Genetic Recombination in Microbiology
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A ________________ cell can take up and incorporate foreign DNA from the surrounding environment in a process called transformation

prokaryotic

________________________________ is the movement of genes between bacteria by bacteriophages (viruses that infect bacteria)

Transduction

Movement of genes among individuals from different species is called ____________________ gene transfer

horizontal

______________________ is the process where genetic material is transferred between prokaryotic cells

<p>Conjugation</p> Signup and view all the answers

Phototrophs obtain energy from ____________________

<p>light</p> Signup and view all the answers

Nitrogen is essential for the production of ____________________ acids and nucleic acids

<p>amino</p> Signup and view all the answers

In nitrogen fixation, some prokaryotes convert atmospheric ____________________ (N2) to ammonia (NH3)

<p>nitrogen</p> Signup and view all the answers

Obligate ____________________ are poisoned by O2 and use fermentation or anaerobic respiration

<p>anaerobes</p> Signup and view all the answers

Some archaea live in ______________ environments and are called extremophiles

<p>extreme</p> Signup and view all the answers

Methanogens are ______________ anaerobes and are poisoned by O2

<p>strict</p> Signup and view all the answers

Chlamydia are ______________ that live only within animal cells

<p>parasites</p> Signup and view all the answers

Treponema pallidum causes ______________ by sexual transmission

<p>syphilis</p> Signup and view all the answers

Cyanobacteria are ______________ that generate O2

<p>photoautotrophs</p> Signup and view all the answers

Bacillus anthracis is the cause of ______________

<p>anthrax</p> Signup and view all the answers

Prokaryotes can increase the availability of ______________, phosphorus, and potassium for plant growth

<p>nitrogen</p> Signup and view all the answers

Symbiosis is an ecological relationship in which two species live in close contact: a larger ______________ and smaller symbiont

<p>host</p> Signup and view all the answers

Some prokaryotes have ______ which allow them to stick to their substrate or other individuals in a colony

<p>fimbriae</p> Signup and view all the answers

Pili (or sex ______) are longer than fimbriae and allow prokaryotes to exchange DNA

<p>pili</p> Signup and view all the answers

Many bacteria exhibit ______, the ability to move toward or away from a stimulus

<p>taxis</p> Signup and view all the answers

Chemotaxis is the movement toward or away from a ______ stimulus

<p>chemical</p> Signup and view all the answers

Most motile bacteria propel themselves by ______ scattered about the surface or concentrated at one or both ends

<p>flagella</p> Signup and view all the answers

Bacterial ______ are composed of a motor, hook, and filament

<p>flagella</p> Signup and view all the answers

Many prokaryotes form metabolically inactive ______, which can remain viable in harsh conditions for centuries

<p>endospores</p> Signup and view all the answers

Three factors contribute to their genetic diversity: Rapid reproduction, ______, and Genetic recombination

<p>mutation</p> Signup and view all the answers

Study Notes

Genetic Recombination

  • Prokaryotic cells can take up and incorporate foreign DNA from the surrounding environment through transformation.
  • Transduction is the movement of genes between bacteria by bacteriophages (viruses that infect bacteria).
  • Horizontal gene transfer refers to the movement of genes among individuals from different species.
  • Conjugation is the process where genetic material is transferred between prokaryotic cells.

Conjugation

  • In bacteria, DNA transfer is one-way, from a donor cell to a recipient cell.
  • A donor cell attaches to a recipient by a pilus, pulls it closer, and transfers DNA.
  • The F factor is required for the production of pili.

Metabolism and Nutrition

  • Prokaryotes can be categorized by how they obtain energy and carbon:
    • Phototrophs obtain energy from light.
    • Chemotrophs obtain energy from chemicals.
    • Autotrophs require CO2 as a carbon source.
    • Heterotrophs require an organic nutrient to make organic compounds.

Nutrition Modes

  • Energy and carbon sources are combined to give four major modes of nutrition.

Nitrogen Metabolism

  • Nitrogen is essential for the production of amino acids and nucleic acids.
  • In nitrogen fixation, some prokaryotes convert atmospheric nitrogen (N2) to ammonia (NH3).
  • In the cyanobacterium Anabaena, photosynthetic cells and nitrogen-fixing cells called heterocysts exchange metabolic products.

Prokaryotic Metabolism

  • Obligate aerobes require O2 for cellular respiration.
  • Obligate anaerobes are poisoned by O2 and use fermentation or anaerobic respiration.
  • Facultative anaerobes can survive with or without O2.

Prokaryotic Diversity

Domain Archaea

  • Archaea share certain traits with bacteria and other traits with eukaryotes.
  • Some archaea live in extreme environments and are called extremophiles.
  • Extreme halophiles live in highly saline environments.
  • Extreme thermophiles thrive in very hot environments.
  • Methanogens produce methane as a waste product.

Domain Bacteria

  • Proteobacteria include photoautotrophs, chemoautotrophs, and heterotrophs.
  • Some proteobacteria are anaerobic, and others are aerobic.
  • Chlamydia are parasites that live only within animal cells.
  • Spirochetes are helical heterotrophs.
  • Cyanobacteria are photoautotrophs that generate O2.
  • Gram-positive bacteria include actinomycetes, Bacillus anthracis, Clostridium botulinum, and some Staphylococcus and Streptococcus.

Ecological Importance

  • Prokaryotes play a major role in the recycling of chemical elements between the living and nonliving components of ecosystems.
  • Chemoheterotrophic prokaryotes function as decomposers, breaking down dead organisms and waste products.
  • Prokaryotes can increase the availability of nitrogen, phosphorus, and potassium for plant growth.

Symbiosis

  • Symbiosis is an ecological relationship in which two species live in close contact: a larger host and smaller symbiont.
  • Some prokaryotes have fimbriae, which allow them to stick to their substrate or other individuals in a colony.
  • Pili (or sex pili) are longer than fimbriae and allow prokaryotes to exchange DNA.

Locomotion

  • Many bacteria exhibit taxis, the ability to move toward or away from a stimulus.
  • Chemotaxis is the movement toward or away from a chemical stimulus.
  • Most motile bacteria propel themselves by flagella scattered about the surface or concentrated at one or both ends.
  • Bacterial flagella are composed of a motor, hook, and filament.

Internal Organization

  • Prokaryotic cells usually lack complex compartmentalization.
  • Some prokaryotes have in-foldings of the plasma membrane that perform metabolic functions.
  • Most of the genome consists of a circular chromosome located in the nucleoid region.
  • Some species of bacteria also have smaller rings of DNA called plasmids.

Prokaryotic Reproduction

  • Bacteria are small and reproduce by binary fission (1-3 hours).
  • They have short generation times.
  • Many prokaryotes form metabolically inactive endospores, which can remain viable in harsh conditions for centuries.
  • Three factors contribute to genetic diversity in bacteria: rapid reproduction, mutation, and genetic recombination.
  • Rapid reproduction and mutation allow for rapid evolution.
  • Genetic recombination through conjugation, transduction, and transformation increases genetic diversity in bacteria.

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Bacteria and Archaea.pptx

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This quiz covers the different methods of genetic recombination in bacteria, including transformation, transduction, horizontal gene transfer, and conjugation.

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