37 Questions
Which method is used to deliver mRNA vaccines?
Using lipid nanoparticles with PEG
What is the limitation of mRNA vaccines?
They require low temperature storage
Which of the following is a limitation of RNA interference (RNAi)?
RNAi can only target mRNA
What is the role of Argonaute in RNA interference (RNAi)?
Argonaute cleaves and degrades mRNA
Which type of small non-coding RNA is specific to the germline?
PIWI-interacting RNAs
What is the function of zinc finger nucleases (ZFNs) in genome editing?
ZFNs make a nonspecific double-strand break
Which of the following is a key component of the CRISPR-Cas9 system?
Cas9 endonuclease and a small RNA
What is the purpose of the PAM sequence in the CRISPR-Cas9 system?
To help Cas9 recognize and cleave the target DNA region
How are knockout organisms created using genome editing techniques?
By rendering a gene of interest non-functional
What was the purpose of injecting embryos with mRNA encoding TALENs in the knockout mice experiment?
To create knockout mice with mutations in specific genes
Which method of genome editing uses zinc finger nucleases to target unique DNA sequences?
Zinc finger nucleases
Which repair mechanism is used by cells to fix double-strand breaks made to DNA during genome editing?
Nonhomologous end joining (NHEJ)
Which genome editing method uses components of a prokaryotic immune system to target almost any desired genetic sequence?
CRISPR-Cas9 system
What is the role of the protospacer adjacent motif (PAM) in genome editing using CRISPR?
To identify the 3' end of the genomic location to be edited
Which of the following is true about CRISPR-Cas9 technology?
It is commonly used in the laboratory to remove DNA and insert a new DNA sequence.
What is the function of the guide RNA in CRISPR-Cas9 technology?
It identifies the target DNA sequence.
What is one possible application of the CRISPR-Cas9 system?
All of the above.
What are some ethical and safety considerations associated with the use of CRISPR-Cas9 technology?
All of the above.
Which method is used to deliver mRNA vaccines?
Lipid nanoparticles
What is the function of zinc finger nucleases (ZFNs) in genome editing?
To target specific DNA sequences
What is one possible application of the CRISPR-Cas9 system?
Studying genetic diseases
Which type of organism was the CRISPR-Cas9 system first observed in?
Bacteria
What does CRISPR stand for?
Clustered Regularly Interspaced Short Palindromic Repeats
What is the function of the Cas9 endonuclease in the CRISPR-Cas9 system?
To recognize and cleave the target DNA region
What is one potential application of genome editing techniques?
Curing genetic disorders
Which enzyme cleaves double-stranded RNA into approximately 20-25 nucleotide-long siRNA?
Dicer
What is the role of Argonaute in RNA interference (RNAi)?
It cleaves and degrades mRNA, preventing translation into protein
What is the function of synthetic siRNAs in gene function analysis?
To silence complementary gene sequences
What is the limitation of RNA interference (RNAi) as a gene silencing technique?
It only targets mRNA, allowing continuous transcription of the DNA sequence
Which of the following is true about the CRISPR-Cas9 system?
It is a DNA editing tool that stands for Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR associated protein 9.
Which of the following is a possible application of the CRISPR-Cas9 system?
All of the above
What is the purpose of the guide RNA in CRISPR-Cas9 technology?
To identify the target DNA sequence
What are some ethical and safety considerations associated with the use of CRISPR-Cas9 technology?
All of the above
Which repair mechanism is used by cells to fix double-strand breaks made to DNA during genome editing?
Nonhomologous end joining (NHEJ)
What is the role of the protospacer adjacent motif (PAM) in genome editing using CRISPR?
It helps guide the Cas9 enzyme to the target DNA sequence
What is one possible application of the CRISPR-Cas9 system?
Creating targeted genetic changes in mammalian cells
Which genome editing method uses components of a prokaryotic immune system to target almost any desired genetic sequence?
CRISPR-Cas9 system
Test your knowledge on genome editing principles and advancements in this quiz. Learn about how engineered nuclease enzymes induce double-strand breaks in DNA and how mutations can be introduced into targeted DNA regions.
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