10 Questions
What is the primary purpose of Gram staining?
To differentiate bacteria into Gram-positive and Gram-negative
Who introduced the Gram staining technique?
Hans Christian Gram
What causes the retention of the primary stain in Gram-positive bacteria?
Thick layer of peptidoglycan in the cell wall
What happens to the cell wall of Gram-positive bacteria during the decolorization step?
It dehydrates and shrinks
What prevents the ethanol from removing the Crystal Violet-Iodine complex in Gram-positive bacteria?
Thick layer of peptidoglycan in the cell wall
What is the primary purpose of Gram staining?
To differentiate bacteria into Gram-positive and Gram-negative
Who introduced the Gram staining technique?
Hans Christian Gram
What component of gram-positive bacteria's cell walls contributes to their retention of the primary stain?
Protein-sugar complexes
What is the role of the mordant in Gram staining?
To fix the primary stain to the bacterial cells
What happens to the cell walls of gram-positive bacteria when decolorized by alcohol?
They dehydrate and shrink, closing the pores in the cell wall
Test your knowledge of Gram's staining technique with this quiz. Learn about the principles and procedures involved in differentiating bacteria into Gram-positive and Gram-negative.
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