Genome Editing Tools Quiz

5 Questions

Match the following DNA repair mechanisms with their descriptions:

Homology-directed repair (HDR) = Results in targeted integration Non-homologous end-joining (NHEJ) = Results in gene disruptions Zinc fingers = Binds at specific sites of DNA ZFNs = Engineered by fusing a non

Match the following terms with their descriptions:

Cys2His2 zinc fingers (ZFs) = Mediates highly conserved interactions with homologous DNA sequences Nuclease-induced DNA Double stranded Breaks (DSBs) = Can be repaired by HDR or NHEJ mechanisms Repair template = Delivered into targeted cells directly in a site-specific manner Zinc-finger domain = Comprises an array of Cys2His2 zinc fingers (ZFs) or called as C2H2 domains

Match the following outcomes with the corresponding DNA repair mechanisms:

Targeted integration = Homology-directed repair (HDR) Gene disruptions = Non-homologous end-joining (NHEJ) Mutation correction or new sequence insertion = Delivering an appropriately designed repair template into targeted cells directly in a site-specific manner Array of Cys2His2 zinc fingers (ZFs) interaction with homologous DNA sequences = Zinc fingers

Match the following DNA repair mechanisms with their applications:

Homology-directed repair (HDR) = Introducing precise mutations by delivering an appropriately designed repair template into targeted cells directly in a site-specific manner Non-homologous end-joining (NHEJ) = Repairing nuclease-induced DNA Double stranded Breaks (DSBs) resulting in gene disruptions Zinc fingers = Transcription factor that binds at specific sites of DNA ZFNs = Engineered to introduce precise mutations by fusing a non

Match the following terms with their roles in DNA repair:

Cys2His2 zinc fingers (ZFs) = Mediating highly conserved interactions with homologous DNA sequences Nuclease-induced DNA Double stranded Breaks (DSBs) = Can be repaired by one of the two major mechanisms: homology-directed repair (HDR) and non-homologous end-joining (NHEJ) Repair template = Introducing precise mutations by delivering it into targeted cells directly in a site-specific manner Zinc-finger domain = Comprising an array of Cys2His2 zinc fingers (ZFs) or called as C2H2 domains

Study Notes

Genome Editing Tools for Cell Manipulation

Genome editing is a method that allows for targeted gene editing by making specific changes to the DNA sequence at desired locations in the genome.

Types of Genome Editing Tools

There are three basic genome editing tools that use nucleases to edit the genome:
- Zinc-finger nucleases (ZFNs)
- Transcription activator-like effector nucleases (TALENs)
- Clustered regularly interspaced short palindromic repeat (CRISPR)–Cas-associated nucleases
These nucleases are designed to induce double-stranded DNA breaks at specific locations in the genome.

Mechanism of Genome Editing

The genome editing process involves making a double-stranded DNA break at a specific location, which can then be repaired by the cell's natural repair machinery.
The editing machinery can be delivered in vitro or in vivo to achieve genome editing.

Applications and Implications of Genome Editing

Genome editing can be used to add, delete, or correct genes and perform other targeted genomic modifications.
However, genome editing also raises security risks and ethical concerns due to the potential for irreversible and permanent changes to the genome.