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Questions and Answers
Explain the specific learning outcomes of the exercise described in the text.
The specific learning outcomes include performing biochemical tests under the gram negative algorithm, interpreting the reaction for each biochemical test, and identifying the organism based on the result of the biochemical test.
What carbohydrates are present in Triple Sugar Iron Test (TSI) and their respective percentages?
Glucose (0.1%), sucrose (1%), and lactose (1%) are the carbohydrates present in TSI.
What are the additional components present in TSI apart from the carbohydrates?
In addition to the carbohydrates, TSI also contains beef extract, yeast extract, and peptones.
How is TSI different from Kligler's iron agar in terms of carbohydrate composition?
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What are the recommended learning resources for this exercise?
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Study Notes
University of the Immaculate Conception - Medical Technology/Medical Laboratory Science
Learning Activity No. 13 - Biochemical Test for Gram Negative Organism
- Specific Learning Outcomes:
- Perform different biochemical tests under the gram negative algorithm
- Interpret the reaction for each biochemical test
- Identify the organism based on the result of the biochemical test
Time Allotment and Learning Resources
- Time Allotment: 1 hour and 30 minutes
- Learning Resources:
- Writing materials
- Drawing and Coloring Materials
- Textbook/Web references
Triple Sugar Iron (TSI) Test
- Composition:
- Three carbohydrates: glucose (0.1%), sucrose (1%), and lactose (1%)
- Beef extract
- Yeast extract
- Peptones
- Similarity to Kligler's Iron Agar:
- Except that Kligler's Iron Agar contains only two carbohydrates: glucose (0.1%) and lactose (1%)
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Description
Test your knowledge of biochemical tests for identifying Gram-negative organisms with this learning activity. Challenge yourself to perform various tests and interpret their reactions to enhance your understanding of Medical Technology/Medical Laboratory Science.
