10 Questions
______: BACs are derived from bacterial chromosomes and have a large carrying capacity for very large DNA segments. They are used for cloning large DNA sequences
Bacterial Artificial Chromosome (BAC)
Phage vectors, like Lambda ______, are modified versions of bacteriophages. They are designed to only undergo the lysogenic cycle and not the lytic cycle, allowing for the insertion of target genes into the bacterial chromosome
phage vectors
______: YACs are derived from yeast cells and can also carry large eukaryotic DNA segments. They have the advantage of being able to express these segments in eukaryotic cells
Yeast Artificial Chromosome (YAC)
Each ______ has its own advantages and disadvantages, and the choice of vector depends on the specific cloning needs, such as the size of the DNA segment and the method of delivery
cloning vector
Explain the difference between phage vectors and bacterial artificial chromosomes (BACs) in DNA cloning techniques.
Phage vectors, like Lambda phage vectors, are modified versions of bacteriophages designed to undergo the lysogenic cycle, allowing for the insertion of target genes into the bacterial chromosome. BACs, on the other hand, are derived from bacterial chromosomes and have a large carrying capacity for very large DNA segments, used for cloning large DNA sequences.
______, such as the PET vec
Expression vectors
What are the advantages of using yeast artificial chromosomes (YACs) in DNA cloning?
YACs are derived from yeast cells and can carry large eukaryotic DNA segments. They have the advantage of being able to express these segments in eukaryotic cells.
Explain the role of expression vectors in genetic engineering.
Expression vectors are used to express cloned DNA sequences. They often contain regulatory sequences to control gene expression and are designed to produce specific proteins or RNA molecules.
How does the lysogenic cycle of phage vectors differ from the lytic cycle?
Phage vectors, like Lambda phage vectors, are designed to undergo the lysogenic cycle, where the viral DNA is integrated into the host cell's chromosome and replicated along with the host DNA. This is different from the lytic cycle, where the virus replicates and destroys the host cell to release new viral particles.
What factors influence the choice of cloning vector in genetic engineering?
The choice of vector depends on specific cloning needs, such as the size of the DNA segment and the method of delivery. Each cloning vector has its own advantages and disadvantages.
Test your knowledge of genetic engineering methods by taking this quiz. Explore topics such as bacterial transformation, cosmid-mediated processes, phage vectors, and bacterial artificial chromosomes (BACs).
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