Podcast
Questions and Answers
What is the primary purpose of restriction mapping?
What is the primary purpose of restriction mapping?
- To produce recombinant DNA molecules
- To determine the length of a DNA fragment
- To analyze the sequence of a DNA fragment
- To identify the relative locations of restriction enzyme cutting sites (correct)
What is the function of DNA ligase in DNA modification?
What is the function of DNA ligase in DNA modification?
- To join DNA fragments (correct)
- To replicate DNA
- To cut DNA fragments
- To translate DNA into RNA
How many fragments are produced by each single enzyme reaction in the example shown in Table 4.2?
How many fragments are produced by each single enzyme reaction in the example shown in Table 4.2?
- Three
- Four
- One
- Two (correct)
What is the length of the DNA fragment used in the example shown in Table 4.2?
What is the length of the DNA fragment used in the example shown in Table 4.2?
What is the purpose of running the fragments on an agarose gel?
What is the purpose of running the fragments on an agarose gel?
What is the name of the technique used to obtain information about the relative locations of restriction enzyme cutting sites?
What is the name of the technique used to obtain information about the relative locations of restriction enzyme cutting sites?
What type of enzymes are used to cut DNA fragments in restriction mapping?
What type of enzymes are used to cut DNA fragments in restriction mapping?
What is the outcome of the triple digest in the example shown in Table 4.2?
What is the outcome of the triple digest in the example shown in Table 4.2?
What is the function of restriction endonucleases in genetic engineering?
What is the function of restriction endonucleases in genetic engineering?
What fragments are produced from a 15 kb fragment treated with BamHI?
What fragments are produced from a 15 kb fragment treated with BamHI?
Which of the following nucleases is an endonuclease?
Which of the following nucleases is an endonuclease?
What are ribonucleases (RNases) primarily used for?
What are ribonucleases (RNases) primarily used for?
How do exonucleases differ from endonucleases?
How do exonucleases differ from endonucleases?
What are the fragment sizes produced from a BamHI/EcoRI double digest of the 15 kb fragment?
What are the fragment sizes produced from a BamHI/EcoRI double digest of the 15 kb fragment?
Which of the following is NOT a type of nuclease enzyme mentioned in the content?
Which of the following is NOT a type of nuclease enzyme mentioned in the content?
What is the ultimate use of ribonucleases in genetic engineering?
What is the ultimate use of ribonucleases in genetic engineering?
