Questions and Answers
What is the primary purpose of restriction mapping?
To identify the relative locations of restriction enzyme cutting sites
What is the function of DNA ligase in DNA modification?
To join DNA fragments
How many fragments are produced by each single enzyme reaction in the example shown in Table 4.2?
Two
What is the length of the DNA fragment used in the example shown in Table 4.2?
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What is the purpose of running the fragments on an agarose gel?
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What is the name of the technique used to obtain information about the relative locations of restriction enzyme cutting sites?
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What type of enzymes are used to cut DNA fragments in restriction mapping?
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What is the outcome of the triple digest in the example shown in Table 4.2?
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What is the function of restriction endonucleases in genetic engineering?
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What fragments are produced from a 15 kb fragment treated with BamHI?
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Which of the following nucleases is an endonuclease?
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What are ribonucleases (RNases) primarily used for?
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How do exonucleases differ from endonucleases?
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What are the fragment sizes produced from a BamHI/EcoRI double digest of the 15 kb fragment?
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Which of the following is NOT a type of nuclease enzyme mentioned in the content?
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What is the ultimate use of ribonucleases in genetic engineering?
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