Gram-Negative Bacteria Staining

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Questions and Answers

What happens to crystal violet in Gram-negative bacteria during Gram staining?

  • It is retained in the cell wall
  • It changes to a red color
  • It is easily rinsed away (correct)
  • It prevents the uptake of the red dye

What is the primary component of the peptidoglycan layer in Gram-negative bacteria?

  • Omps
  • Peptidoglycan (correct)
  • Lipopolysaccharides
  • Teichoic acids

What is the function of the capsule in Gram-negative bacteria?

  • To anchor the flagella
  • To provide structural support
  • To facilitate nutrient uptake
  • To protect against phagocytosis (correct)

What is a characteristic of the cell wall of Gram-negative bacteria?

<p>Thin peptidoglycan layer (D)</p> Signup and view all the answers

What is a component of the outer membrane of Gram-negative bacteria?

<p>Porins (C)</p> Signup and view all the answers

What traps crystal violet in Gram-positive bacteria?

<p>Peptidoglycan (B)</p> Signup and view all the answers

What is the main component of the cell wall in Gram-positive bacteria?

<p>Peptidoglycan (D)</p> Signup and view all the answers

What is the function of the capsule in bacteria?

<p>To hinder phagocytosis (D)</p> Signup and view all the answers

What is the purpose of Gram staining?

<p>To determine the type of bacterial cell wall (C)</p> Signup and view all the answers

What is the outermost layer of Gram-negative bacteria?

<p>Outer membrane (C)</p> Signup and view all the answers

What is the main component of the cell wall in Gram-positive bacteria?

<p>Peptidoglycan (A)</p> Signup and view all the answers

What is the function of the capsule in many prokaryotes?

<p>To enable prokaryotes to adhere to their substrate or to other individuals in a colony (C)</p> Signup and view all the answers

What is the typical size of the Gram-staining unit?

<p>10 μm (C)</p> Signup and view all the answers

What is the outermost layer of the Gram-negative bacterial cell wall?

<p>Outer membrane (C)</p> Signup and view all the answers

What is the main component of the Gram-negative bacterial cell wall?

<p>Lipopolysaccharide (A)</p> Signup and view all the answers

What is the primary function of the peptidoglycan layer in Gram-positive bacteria?

<p>To provide structural support to the cell (D)</p> Signup and view all the answers

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Study Notes

Gram Staining

  • Gram-positive bacteria: peptidoglycan traps crystal violet, resulting in a purple color.
  • Gram-negative bacteria: crystal violet is easily rinsed away, revealing a red color.

Cell-Surface Structures of Bacteria

  • Cell wall consists of peptidoglycan (also known as murein) layer.
  • Outer membrane is present in Gram-negative bacteria.
  • Cell wall is 10 μm in thickness.
  • Capsule: a polysaccharide or protein layer that covers many prokaryotes, enabling them to adhere to their substrate or to other individuals in a colony.

Key Differences between Gram-Positive and Gram-Negative Bacteria

  • Presence of outer membrane: Gram-negative bacteria have an outer membrane, while Gram-positive bacteria do not.
  • Peptidoglycan layer: Gram-positive bacteria have a thick peptidoglycan layer, while Gram-negative bacteria have a thin peptidoglycan layer.
  • Lipopolysaccharide: Gram-negative bacteria have a lipopolysaccharide layer in their outer membrane, which is not present in Gram-positive bacteria.

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