Genetic Testing Methods Quiz
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Questions and Answers

What technology has been developed to screen all chromosomes in a single reaction?

  • Real-time PCR
  • Array Comparative Genome Hybridisation (aCGH) (correct)
  • Transcriptomics
  • RT-PCR
  • Which technique can simultaneously detect aneuploidies, deletions, and duplications?

  • RT-PCR
  • SNP Arrays (correct)
  • NGS (Next-Generation Sequencing)
  • Transcriptomics
  • What method involves mixing denatured DNA from two sources, allowing the identification of specific DNA sequences in one source?

  • Southern blot
  • Fluorescence in situ hybridisation (FISH) (correct)
  • Microarray
  • Dot blot
  • What does a 1:1 ratio of test DNA and control DNA indicate in the Array-CGH test?

    <p>Disomy</p> Signup and view all the answers

    Which method allows simultaneous analysis of expression profiles of thousands of genes?

    <p>Expression Microarray</p> Signup and view all the answers

    In the SNP Arrays, what is indicated by a gain of test DNA relative to controls in a 3:2 ratio?

    <p>Trisomy</p> Signup and view all the answers

    In microarray analysis of gene expression, what does a brighter spot indicate?

    <p>Higher gene expression</p> Signup and view all the answers

    What method involves complete cDNA from a sample being labeled with a fluorescent dye and washed over an array?

    <p>Hybridisation methods</p> Signup and view all the answers

    Which technology can scan a complete genome for imbalances according to the information provided?

    <p>NGS (Next-Generation Sequencing)</p> Signup and view all the answers

    Which technique enables the detection of a cluster of complementary DNA sequences within each dot?

    <p>Dot blot</p> Signup and view all the answers

    What does a gain of test DNA relative to controls in a 3:2 ratio indicate?

    <p>Trisomy</p> Signup and view all the answers

    What allows the binding of complementary DNAs in a sample to their matched probes followed by laser excitation and scanning?

    <p>Microarray</p> Signup and view all the answers

    What is the main feature of Non-Invasive Prenatal Testing (NIPT)?

    <p>It analyzes fetal DNA found in maternal plasma.</p> Signup and view all the answers

    In the context of NIPT, what does a result indicating 'increased risk' suggest about the fetus?

    <p>The fetus might have a rare trisomy that most miscarry before 10-12 weeks.</p> Signup and view all the answers

    What is the primary purpose of RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction)?

    <p>To measure the amount of RNA produced by a gene in a cell.</p> Signup and view all the answers

    What is the main application of NGS (Next-Generation Sequencing) in reproductive biology?

    <p>To analyze maternal plasma DNA for fetal genetic information.</p> Signup and view all the answers

    What does Real-time PCR primarily focus on in molecular analysis?

    <p>Assessing the activity of a gene based on RNA transcripts.</p> Signup and view all the answers

    How does Transcriptomics differ from routine genetic sequencing?

    <p>Transcriptomics involves assessing gene expression through RNA transcripts.</p> Signup and view all the answers

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