12 Questions
What is the advantage of using the interpolation method in graded response assays?
Involves no statistical data and many calculations
How is the sensitivity of tissue first determined in bioassays?
By plotting a dose-response curve with a known agonist
What should be done if the drug response does not fall within the dose range in bioassays?
Extend the dose range by including more concentrations
What is a key requirement to calculate unknown concentrations using the linear regression method in bioassays?
Having a high and low standard response
Why is it important not to dilute the sample after the reaction is read in bioassays?
To avoid affecting the sensitivity of the tissue
What role does extending the dose range play in interpreting dose response plots?
It helps in calculating unknown concentrations accurately
In bioassays, what is the focus of a direct assay?
Dose
What is the main limitation of a direct assay?
Lack of dosaging options for a dose-ranging study
In an indirect assay, what is the focus of the analysis?
Dose-response relationship
How is the relative potency of a test sample determined in an indirect assay?
Through statistical analysis of the dose-response relation
What does the equation 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛𝑜𝑓 𝑢𝑛𝑘𝑛𝑜𝑤𝑛 = 𝑑𝑜𝑠𝑒𝑟𝑒𝑠𝑡 × 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑 represent in bioassays?
Calculation of unknown concentrations
What aspect of bioassays is highlighted by the statement 'every dose there’s a response'?
Dose-response relationship
Test your knowledge on the principles and methods of graded response assay interpolation. Learn about calculating drug potency, determining test substance response, and using multiple concentrations in assays.
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