Podcast
Questions and Answers
Match the following DNA repair mechanisms with their descriptions:
Match the following DNA repair mechanisms with their descriptions:
Homology-directed repair (HDR) = Results in targeted integration Non-homologous end-joining (NHEJ) = Results in gene disruptions Zinc fingers = Binds at specific sites of DNA ZFNs = Engineered by fusing a non
Match the following terms with their descriptions:
Match the following terms with their descriptions:
Cys2His2 zinc fingers (ZFs) = Mediates highly conserved interactions with homologous DNA sequences Nuclease-induced DNA Double stranded Breaks (DSBs) = Can be repaired by HDR or NHEJ mechanisms Repair template = Delivered into targeted cells directly in a site-specific manner Zinc-finger domain = Comprises an array of Cys2His2 zinc fingers (ZFs) or called as C2H2 domains
Match the following outcomes with the corresponding DNA repair mechanisms:
Match the following outcomes with the corresponding DNA repair mechanisms:
Targeted integration = Homology-directed repair (HDR) Gene disruptions = Non-homologous end-joining (NHEJ) Mutation correction or new sequence insertion = Delivering an appropriately designed repair template into targeted cells directly in a site-specific manner Array of Cys2His2 zinc fingers (ZFs) interaction with homologous DNA sequences = Zinc fingers
Match the following DNA repair mechanisms with their applications:
Match the following DNA repair mechanisms with their applications:
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Match the following terms with their roles in DNA repair:
Match the following terms with their roles in DNA repair:
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Study Notes
Genome Editing Tools for Cell Manipulation
- Genome editing is a method that allows for targeted gene editing by making specific changes to the DNA sequence at desired locations in the genome.
Types of Genome Editing Tools
- There are three basic genome editing tools that use nucleases to edit the genome:
- Zinc-finger nucleases (ZFNs)
- Transcription activator-like effector nucleases (TALENs)
- Clustered regularly interspaced short palindromic repeat (CRISPR)–Cas-associated nucleases
- These nucleases are designed to induce double-stranded DNA breaks at specific locations in the genome.
Mechanism of Genome Editing
- The genome editing process involves making a double-stranded DNA break at a specific location, which can then be repaired by the cell's natural repair machinery.
- The editing machinery can be delivered in vitro or in vivo to achieve genome editing.
Applications and Implications of Genome Editing
- Genome editing can be used to add, delete, or correct genes and perform other targeted genomic modifications.
- However, genome editing also raises security risks and ethical concerns due to the potential for irreversible and permanent changes to the genome.
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Description
Test your knowledge of genome editing tools for cell manipulation with this quiz. Explore targeted gene editing, in situ genome editing, and the use of molecular scissors to manipulate DNA. Ideal for those interested in genetic engineering and biotechnology.
