Genetic Engineering Methods Quiz

Bacterial Artificial Chromosome (BAC)

phage vectors

Yeast Artificial Chromosome (YAC)

<p>cloning vector</p> Signup and view all the answers

<p>Phage vectors, like Lambda phage vectors, are modified versions of bacteriophages designed to undergo the lysogenic cycle, allowing for the insertion of target genes into the bacterial chromosome. BACs, on the other hand, are derived from bacterial chromosomes and have a large carrying capacity for very large DNA segments, used for cloning large DNA sequences.</p> Signup and view all the answers

<p>Expression vectors</p> Signup and view all the answers

<p>YACs are derived from yeast cells and can carry large eukaryotic DNA segments. They have the advantage of being able to express these segments in eukaryotic cells.</p> Signup and view all the answers

<p>Expression vectors are used to express cloned DNA sequences. They often contain regulatory sequences to control gene expression and are designed to produce specific proteins or RNA molecules.</p> Signup and view all the answers

<p>Phage vectors, like Lambda phage vectors, are designed to undergo the lysogenic cycle, where the viral DNA is integrated into the host cell's chromosome and replicated along with the host DNA. This is different from the lytic cycle, where the virus replicates and destroys the host cell to release new viral particles.</p> Signup and view all the answers

<p>The choice of vector depends on specific cloning needs, such as the size of the DNA segment and the method of delivery. Each cloning vector has its own advantages and disadvantages.</p> Signup and view all the answers