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Laboratory Techniques: Micropipettes and Spectrophotometers

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Laboratory Techniques: Micropipettes and Spectrophotometers

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Questions and Answers

What is the primary function of a spectrophotometer?

To determine the pH of a solution

To measure the volume of a solution

To measure the intensity of light absorbed by a solution (correct)

To count the number of cells in a solution

Which volume range is typically associated with micropipettes?

1 to 1000 μL (correct)

1 to 1000 nL

1 to 1000 pL

1 to 1000 mL

What is a key advantage of using automatic micropipettes compared to glass pipettes?

They offer more precise volume measurements and are safer to handle (correct)

They have a fixed volume for a specific usage

They are made of glass and are therefore more accurate

They are easier to clean and reuse

How do users select the delivery volume when using an automatic micropipettor?

<p>By adjusting the settings on the device</p> Signup and view all the answers

What is a crucial maintenance step for micropipettes to ensure accurate results?

<p>Periodically checking their calibration</p> Signup and view all the answers

Study Notes

Spectrophotometer

Measures the amount of photons (light intensity) absorbed by a sample solution.

Micropipette

Used for dispensing volumes between 1 and 1000 µL.
More accurate and safer than glass pipettes.

Pipetting Technique Using Automatic Pipettes

Automatic micropipettes are common in labs for rapid, repeated measurements.
They deliver preset volumes accurately and reproducibly.

Automatic Micropipettors

Piston-operated devices for delivering small volumes.
Allow precise delivery of reagents and specimens.
Volume settings are adjustable.
Deliver volumes from 0.5 to 1000 µL.
Regular calibration is necessary.

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Description

This quiz covers essential laboratory techniques, focusing on the use of micropipettes, spectrophotometers, and automatic micropipettors. Participants will learn about their functions, advantages, and proper pipetting techniques for accurate measurements. Test your knowledge of these critical tools in scientific research and analysis.