Gram-Negative Biochemical Tests Quiz
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Questions and Answers

Explain the specific learning outcomes of the exercise described in the text.

The specific learning outcomes include performing biochemical tests under the gram negative algorithm, interpreting the reaction for each biochemical test, and identifying the organism based on the result of the biochemical test.

What carbohydrates are present in Triple Sugar Iron Test (TSI) and their respective percentages?

Glucose (0.1%), sucrose (1%), and lactose (1%) are the carbohydrates present in TSI.

What are the additional components present in TSI apart from the carbohydrates?

In addition to the carbohydrates, TSI also contains beef extract, yeast extract, and peptones.

How is TSI different from Kligler's iron agar in terms of carbohydrate composition?

<p>TSI contains three carbohydrates: glucose (0.1%), sucrose (1%), and lactose (1%), while Kligler's iron agar contains only two carbohydrates: glucose (0.1%) and lactose (1%).</p> Signup and view all the answers

What are the recommended learning resources for this exercise?

<p>The recommended learning resources include writing materials, drawing and coloring materials, textbook/web references.</p> Signup and view all the answers

Flashcards

TSI Carbohydrates

TSI contains glucose (0.1%), sucrose (1%), and lactose (1%).

TSI Additional Components

TSI also contains beef extract, yeast extract, and peptones.

TSI vs. Kligler's

TSI has glucose, sucrose, and lactose; Kligler's has glucose and lactose.

Gram-Negative Algorithm

Procedure for identifying Gram-negative bacteria using biochemical tests.

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Biochemical Tests

Tests used to determine specific characteristics of microorganisms.

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Learning Outcomes

Perform, interpret, and identify organisms using biochemical tests.

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Sucrose Percentage TSI

1% in Triple Sugar Iron agar.

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Glucose Percentage TSI

0.1% in Triple Sugar Iron agar.

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Lactose Percentage TSI

1% in Triple Sugar Iron agar.

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Kligler's Iron Agar Carbohydrates

Glucose and lactose are used.

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Learning Resources

Materials like writing tools, diagrams, and references.

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Biochemical Test Interpretation

Determine bacteria characteristics by analyzing test reactions.

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Gram-Negative Identification

Identify bacteria using biochemical test outcomes.

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Triple Sugar Iron (TSI) Test

Biochemical test for identifying bacteria types.

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Learning Outcomes (Specific)

Includes identification of bacteria using results of biochemical tests.

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Kligler's Iron Agar

Biochemical test with fewer carbohydrates compared to TSI, specifically using glucose and lactose.

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Study Notes

University of the Immaculate Conception - Medical Technology/Medical Laboratory Science

Learning Activity No. 13 - Biochemical Test for Gram Negative Organism

  • Specific Learning Outcomes:
    • Perform different biochemical tests under the gram negative algorithm
    • Interpret the reaction for each biochemical test
    • Identify the organism based on the result of the biochemical test

Time Allotment and Learning Resources

  • Time Allotment: 1 hour and 30 minutes
  • Learning Resources:
    • Writing materials
    • Drawing and Coloring Materials
    • Textbook/Web references

Triple Sugar Iron (TSI) Test

  • Composition:
    • Three carbohydrates: glucose (0.1%), sucrose (1%), and lactose (1%)
    • Beef extract
    • Yeast extract
    • Peptones
  • Similarity to Kligler's Iron Agar:
    • Except that Kligler's Iron Agar contains only two carbohydrates: glucose (0.1%) and lactose (1%)

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Description

Test your knowledge of biochemical tests for identifying Gram-negative organisms with this learning activity. Challenge yourself to perform various tests and interpret their reactions to enhance your understanding of Medical Technology/Medical Laboratory Science.

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