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Questions and Answers
What is the purpose of step 1 in the chromatin immunoprecipitation experiment?
What is the purpose of step 1 in the chromatin immunoprecipitation experiment?
- To isolate DNA after reverse cross-linking
- To shear chromatin into 200–500 bp DNA fragments
- To precipitate RNA polymerase II
- To cross-link proteins to DNA and proteins to proteins (correct)
In the results from mouse embryonic stem cells, what does the left plot indicate about RNA polymerase II transcription?
In the results from mouse embryonic stem cells, what does the left plot indicate about RNA polymerase II transcription?
- Transcription in the antisense direction only
- Transcription with no specific directionality
- Transcription only in the sense direction
- Transcription in both directions (correct)
What is the purpose of using an anti-RNA polymerase II antibody in step 3 of the experiment?
What is the purpose of using an anti-RNA polymerase II antibody in step 3 of the experiment?
- To isolate DNA after reverse cross-linking
- To shear chromatin into fragments
- To precipitate RNA polymerase II and its linked DNA (correct)
- To sequence DNA using massively parallel sequencing
What is the main outcome of chromatin immunoprecipitation using an antibody to RNA polymerase II?
What is the main outcome of chromatin immunoprecipitation using an antibody to RNA polymerase II?
Which step involves reverse cross-linking and isolating DNA in the chromatin immunoprecipitation experiment?
Which step involves reverse cross-linking and isolating DNA in the chromatin immunoprecipitation experiment?
Why is the sonication step needed in the chromatin immunoprecipitation experiment?
Why is the sonication step needed in the chromatin immunoprecipitation experiment?
What is the purpose of chromatin immunoprecipitation (ChIP) technique?
What is the purpose of chromatin immunoprecipitation (ChIP) technique?
In immuno-fluorescence, what is used to localize antigens in cells?
In immuno-fluorescence, what is used to localize antigens in cells?
What is the main function of immuno-precipitation technique?
What is the main function of immuno-precipitation technique?
Which technique involves a combination of immuno-precipitation, PCR, and DNA sequencing?
Which technique involves a combination of immuno-precipitation, PCR, and DNA sequencing?
What is the purpose of immuno-precipitation in the context of antibody production?
What is the purpose of immuno-precipitation in the context of antibody production?
Which technique is specifically used to detect where a protein associates with the genome?
Which technique is specifically used to detect where a protein associates with the genome?
What does the plot of the number of reads over each position in genome represent?
What does the plot of the number of reads over each position in genome represent?
How are antibodies defined?
How are antibodies defined?
What is the characteristic feature of monoclonal antibodies?
What is the characteristic feature of monoclonal antibodies?
How can polyclonal antibodies be obtained?
How can polyclonal antibodies be obtained?
In antibody production, how can highly specific antibodies against a protein of interest be obtained?
In antibody production, how can highly specific antibodies against a protein of interest be obtained?
What is the purpose of injecting animals with antigens in antibody production?
What is the purpose of injecting animals with antigens in antibody production?
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