12 Questions
What is the purpose of centrifugation in measuring Packed Cell Volume?
To separate blood cells from plasma based on difference in specific gravity
What is the purpose of using a capillary tube or Wintrobe tube containing anticoagulant?
To prevent the blood from clotting
What is the formula to calculate Packed Cell Volume (PCV)?
(R.B.Cs.volume / total blood volume) × 100
What is the specific gravity of R.B.Cs?
1090
What is the centrifugation rate and duration used in measuring Packed Cell Volume?
3000 cycles/min for 3.5 minutes
What is the last step in determining the Packed Cell Volume?
Determining the PCV from the scale on the apparatus
What is the purpose of adding anticoagulant to the blood sample in a Wintrobe tube?
To prevent blood clotting
What is the formula to calculate packed cell volume (PCV)?
(RBCs volume / total blood sample volume) x 100
Why does the value of PCV vary in the same subject depending on the source of the blood sample?
Due to plasma skimming in large vessels
What is the difference in PCV between venous and arterial blood?
3% higher in venous blood
What is one of the limitations of using PCV in diagnosis?
It is not diagnostic alone for anemia
What is the formula to calculate total blood volume when RBCs volume is known?
(RBCs vol. x 100) / PCV
Learn about the concept of haematocrit value, packed cell volume, and how to calculate it. Understand the importance of separating blood cells from plasma by centrifugation.
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