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Questions and Answers
Whole genome sequencing allows scientists to determine the entirety of the DNA sequence of an organism's genome at different times.
Whole genome sequencing allows scientists to determine the entirety of the DNA sequence of an organism's genome at different times.
False
Sanger sequencing can read or detect long pieces of DNA, around 500-700 bp/read.
Sanger sequencing can read or detect long pieces of DNA, around 500-700 bp/read.
False
Clone-by-clone mapping involves aligning and merging fragments of a DNA sequence to reconstruct the original structure of the DNA.
Clone-by-clone mapping involves aligning and merging fragments of a DNA sequence to reconstruct the original structure of the DNA.
False
Whole-genome shotgun is a DNA sequencing technique that involves breaking the genome into small fragments and then determining their sequence.
Whole-genome shotgun is a DNA sequencing technique that involves breaking the genome into small fragments and then determining their sequence.
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Bpu10I, Cfr10I, DraII, and HindII are enzymes used in clone-by-clone mapping to cut the vector/clone complex for orientation determination.
Bpu10I, Cfr10I, DraII, and HindII are enzymes used in clone-by-clone mapping to cut the vector/clone complex for orientation determination.
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In gel simulation, choosing 'unlimited cuts' allows the restriction enzyme to cut only once.
In gel simulation, choosing 'unlimited cuts' allows the restriction enzyme to cut only once.
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The MWM (Molecular Weight Marker) helps indicate the size of bands on a gel during DNA sequencing.
The MWM (Molecular Weight Marker) helps indicate the size of bands on a gel during DNA sequencing.
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DNA sequence assembly is a process that involves directly interpreting the entire genome in one step.
DNA sequence assembly is a process that involves directly interpreting the entire genome in one step.
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Short pieces of DNA can be read or detected through Sanger sequencing, typically around 800-900 bp/read.
Short pieces of DNA can be read or detected through Sanger sequencing, typically around 800-900 bp/read.
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Comparison of Forward and Reverse orientations is important when using Bpu10I, Cfr10I, DraII, and HindII enzymes in clone-by-clone mapping.
Comparison of Forward and Reverse orientations is important when using Bpu10I, Cfr10I, DraII, and HindII enzymes in clone-by-clone mapping.
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