Genetic Engineering and Restriction Enzymes
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Questions and Answers

What is the primary purpose of restriction mapping?

  • To produce recombinant DNA molecules
  • To determine the length of a DNA fragment
  • To analyze the sequence of a DNA fragment
  • To identify the relative locations of restriction enzyme cutting sites (correct)
  • What is the function of DNA ligase in DNA modification?

  • To join DNA fragments (correct)
  • To replicate DNA
  • To cut DNA fragments
  • To translate DNA into RNA
  • How many fragments are produced by each single enzyme reaction in the example shown in Table 4.2?

  • Three
  • Four
  • One
  • Two (correct)
  • What is the length of the DNA fragment used in the example shown in Table 4.2?

    <p>15 kbp</p> Signup and view all the answers

    What is the purpose of running the fragments on an agarose gel?

    <p>To determine the size of the fragments</p> Signup and view all the answers

    What is the name of the technique used to obtain information about the relative locations of restriction enzyme cutting sites?

    <p>Restriction mapping</p> Signup and view all the answers

    What type of enzymes are used to cut DNA fragments in restriction mapping?

    <p>Restriction enzymes</p> Signup and view all the answers

    What is the outcome of the triple digest in the example shown in Table 4.2?

    <p>It confirms the restriction map</p> Signup and view all the answers

    What is the function of restriction endonucleases in genetic engineering?

    <p>To cut DNA at specific sequences</p> Signup and view all the answers

    What fragments are produced from a 15 kb fragment treated with BamHI?

    <p>14 kb and 1 kb</p> Signup and view all the answers

    Which of the following nucleases is an endonuclease?

    <p>Deoxyribonuclease I (DNase I)</p> Signup and view all the answers

    What are ribonucleases (RNases) primarily used for?

    <p>To degrade RNA</p> Signup and view all the answers

    How do exonucleases differ from endonucleases?

    <p>Exonucleases degrade DNA from the termini, while endonucleases cut within the DNA strand</p> Signup and view all the answers

    What are the fragment sizes produced from a BamHI/EcoRI double digest of the 15 kb fragment?

    <p>11 kb, 3 kb, and 1 kb</p> Signup and view all the answers

    Which of the following is NOT a type of nuclease enzyme mentioned in the content?

    <p>Polymerase I</p> Signup and view all the answers

    What is the ultimate use of ribonucleases in genetic engineering?

    <p>To get rid of unwanted RNA in the preparation</p> Signup and view all the answers

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