Podcast
Questions and Answers
What is the primary function of a spectrophotometer?
What is the primary function of a spectrophotometer?
- To determine the pH of a solution
- To measure the volume of a solution
- To measure the intensity of light absorbed by a solution (correct)
- To count the number of cells in a solution
Which volume range is typically associated with micropipettes?
Which volume range is typically associated with micropipettes?
- 1 to 1000 μL (correct)
- 1 to 1000 nL
- 1 to 1000 pL
- 1 to 1000 mL
What is a key advantage of using automatic micropipettes compared to glass pipettes?
What is a key advantage of using automatic micropipettes compared to glass pipettes?
- They offer more precise volume measurements and are safer to handle (correct)
- They have a fixed volume for a specific usage
- They are made of glass and are therefore more accurate
- They are easier to clean and reuse
How do users select the delivery volume when using an automatic micropipettor?
How do users select the delivery volume when using an automatic micropipettor?
What is a crucial maintenance step for micropipettes to ensure accurate results?
What is a crucial maintenance step for micropipettes to ensure accurate results?
Flashcards
What is a spectrophotometer?
What is a spectrophotometer?
An instrument that measures how much light is absorbed by a solution.
What is a micropipette?
What is a micropipette?
A type of pipette that dispenses very small volumes (between 1 and 1000 microliters).
What are automatic micropipettors?
What are automatic micropipettors?
Micropipettes that are automatic or semi-automatic, allowing for rapid and repetitive dispensing of liquids in set volumes.
What is pipetting technique using automatic pipettes?
What is pipetting technique using automatic pipettes?
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Why is micropipette calibration important?
Why is micropipette calibration important?
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Study Notes
Spectrophotometer
- Measures the amount of photons (light intensity) absorbed by a sample solution.
Micropipette
- Used for dispensing volumes between 1 and 1000 µL.
- More accurate and safer than glass pipettes.
Pipetting Technique Using Automatic Pipettes
- Automatic micropipettes are common in labs for rapid, repeated measurements.
- They deliver preset volumes accurately and reproducibly.
Automatic Micropipettors
- Piston-operated devices for delivering small volumes.
- Allow precise delivery of reagents and specimens.
- Volume settings are adjustable.
- Deliver volumes from 0.5 to 1000 µL.
- Regular calibration is necessary.
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