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Questions and Answers
What is the advantage of using the interpolation method in graded response assays?
What is the advantage of using the interpolation method in graded response assays?
- Less time-consuming and reliable compared to other bioassay methods
- Involves no statistical data and many calculations (correct)
- Requires multiple concentrations in the curve
- Highly dependent on the dose range
How is the sensitivity of tissue first determined in bioassays?
How is the sensitivity of tissue first determined in bioassays?
- By plotting a dose-response curve with a known agonist (correct)
- By extending the dose range to include more data points
- By diluting the sample after the reaction is read
- By calculating the unknown concentrations directly
What should be done if the drug response does not fall within the dose range in bioassays?
What should be done if the drug response does not fall within the dose range in bioassays?
- Calculate the unknown concentrations using linear regression
- Use statistical data for analysis
- Dilute the sample after the reaction is read
- Extend the dose range by including more concentrations (correct)
What is a key requirement to calculate unknown concentrations using the linear regression method in bioassays?
What is a key requirement to calculate unknown concentrations using the linear regression method in bioassays?
Why is it important not to dilute the sample after the reaction is read in bioassays?
Why is it important not to dilute the sample after the reaction is read in bioassays?
What role does extending the dose range play in interpreting dose response plots?
What role does extending the dose range play in interpreting dose response plots?
In bioassays, what is the focus of a direct assay?
In bioassays, what is the focus of a direct assay?
What is the main limitation of a direct assay?
What is the main limitation of a direct assay?
In an indirect assay, what is the focus of the analysis?
In an indirect assay, what is the focus of the analysis?
How is the relative potency of a test sample determined in an indirect assay?
How is the relative potency of a test sample determined in an indirect assay?
What does the equation 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛𝑜𝑓 𝑢𝑛𝑘𝑛𝑜𝑤𝑛 = 𝑑𝑜𝑠𝑒𝑟𝑒𝑠𝑡 × 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑 represent in bioassays?
What does the equation 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛𝑜𝑓 𝑢𝑛𝑘𝑛𝑜𝑤𝑛 = 𝑑𝑜𝑠𝑒𝑟𝑒𝑠𝑡 × 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑 represent in bioassays?
What aspect of bioassays is highlighted by the statement 'every dose there’s a response'?
What aspect of bioassays is highlighted by the statement 'every dose there’s a response'?
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