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Haematocrit Value and Packed Cell Volume

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Questions and Answers

What is the purpose of centrifugation in measuring Packed Cell Volume?

To mix the blood cells with the plasma
To increase the volume of the blood sample
To decrease the volume of the blood sample
To separate blood cells from plasma based on difference in specific gravity (correct)

What is the purpose of using a capillary tube or Wintrobe tube containing anticoagulant?

To mix the blood cells with the plasma
To increase the volume of the blood sample
To prevent the blood from clotting (correct)
To facilitate the flow of blood into the tube

What is the formula to calculate Packed Cell Volume (PCV)?

(R.B.Cs.volume / total blood volume) × 100 (correct)
(R.B.Cs.volume × total blood volume) / 100
(R.B.Cs.volume + total blood volume) / 100
(total blood volume / R.B.Cs.volume) × 100

What is the specific gravity of R.B.Cs?

1090 (A) Signup and view all the answers

What is the centrifugation rate and duration used in measuring Packed Cell Volume?

3000 cycles/min for 3.5 minutes (A) Signup and view all the answers

What is the last step in determining the Packed Cell Volume?

Determining the PCV from the scale on the apparatus (D) Signup and view all the answers

What is the purpose of adding anticoagulant to the blood sample in a Wintrobe tube?

To prevent blood clotting (B) Signup and view all the answers

What is the formula to calculate packed cell volume (PCV)?

(RBCs volume / total blood sample volume) x 100 (B) Signup and view all the answers

Why does the value of PCV vary in the same subject depending on the source of the blood sample?

Due to plasma skimming in large vessels (A) Signup and view all the answers

What is the difference in PCV between venous and arterial blood?

3% higher in venous blood (C) Signup and view all the answers

What is one of the limitations of using PCV in diagnosis?

It is not diagnostic alone for anemia (D) Signup and view all the answers

What is the formula to calculate total blood volume when RBCs volume is known?

(RBCs vol. x 100) / PCV (A) Signup and view all the answers

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