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Questions and Answers
What is the primary purpose of Gram staining?
What is the primary purpose of Gram staining?
- To determine bacterial motility
- To differentiate bacteria into Gram-positive and Gram-negative (correct)
- To identify bacterial species
- To assess bacterial metabolic activity
Who introduced the Gram staining technique?
Who introduced the Gram staining technique?
- Hans Christian Gram (correct)
- Robert Koch
- Alexander Fleming
- Louis Pasteur
What causes the retention of the primary stain in Gram-positive bacteria?
What causes the retention of the primary stain in Gram-positive bacteria?
- Presence of capsule around the cell wall
- Thick layer of peptidoglycan in the cell wall (correct)
- High lipid content in the cell wall
- Low protein content in the cell wall
What happens to the cell wall of Gram-positive bacteria during the decolorization step?
What happens to the cell wall of Gram-positive bacteria during the decolorization step?
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What prevents the ethanol from removing the Crystal Violet-Iodine complex in Gram-positive bacteria?
What prevents the ethanol from removing the Crystal Violet-Iodine complex in Gram-positive bacteria?
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What is the primary purpose of Gram staining?
What is the primary purpose of Gram staining?
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Who introduced the Gram staining technique?
Who introduced the Gram staining technique?
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What component of gram-positive bacteria's cell walls contributes to their retention of the primary stain?
What component of gram-positive bacteria's cell walls contributes to their retention of the primary stain?
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What is the role of the mordant in Gram staining?
What is the role of the mordant in Gram staining?
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What happens to the cell walls of gram-positive bacteria when decolorized by alcohol?
What happens to the cell walls of gram-positive bacteria when decolorized by alcohol?
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