Genetic Engineering in General Biology II
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Questions and Answers

What is the primary purpose of using enzymes to separate and purify DNA from other macromolecules within a cell?

  • To remove impurities and isolate the pure DNA for further analysis (correct)
  • To selectively amplify a specific DNA sequence
  • To increase the concentration of DNA in the sample
  • To break down the cell membrane and release the DNA

    • What is the role of DNA ligase in the process of recombinant DNA technology?

  • To join the cut DNA fragment and the vector of interest together (correct)
  • To cut the DNA at specific locations using restriction enzymes
  • To amplify a single DNA sequence into multiple copies using PCR
  • To make the recipient host cell 'capable' of accepting the recombinant DNA

    • What is the purpose of the 'Agarose Gel Electrophoresis' technology in the context of recombinant DNA technology?

  • To amplify a single DNA sequence into multiple copies using PCR
  • To join the cut DNA fragment and the vector of interest together
  • To separate and purify the DNA from other macromolecules
  • To separate and identify the different sizes of DNA fragments after digestion with restriction enzymes (correct)

    • Why do bacterial cells require special treatments to accept foreign DNA during the transformation process in recombinant DNA technology?

    <p>All of the above (D)</p> Signup and view all the answers

    What is the primary purpose of the Polymerase Chain Reaction (PCR) technique in the context of recombinant DNA technology?

    <p>To amplify a single DNA sequence into thousands or millions of copies (B)</p> Signup and view all the answers

    What is the primary purpose of genetic engineering?

    <p>To create new organisms by combining DNA from different sources (C)</p> Signup and view all the answers

    Which of the following is NOT a step in the genetic engineering process?

    <p>Sequencing the entire genome of the host organism (D)</p> Signup and view all the answers

    What is the role of a vector genome in genetic engineering?

    <p>It serves as a vehicle to carry the isolated DNA into the host cell (A)</p> Signup and view all the answers

    Why are bacteria commonly used as host organisms in genetic engineering?

    <p>They can be grown in large numbers relatively quickly and easily (A)</p> Signup and view all the answers

    What is the purpose of isolating DNA in the genetic engineering process?

    <p>All of the above (D)</p> Signup and view all the answers

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