Genetic Recombination Technology Quiz
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Questions and Answers

What is the main purpose of inserting recombinant DNA into a host cell?

  • To detect HIV
  • To multiply the DNA in the culture media
  • To express the manufactured protein (correct)
  • To prevent genetic diseases

Which field benefits the most from the application of Recombinant DNA Technology?

  • Industries
  • Agriculture
  • Animal Husbandry
  • Medical Science (correct)

What is the primary use of rDNA technology in agriculture?

  • Detection of genetic diseases
  • Treatment of hereditary diseases
  • Production of GM crops (correct)
  • Protection against worms

Which disease can be detected using Recombinant DNA Technology?

<p>HIV (C)</p> Signup and view all the answers

What is gene therapy in the context of Recombinant DNA Technology?

<p>Treating genetic diseases by correcting gene defects (B)</p> Signup and view all the answers

Which procedure is an example of clinical diagnosis using Recombinant DNA Technology?

<p>ELISA (B)</p> Signup and view all the answers

What is the term for a piece of DNA created by combining at least two strands?

<p>rDNA (B)</p> Signup and view all the answers

What do restriction enzymes do in genetic recombination technology?

<p>Cut DNA at specific locations (C)</p> Signup and view all the answers

What are plasmids in the context of genetic recombination technology?

<p>Vectors carrying the gene of interest to host cells (A)</p> Signup and view all the answers

What is the term for isolating desired DNA, free from other macromolecules?

<p>Steps of Genetic Recombination Technology (B)</p> Signup and view all the answers

Which term describes DNA molecules formed by laboratory methods to bring together genetic material from multiple sources?

<p>Recombinant DNA (rDNA) (D)</p> Signup and view all the answers

What is the role of a 'gene of interest' in genetic recombination technology?

<p>To be inserted into host cells for phenotypic expression (C)</p> Signup and view all the answers

What is the main purpose of Polymerase Chain Reaction (PCR)?

<p>To amplify a single copy of DNA into thousands to millions of copies (C)</p> Signup and view all the answers

What are the components required for a PCR reaction?

<p>DNA template, Primers, DNA polymerase, Nucleotides (D)</p> Signup and view all the answers

What is the function of DNA Ligase in genetic recombination?

<p>To join cut DNA fragments into a plasmid vector (B)</p> Signup and view all the answers

Which enzyme is responsible for extending the primers in PCR?

<p>DNA polymerase (A)</p> Signup and view all the answers

What is the resulting DNA molecule called when a cut fragment of DNA is joined into a plasmid vector?

<p>rDNA or Recombinant DNA molecule (A)</p> Signup and view all the answers

In genetic recombination technology, what is the purpose of using primers?

<p>To identify specific regions on the DNA template (D)</p> Signup and view all the answers

Flashcards

Recombinant DNA (rDNA)

A DNA molecule created in the lab by combining genetic material from different sources.

Restriction enzymes

Enzymes that cut DNA at specific sequences.

Plasmids

Circular DNA molecules that act as vectors, carrying genes to host cells.

Gene of interest

The specific gene that researchers want to insert into a host cell.

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Polymerase Chain Reaction (PCR)

A technique used to amplify a DNA sequence.

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PCR components

DNA template, primers, DNA polymerase, and nucleotides.

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DNA Ligase

An enzyme that joins DNA fragments together.

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DNA polymerase

Enzyme that extends DNA primers.

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rDNA Purpose

Expression of a manufactured protein in a host cell.

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Recombinant DNA Technology application Medical

Most valuable applications are disease treatment.

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Recombinant DNA Technology application Agriculture

Production of genetically modified crops.

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Gene Therapy

Treating genetic diseases by correcting gene defects.

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Clinical Diagnosis (rDNA)

Disease detection using Recombinant DNA Technology.

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ELISA

Example clinical diagnostic using rDNA.

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PCR Primers

Specific DNA fragments that identify target regions in DNA.

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rDNA molecule Result

Combined DNA from different or multiple sources.

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Steps of Genetic Recombination Technology

Isolation of specific DNA sequences, free from other macromolecules.

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