Glucose Oxidase Reagent Set Analysis

Glucose (Oxidase) Reagent Set (Phenol Free)

• Intended use: Quantitative determination of total glucose in serum.
• Storage: Dry reagent and standard are stored at 2-8°C prior to reconstitution. Reconstituted reagent is stable for 30 days when stored at 2-8°C.
• Stability: Reagent can be used until expiration date on packaging.
• Specimen collection: Serum samples, free from hemolysis, are recommended. Plasma may not be used if anticoagulants are present.
• Interfering substances: Grossly lipemic or icteric sera may cause false glucose values and require a serum blank correction in the analysis.
• Procedure limitations: Reagent is linear to 500 mg/dL. Samples exceeding 500 mg/dL require 1:1 dilution with water, followed by recalculation of test results.
• Materials required (not provided): Pipettes, test tubes, timer, 37°C heating block/water bath and spectrophotometer.
• Procedure (Manual): Prepare reagent, label tubes (blank, standard, control, patient), pipette 1.0 ml working reagent to all tubes, pre-warm in 37°C heating bath, add 10µl sample to each tube, mix, and incubate at 37°C for 10 minutes, zero spectrophotometer with reagent blank at 500nm, and read absorbances.

Cholesterol Reagent Set (Phenol Free)

• Intended use: Quantitative determination of total cholesterol in human serum.
• Storage/Stability: Stored at refrigerator temperature (2-8°C), ready to use format. When reconstituted, the reagent is stable for 60 days at 2-8°C.
• Specimen collection: Serum, free from hemolysis, is preferred. Samples are stable for 7 days at room temperature or 6 months when frozen.
• Interfering Substances: Fluoride and oxalate can cause false low values. Hemoglobin levels up to 200 mg/dL, and bilirubin levels up to 10 mg/dL do not interfere. Grossly lipemic or icteric specimens require the use of a serum/plasma blank.
• Materials required (not provided): Spectrophotometer, test tubes, pipettes, timer, 37°C heating block.
• Procedure (manual): Pre-warm reagent at 37°C for 2 minutes, add sample, incubate for 10 minutes at 37°C, zero spectrophotometer, and record absorbance at 520 nm.

Triglyceride Reagent

• Intended use: Quantitative determination of triglycerides in serum or plasma.
• Storage: Stored at 2-8°C.
• Specimen collection: Fresh, clear, non-hemolyzed serum from fasting patients is recommended. Stable for 3 days at 2-8°C when refrigerated or 6 months when stored and frozen properly.
• Interferences: Grossly lipemic or icteric samples, and high bilirubin or hemolysis may cause false results. Glycerol in collection tube stoppers may interfere. Avoid using collection tubes with glycerol lubrication.
• Materials required (not provided): Spectrophotometer, test tubes, pipettes, hot bath/incubator (37°C), timer.
• Procedure: Add reagent, add sample, incubate, and measure absorbance at 520nm.

Urea Nitrogen (BUN) Reagent

• Intended use: To determine the level of urea nitrogen in serum.
• Storage: Stored at 2-8°C before reconstitution. Reconstituted reagents are stable for 2 days at room temperature (18-25°C) or 21 days at 2-8°C.
• Specimen collection: Serum is preferred, avoiding hemolysis. Stable for 72 hours when refrigerated, and 8 hours without refrigeration.
• Interferences: Anticoagulants containing fluoride, citrate, or EDTA can inhibit urease and should be avoided. High ammonia levels in water or other substances may cause false elevations and should be avoided.
• Materials required (not provided): Pipettes, test tubes, timer, water, spectrophotometer.
• Procedure (manual): Reconstitute reagent, label tubes, add 1.0 ml of reagent to each tube, pre-heat to 37°C, add sample, incubate for 60 seconds, read absorbance at 340nm.

Creatinine Reagent

• Intended use: Quantitative determination of creatinine in serum.
• Storage: Stored at room temperature(15-30°C). Combined reagent is stable for 1 month.
• Specimen collection: Serum samples. Stable for 24 hours when refrigerated or several months when properly frozen.
• Interferences: Potential interferences from albumin, moderate hemolysis, high levels of icterus or lipemia, or acetoacetate.
• Materials required (not provided): Pipettes, test tubes, 37°C water bath/incubator, spectrophotometer.
• Procedure (manual): Label tubes, add reagent and sample, incubate at 37°C, zero spectrophotometer with reagent blank, read absorbance at 510nm.

Uric Acid Reagent

•  Intended use: To quantify serum uric acid.
• Storage: Stored refrigerated. Do not freeze.
• Specimen collection: Serum is preferred, free from hemolysis. Stable for 3 days refrigerated , or months when frozen.
• Interferences: Bilirubin, ascorbic acid, lipemia.
•  Materials required (not provided): Reagent blank, sample tubes, pipettes, timer, spectrophotometer (with a 37°C heating block/water bath).
• Procedure (manual): Warm reagents in a 37°C water bath, Label tubes, Add reagents, Add samples, Incubate at 37°C for 10 minutes, zero at 510nm, read absorbance.

Direct LDL Cholesterol Reagent

• Intended use: Quantitative determination of LDL cholesterol in serum or plasma.
• Storage/Stability: Liquid reagents, stored at 2 to 8 degrees Celsius
• Materials required (not provided): Automatic chemistry analyzer or spectrophotometer, calibration fluid, LDL controls.
•  Procedure (automated or manual): Automated protocol specified by the instrument manufacturer. Manual protocol includes the use of two reagents, and incubation at 37C

Direct HDL Cholesterol Reagent

• Intended use: Quantitative determination of HDL cholesterol in serum or plasma.
• Storage/Stability: Liquid reagents, stored at 2 to 8 degrees Celsius
• Materials required (not provided): Automated chemistry analyzer or spectrophotometer, calibration fluid, HDL controls.
•  Procedure (automated or manual): Automated protocol specified by the instrument. Manual procedure involves preparation of reagents incubation at 37C, and absorbance readings