

If a patient sample is not centrifuged within 6 hours, what error potential could happen?

<p>Both A and B. (C)</p> Signup and view all the answers

Flashcards

Hematocrit (Hct)

The percentage of red blood cells in the total blood volume; also known as packed cell volume (PCV).

Complete Blood Count (CBC)

A comprehensive blood test that includes hematocrit, hemoglobin, red blood cell count, white blood cell count, and other parameters.

Packed Cell Volume (PCV)

A measure of the ratio of the volume occupied by red blood cells to the volume of whole blood.

Microhematocrit Method

The microhematocrit tube method is a popular technique using small tubes, short centrifugation times, and improved red cell packing.

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Wintrobe Hematocrit Method

A method for estimating hematocrit that uses larger tubes.

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Normal Hematocrit Values

Normal hematocrit ranges vary by age and sex. Adult males: 40-54%; Adult females: 36-47%; Newborns: 55-68%.

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Causes of Low Hematocrit

Conditions that can cause low hematocrit levels include bone marrow diseases, chronic inflammation, iron/folate/B12 deficiencies, internal bleeding, hemolytic anemia, kidney failure, leukemia, lymphoma, and sickle cell anemia.

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Causes of High Hematocrit

Conditions that can cause high hematocrit levels include congenital heart disease, dehydration, kidney tumors, lung diseases, and polycythemia.

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Factors Affecting Hct Results

Factors that can affect hematocrit test results include recent blood transfusions, pregnancy (decreased BUN), and living at high altitudes (higher Hct).

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Sources of Error in Hct Testing

Incomplete packing from insufficient centrifugation, incorrect reading, hemolysis or clotting, and delays in processing samples.

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Materials for Hematocrit Testing

Capillary tubes, centrifuges, clay sealing, hematocrit readers, and micropipettes.

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Study Notes

  • A hematocrit test, also known as packed cell volume (PCV) or Hct, measures the percentage of red blood cells in the total blood volume.
  • Red blood cells are essential for overall health
  • The hematocrit test may be performed if there are too few or too many red blood cells.
  • A hematocrit (PCV) test is included as part of a complete blood count (CBC).
  • Other tests in a CBC include hemoglobin, reticulocyte count, red blood cell count, and white blood cell count.

Purpose of Hematocrit Test

  • Helps diagnose medical conditions and monitors the body's response to treatments
  • Commonly used to test for anemia, leukemia, dehydration, and dietary deficiencies

How Hematocrit is Determined

  • PCV measures the ratio of red cell volume to whole blood volume in capillary, venous, or arterial blood samples.
  • It is an easily obtained measure for detecting anemia or polycythemia.

Methods to Estimate Hematocrit

  • Microhematocrit tube method: a popular micro-method that offers shorter centrifugation time and better red cell packing
  • Wintrobe hematocrit method: a macro method
  • The normal hematocrit levels depend on gender and age
  • Children ages 15 and under have a separate set of ranges

Normal Hematocrit Values

  • Males: 40%-54%
  • Females: 36%-47%
  • Newborns: 55-68%

Low Hematocrit Levels

  • May indicate bone marrow diseases, chronic inflammatory disease, or nutrient deficiencies (iron, folate, or vitamin B-12)
  • Can be a sign of internal bleeding, hemolytic anemia, kidney failure, leukemia, lymphoma, or sickle cell anemia

High Hematocrit Levels

  • May indicate congenital heart disease, dehydration, or kidney tumor
  • Can be a sign of lung diseases or polycythemia

Clinical Considerations for Hematocrit Test

  • Inform the healthcare provider if there was a recent blood transfusion or if pregnancy occurs
  • Pregnancy can lower blood urea nitrogen (BUN) levels due to increased bodily fluids
  • Living at high altitudes can elevate hematocrit levels due to reduced oxygen in the air

Sources of Error and Quality Control

  • Incomplete packing of blood cells can result from insufficient centrifugation; centrifuges should be checked regularly
  • Incorrect reading of results can be avoided by using a magnifying glass with the reading device
  • Hemolysis or clotting of samples should be controlled
  • Blood samples for microhematocrit or PCV measurements should be centrifuged within 6 hours of collection

Materials Needed

  • Capillary tubes
  • Centrifuge
  • Clay sealing
  • Hematocrit reader
  • Micropipettes

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