Biochemical & Antimicrobial Susceptibility Test for Gram Positive Bacteria

Study Notes

Biochemical Tests Overview

Biochemical tests identify or confirm bacterial species through metabolic activities.
Key factors in identification include differences in protein and fat metabolism, carbohydrate metabolism, enzyme production, and compound utilization.

Identification Tests for Gram Positive Bacteria

Various tests are utilized specifically for identifying Gram positive bacteria, including:
- Catalase Test
- Mannitol Salt Agar (MSA)
- Coagulase Test
- CAMP Test
- DNase Test
- Nitrate Reduction Test
- Taxos A (Bacitracin Sensitivity Testing)
- Taxos P (Optochin Sensitivity Testing)
- Spirit Blue Lipase Test
- API System Test
- Automated Biochemical ID systems (e.g., Vitek-2 Compact System)

Catalase Test Details

The catalase test detects the presence of the enzyme catalase, which detoxifies hydrogen peroxide.
Procedure:
- Apply 2-3 drops of hydrogen peroxide (H2O2) on a clean slide.
- Mix bacterial colonies with the drops of H2O2.
- Observe for results.
Results Interpretation:
- Positive Reaction: Bubbling indicates catalase production.
- Negative Reaction: No bubbling indicates absence of catalase.

Description

Test your knowledge on biochemical tests and antimicrobial susceptibility tests conducted for the identification and confirmation of different gram positive bacterial species. This quiz is based on practical medical microbiology concepts and procedures.