Genome Editing

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Which method is used to deliver mRNA vaccines?

Random insertion of viral vectors

Using lipid nanoparticles with PEG (correct)

Traditional method of making recombinant gene

Site-directed mutagenesis

What is the limitation of mRNA vaccines?

They have a short shelf life

They are difficult to produce

They require low temperature storage (correct)

They have limited efficacy

Which of the following is a limitation of RNA interference (RNAi)?

RNAi can alter DNA sequences

RNAi can only target mRNA (correct)

RNAi can be used to treat all diseases

RNAi can permanently silence genes

What is the role of Argonaute in RNA interference (RNAi)?

Argonaute cleaves and degrades mRNA Signup and view all the answers

Which type of small non-coding RNA is specific to the germline?

PIWI-interacting RNAs Signup and view all the answers

What is the function of zinc finger nucleases (ZFNs) in genome editing?

ZFNs make a nonspecific double-strand break Signup and view all the answers

Which of the following is a key component of the CRISPR-Cas9 system?

Cas9 endonuclease and a small RNA Signup and view all the answers

What is the purpose of the PAM sequence in the CRISPR-Cas9 system?

To help Cas9 recognize and cleave the target DNA region Signup and view all the answers

How are knockout organisms created using genome editing techniques?

By rendering a gene of interest non-functional Signup and view all the answers

What was the purpose of injecting embryos with mRNA encoding TALENs in the knockout mice experiment?

To create knockout mice with mutations in specific genes Signup and view all the answers

Which method of genome editing uses zinc finger nucleases to target unique DNA sequences?

Zinc finger nucleases Signup and view all the answers

Which repair mechanism is used by cells to fix double-strand breaks made to DNA during genome editing?

Nonhomologous end joining (NHEJ) Signup and view all the answers

Which genome editing method uses components of a prokaryotic immune system to target almost any desired genetic sequence?

CRISPR-Cas9 system Signup and view all the answers

What is the role of the protospacer adjacent motif (PAM) in genome editing using CRISPR?

To identify the 3' end of the genomic location to be edited Signup and view all the answers

Which of the following is true about CRISPR-Cas9 technology?

It is commonly used in the laboratory to remove DNA and insert a new DNA sequence. Signup and view all the answers

What is the function of the guide RNA in CRISPR-Cas9 technology?

It identifies the target DNA sequence. Signup and view all the answers

What is one possible application of the CRISPR-Cas9 system?

All of the above. Signup and view all the answers

What are some ethical and safety considerations associated with the use of CRISPR-Cas9 technology?

All of the above. Signup and view all the answers

Which method is used to deliver mRNA vaccines?

Lipid nanoparticles Signup and view all the answers

What is the function of zinc finger nucleases (ZFNs) in genome editing?

To target specific DNA sequences Signup and view all the answers

What is one possible application of the CRISPR-Cas9 system?

Studying genetic diseases Signup and view all the answers

Which type of organism was the CRISPR-Cas9 system first observed in?

Bacteria Signup and view all the answers

What does CRISPR stand for?

Clustered Regularly Interspaced Short Palindromic Repeats Signup and view all the answers

What is the function of the Cas9 endonuclease in the CRISPR-Cas9 system?

To recognize and cleave the target DNA region Signup and view all the answers

What is one potential application of genome editing techniques?

Curing genetic disorders Signup and view all the answers

Which enzyme cleaves double-stranded RNA into approximately 20-25 nucleotide-long siRNA?

Dicer Signup and view all the answers

What is the role of Argonaute in RNA interference (RNAi)?

It cleaves and degrades mRNA, preventing translation into protein Signup and view all the answers

What is the function of synthetic siRNAs in gene function analysis?

To silence complementary gene sequences Signup and view all the answers

What is the limitation of RNA interference (RNAi) as a gene silencing technique?

It only targets mRNA, allowing continuous transcription of the DNA sequence Signup and view all the answers

Which of the following is true about the CRISPR-Cas9 system?

It is a DNA editing tool that stands for Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR associated protein 9. Signup and view all the answers

Which of the following is a possible application of the CRISPR-Cas9 system?

All of the above Signup and view all the answers

What is the purpose of the guide RNA in CRISPR-Cas9 technology?

To identify the target DNA sequence Signup and view all the answers

What are some ethical and safety considerations associated with the use of CRISPR-Cas9 technology?

All of the above Signup and view all the answers

Which repair mechanism is used by cells to fix double-strand breaks made to DNA during genome editing?

Nonhomologous end joining (NHEJ) Signup and view all the answers

What is the role of the protospacer adjacent motif (PAM) in genome editing using CRISPR?

It helps guide the Cas9 enzyme to the target DNA sequence Signup and view all the answers

What is one possible application of the CRISPR-Cas9 system?

Creating targeted genetic changes in mammalian cells Signup and view all the answers

Which genome editing method uses components of a prokaryotic immune system to target almost any desired genetic sequence?

CRISPR-Cas9 system Signup and view all the answers

Genome Editing

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Questions and Answers

Which method is used to deliver mRNA vaccines?

What is the limitation of mRNA vaccines?

Which of the following is a limitation of RNA interference (RNAi)?

What is the role of Argonaute in RNA interference (RNAi)?

Which type of small non-coding RNA is specific to the germline?

What is the function of zinc finger nucleases (ZFNs) in genome editing?

Which of the following is a key component of the CRISPR-Cas9 system?

What is the purpose of the PAM sequence in the CRISPR-Cas9 system?

How are knockout organisms created using genome editing techniques?

What was the purpose of injecting embryos with mRNA encoding TALENs in the knockout mice experiment?

Which method of genome editing uses zinc finger nucleases to target unique DNA sequences?

Which repair mechanism is used by cells to fix double-strand breaks made to DNA during genome editing?

Which genome editing method uses components of a prokaryotic immune system to target almost any desired genetic sequence?

What is the role of the protospacer adjacent motif (PAM) in genome editing using CRISPR?

Which of the following is true about CRISPR-Cas9 technology?

What is the function of the guide RNA in CRISPR-Cas9 technology?

What is one possible application of the CRISPR-Cas9 system?

What are some ethical and safety considerations associated with the use of CRISPR-Cas9 technology?

Which method is used to deliver mRNA vaccines?

What is the function of zinc finger nucleases (ZFNs) in genome editing?

What is one possible application of the CRISPR-Cas9 system?

Which type of organism was the CRISPR-Cas9 system first observed in?

What does CRISPR stand for?

What is the function of the Cas9 endonuclease in the CRISPR-Cas9 system?

What is one potential application of genome editing techniques?

Which enzyme cleaves double-stranded RNA into approximately 20-25 nucleotide-long siRNA?

What is the role of Argonaute in RNA interference (RNAi)?

What is the function of synthetic siRNAs in gene function analysis?

What is the limitation of RNA interference (RNAi) as a gene silencing technique?

Which of the following is true about the CRISPR-Cas9 system?

Which of the following is a possible application of the CRISPR-Cas9 system?

What is the purpose of the guide RNA in CRISPR-Cas9 technology?

What are some ethical and safety considerations associated with the use of CRISPR-Cas9 technology?

Which repair mechanism is used by cells to fix double-strand breaks made to DNA during genome editing?

What is the role of the protospacer adjacent motif (PAM) in genome editing using CRISPR?

What is one possible application of the CRISPR-Cas9 system?

Which genome editing method uses components of a prokaryotic immune system to target almost any desired genetic sequence?

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