Podcast
Questions and Answers
What type of ends does an insert with flanking Bsa1 sites have?
What type of ends does an insert with flanking Bsa1 sites have?
- Blunt ends
- Circular ends
- Sticky ends (correct)
- Flared ends
Which process is used to join multiple sequences like a train in Golden Gate Cloning?
Which process is used to join multiple sequences like a train in Golden Gate Cloning?
- Polymerization
- Ligation (correct)
- Digestion
- Annealing
What method is used for RNA quantification and detection?
What method is used for RNA quantification and detection?
- Gel electrophoresis
- Mass spectrometry
- Western blot
- qRT-PCR (correct)
What are the Bsa1 sites used for in vector construction?
What are the Bsa1 sites used for in vector construction?
What enzyme is essential for the quantitative PCR process?
What enzyme is essential for the quantitative PCR process?
In Golden Gate Cloning, what is the function of divergent Bsa1 sites in the destination vector?
In Golden Gate Cloning, what is the function of divergent Bsa1 sites in the destination vector?
Which technique is used to clone long inserts by assembling multiple DNA fragments together?
Which technique is used to clone long inserts by assembling multiple DNA fragments together?
Which enzymes are used in the Gibson assembly method?
Which enzymes are used in the Gibson assembly method?
What is the potential issue with repeating sequences in the DNA fragments used for Gibson assembly?
What is the potential issue with repeating sequences in the DNA fragments used for Gibson assembly?
Which cloning method allows for the assembly of multiple sequence inserts without generating a single stretch of single-stranded DNA?
Which cloning method allows for the assembly of multiple sequence inserts without generating a single stretch of single-stranded DNA?
What is the unique property of the Type IIS restriction enzymes used in Golden Gate cloning?
What is the unique property of the Type IIS restriction enzymes used in Golden Gate cloning?
Which of the following statements about the Type IIS restriction enzymes used in Golden Gate cloning is true?
Which of the following statements about the Type IIS restriction enzymes used in Golden Gate cloning is true?
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