18 Questions
What is the main purpose of inserting recombinant DNA into a host cell?
To express the manufactured protein
Which field benefits the most from the application of Recombinant DNA Technology?
Medical Science
What is the primary use of rDNA technology in agriculture?
Production of GM crops
Which disease can be detected using Recombinant DNA Technology?
HIV
What is gene therapy in the context of Recombinant DNA Technology?
Treating genetic diseases by correcting gene defects
Which procedure is an example of clinical diagnosis using Recombinant DNA Technology?
ELISA
What is the term for a piece of DNA created by combining at least two strands?
rDNA
What do restriction enzymes do in genetic recombination technology?
Cut DNA at specific locations
What are plasmids in the context of genetic recombination technology?
Vectors carrying the gene of interest to host cells
What is the term for isolating desired DNA, free from other macromolecules?
Steps of Genetic Recombination Technology
Which term describes DNA molecules formed by laboratory methods to bring together genetic material from multiple sources?
Recombinant DNA (rDNA)
What is the role of a 'gene of interest' in genetic recombination technology?
To be inserted into host cells for phenotypic expression
What is the main purpose of Polymerase Chain Reaction (PCR)?
To amplify a single copy of DNA into thousands to millions of copies
What are the components required for a PCR reaction?
DNA template, Primers, DNA polymerase, Nucleotides
What is the function of DNA Ligase in genetic recombination?
To join cut DNA fragments into a plasmid vector
Which enzyme is responsible for extending the primers in PCR?
DNA polymerase
What is the resulting DNA molecule called when a cut fragment of DNA is joined into a plasmid vector?
rDNA or Recombinant DNA molecule
In genetic recombination technology, what is the purpose of using primers?
To identify specific regions on the DNA template
Test your knowledge on the steps involved in the insertion of recombinant DNA into a host cell, along with applications of recombinant DNA technology in various fields. Learn about the transformation process and expression of manufactured proteins through rDNA.
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