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Questions and Answers
What is the purpose of introducing a mismatch in the primer?
What is the purpose of introducing a mismatch in the primer?
- To eliminate the HindIII recognition site
- To introduce a HindIII recognition site (correct)
- To prevent PCR amplification
- To increase the PCR product yield
What does the black sequence in the figure represent?
What does the black sequence in the figure represent?
- The PCR product
- Two corn plants of the same strain with different SNPs (correct)
- The original corn plant genotype
- The HindIII recognition site
What is the color and significance of the forward primer in the figure?
What is the color and significance of the forward primer in the figure?
- Colored blue and introduces a different DNA sequence at the mismatch (correct)
- Colored black and introduces a HindIII recognition site
- Colored red and eliminates the HindIII recognition site
- Colored green and prevents PCR amplification
