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Questions and Answers
What happens to crystal violet in Gram-negative bacteria during Gram staining?
What happens to crystal violet in Gram-negative bacteria during Gram staining?
What is the primary component of the peptidoglycan layer in Gram-negative bacteria?
What is the primary component of the peptidoglycan layer in Gram-negative bacteria?
What is the function of the capsule in Gram-negative bacteria?
What is the function of the capsule in Gram-negative bacteria?
What is a characteristic of the cell wall of Gram-negative bacteria?
What is a characteristic of the cell wall of Gram-negative bacteria?
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What is a component of the outer membrane of Gram-negative bacteria?
What is a component of the outer membrane of Gram-negative bacteria?
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What traps crystal violet in Gram-positive bacteria?
What traps crystal violet in Gram-positive bacteria?
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What is the main component of the cell wall in Gram-positive bacteria?
What is the main component of the cell wall in Gram-positive bacteria?
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What is the function of the capsule in bacteria?
What is the function of the capsule in bacteria?
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What is the purpose of Gram staining?
What is the purpose of Gram staining?
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What is the outermost layer of Gram-negative bacteria?
What is the outermost layer of Gram-negative bacteria?
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What is the main component of the cell wall in Gram-positive bacteria?
What is the main component of the cell wall in Gram-positive bacteria?
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What is the function of the capsule in many prokaryotes?
What is the function of the capsule in many prokaryotes?
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What is the typical size of the Gram-staining unit?
What is the typical size of the Gram-staining unit?
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What is the outermost layer of the Gram-negative bacterial cell wall?
What is the outermost layer of the Gram-negative bacterial cell wall?
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What is the main component of the Gram-negative bacterial cell wall?
What is the main component of the Gram-negative bacterial cell wall?
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What is the primary function of the peptidoglycan layer in Gram-positive bacteria?
What is the primary function of the peptidoglycan layer in Gram-positive bacteria?
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Study Notes
Gram Staining
- Gram-positive bacteria: peptidoglycan traps crystal violet, resulting in a purple color.
- Gram-negative bacteria: crystal violet is easily rinsed away, revealing a red color.
Cell-Surface Structures of Bacteria
- Cell wall consists of peptidoglycan (also known as murein) layer.
- Outer membrane is present in Gram-negative bacteria.
- Cell wall is 10 μm in thickness.
- Capsule: a polysaccharide or protein layer that covers many prokaryotes, enabling them to adhere to their substrate or to other individuals in a colony.
Key Differences between Gram-Positive and Gram-Negative Bacteria
- Presence of outer membrane: Gram-negative bacteria have an outer membrane, while Gram-positive bacteria do not.
- Peptidoglycan layer: Gram-positive bacteria have a thick peptidoglycan layer, while Gram-negative bacteria have a thin peptidoglycan layer.
- Lipopolysaccharide: Gram-negative bacteria have a lipopolysaccharide layer in their outer membrane, which is not present in Gram-positive bacteria.
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Description
This quiz deals with the staining process of gram-negative bacteria, where crystal violet is easily rinsed away, revealing red dye. Understand the characteristics and identification of gram-negative bacteria.
