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Genetic Engineering in General Biology II

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Genetic Engineering in General Biology II

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Questions and Answers

What is the primary purpose of using enzymes to separate and purify DNA from other macromolecules within a cell?

To remove impurities and isolate the pure DNA for further analysis (correct)

To selectively amplify a specific DNA sequence

To increase the concentration of DNA in the sample

To break down the cell membrane and release the DNA

What is the role of DNA ligase in the process of recombinant DNA technology?

To join the cut DNA fragment and the vector of interest together (correct)

To cut the DNA at specific locations using restriction enzymes

To amplify a single DNA sequence into multiple copies using PCR

To make the recipient host cell 'capable' of accepting the recombinant DNA

What is the purpose of the 'Agarose Gel Electrophoresis' technology in the context of recombinant DNA technology?

To amplify a single DNA sequence into multiple copies using PCR

To join the cut DNA fragment and the vector of interest together

To separate and purify the DNA from other macromolecules

To separate and identify the different sizes of DNA fragments after digestion with restriction enzymes (correct)

Why do bacterial cells require special treatments to accept foreign DNA during the transformation process in recombinant DNA technology?

All of the above Signup and view all the answers

What is the primary purpose of the Polymerase Chain Reaction (PCR) technique in the context of recombinant DNA technology?

To amplify a single DNA sequence into thousands or millions of copies Signup and view all the answers

What is the primary purpose of genetic engineering?

To create new organisms by combining DNA from different sources Signup and view all the answers

Which of the following is NOT a step in the genetic engineering process?

Sequencing the entire genome of the host organism Signup and view all the answers

What is the role of a vector genome in genetic engineering?

It serves as a vehicle to carry the isolated DNA into the host cell Signup and view all the answers

Why are bacteria commonly used as host organisms in genetic engineering?

They can be grown in large numbers relatively quickly and easily Signup and view all the answers

What is the purpose of isolating DNA in the genetic engineering process?