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Questions and Answers
What is the recommended depth to immerse the tip below the top of the liquid?
How many times should the tip be prewetted for accurate delivery of the desired volume?
Where should the tip be kept clear from while immersing it in the liquid?
What should be done for every sample of a different composition?
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What happens if you don't prewet the tip 3 times?
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How can you avoid restricting the uptake of your liquid sample?
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Why is it important to use a fresh tip for every different sample?
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What is the purpose of aspirating and expelling the tip 3 times?
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What is the consequence of immersing the tip too deep in the liquid?
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What should be the handling procedure if you switch to a sample with a different composition?
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Study Notes
Handling Micropipettes
- Micropipettes are precision instruments that are expensive (about $200 each) and easily broken, but good technique can make them last without repairs.
- Store micropipettes upright on a rack when not in use.
- Use smooth action when handling micropipettes, avoiding snapping noises.
Selecting the Correct Micropipettor
- Select the correct micropipettor for the job by checking the size range on the barrel.
Preparing to Use a Micropipette
- Choose the correct micropipette and set it to the desired volume.
- Do not exceed the micropipette's volume range, as this can break it.
- Always put a tip on the pipette, using a different size tip for each size pipette.
Scales on Micropipettes
- White top micropipettes have a scale of 0.5-10 μL, with markings at 1 μL, 10 μL, and 100 μL.
- Yellow top micropipettes have a scale of 10-100 μL, with two scales: one from 10 μL to 0 μL and one from 10 to 0.
- Blue top micropipettes have a scale of 100-1000 μL, with markings at 100 μL, 200 μL, ..., 1000 μL.
Using a Micropipette
- To draw up a sample, depress the micropipette to the first stop, immerse the tip in the liquid, release slowly, and the liquid will be drawn into the tip.
- Withdraw the tip by pulling the pipette straight out.
- To deliver the sample, insert the tip into a container, depress the push-button to the first stop, pause for 1 second, then depress to the second stop and pause again for 1 second.
Fine Points on the Process
- Prewet the tip by aspirating and expelling the liquid three times for accurate delivery of the desired volume.
- Immerse the tip about 2-6 mm below the top of the liquid, avoiding the sides and bottom of the container.
- Use a fresh tip for every sample of a different composition.
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Description
Learn how to properly handle and select micropipettes in a laboratory setting to avoid damage and ensure accurate results.
