Podcast
Questions and Answers
In the Casein Hydrolysis Test, protein breakdown results in a clear zone around the ______.
In the Casein Hydrolysis Test, protein breakdown results in a clear zone around the ______.
bacteria
Urea Hydrolysis involves the breakdown of urea into ammonia and carbon dioxide, resulting in a ______ color change.
Urea Hydrolysis involves the breakdown of urea into ammonia and carbon dioxide, resulting in a ______ color change.
pink
Gelatin Hydrolysis results in a liquified media due to the action of ______ on gelatin.
Gelatin Hydrolysis results in a liquified media due to the action of ______ on gelatin.
gelatinase
In the Citrate Utilization Test, the color of the Simmons Citrate media changes from green to ______.
In the Citrate Utilization Test, the color of the Simmons Citrate media changes from green to ______.
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Tryptophan Hydrolysis results in a pink color in the presence of ______ from using the Kivacs reagent.
Tryptophan Hydrolysis results in a pink color in the presence of ______ from using the Kivacs reagent.
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Carbohydrate fermentation in a Durham tube results in a yellow color change with ______ due to acid gas production.
Carbohydrate fermentation in a Durham tube results in a yellow color change with ______ due to acid gas production.
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Study Notes
Casein Hydrolysis Test
- Uses skim milk plate medium
- Detects bacteria that produce the enzyme caseinase
- Caseinase breaks down casein (milk protein)
- Clear zone around bacterial growth indicates casein hydrolysis
Urea Hydrolysis Test
- Uses urea slant medium
- Detects bacteria that produce the enzyme urease
- Urease breaks down urea into ammonia and carbon dioxide
- Ammonia raises pH, turning the medium pink
Gelatin Hydrolysis Test
- Uses nutrient gelatin media
- Detects bacteria that produce the enzyme gelatinase
- Gelatinase breaks down gelatin into amino acids and polypeptides
- Positive result: medium liquefies
Catalase Test
- Uses TSA slant media
- Detects bacteria that produce the enzyme catalase
- Catalase breaks down hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2)
- Bubbles form in the media, indicating a positive result
Citrate Utilization Test
- Uses Simmons Citrate media
- Detects bacteria that can use citrate as a sole carbon source
- Positive result causes a color change from green to blue
Tryptophan Hydrolysis Test
- Uses BBL dry slide
- Detects bacteria that produce the enzyme tryptophanase
- Tryptophanase breaks down tryptophan into indole, ammonia, and pyruvate
- Kovács reagent added to detect indole (pink color)
Carbohydrate Fermentation Test
- Uses Durham tube
- Detects bacteria that ferment a specific carbohydrate
- Bacteria produce acid and/or gas as byproducts of fermentation
- Positive result: color change (usually yellow) and gas bubbles in the Durham tube.
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Description
This quiz covers various biochemical tests used for bacterial identification, including casein hydrolysis, urea hydrolysis, gelatin hydrolysis, catalase, and citrate utilization tests. Each test helps in detecting specific enzymes produced by bacteria. Test your knowledge on these essential microbiological procedures!
